{"title":"超声波和微波辅助水蒸馏法强化丁香精油萃取及其抗氧化活性比较","authors":"Panawan Suttiarporn , Thidaporn Seangwattana , Thanarak Srisurat , Kanokchaya Kongitthinon , Napasawan Chumnanvej , Suwaporn Luangkamin","doi":"10.1016/j.crgsc.2024.100411","DOIUrl":null,"url":null,"abstract":"<div><p>Clove essential oil (CEO) is recognized for its variety of bioactivities and is widely used in the fields of food and pharmaceuticals. Eugenol is the main component that causes the oil's effectiveness. This study included a bibliometric analysis to demonstrate the novelty of the CEO extraction method. A combination of ultrasound and microwave techniques was utilized to enhance the efficiency of hydrodistillation (HD) extraction of essential oil from clove bud in terms of extraction yield, extraction rate, and eugenol composition, which is a strong antioxidant agent. Meanwhile, the effects of ultrasonic extraction temperature and time, microwave distillation power and time, and the ratio of clove to water were also investigated. The optimum conditions were as follows: ultrasonic extraction was at 40 °C for 45 min, microwave distillation power was 400 W for 45 min, and the clove to water ratio was 1:20 (g/mL). The ultrasound pretreatment prior to microwave-assisted hydrodistillation (U-MHD) method obtained the CEO, which had a significantly higher extraction yield (16.80 ± 0.26 %) and a greater amount of eugenol (78.49 %) than the CEOs obtained by traditional HD and MHD methods. Corresponding to the antioxidant activity of CEO from U-MHD, it possessed the strongest DPPH radical scavenging activity with an IC<sub>50</sub> value of 2.79 ± 0.05 μg/mL, and it was comparable to the activity of eugenol. This is the first occurrence of CEO extraction employing the U-MHD procedure. This process could be used to enhance the bioactivity of CEO as well as its extraction efficiency, easy operation, rapid extraction, and eco-friendly technique. In terms of cost-analysis, MHD stands out as the most economically viable extraction method, boasting the lowest cost per 100 g product. The obtained results serve as fundamental data for implementing the scaling up of extraction processes to an industrial level.</p></div>","PeriodicalId":296,"journal":{"name":"Current Research in Green and Sustainable Chemistry","volume":"8 ","pages":"Article 100411"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266608652400016X/pdfft?md5=400ef9b0fd9288b339da3e66b6146f60&pid=1-s2.0-S266608652400016X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Enhanced extraction of clove essential oil by ultrasound and microwave assisted hydrodistillation and their comparison in antioxidant activity\",\"authors\":\"Panawan Suttiarporn , Thidaporn Seangwattana , Thanarak Srisurat , Kanokchaya Kongitthinon , Napasawan Chumnanvej , Suwaporn Luangkamin\",\"doi\":\"10.1016/j.crgsc.2024.100411\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Clove essential oil (CEO) is recognized for its variety of bioactivities and is widely used in the fields of food and pharmaceuticals. Eugenol is the main component that causes the oil's effectiveness. This study included a bibliometric analysis to demonstrate the novelty of the CEO extraction method. A combination of ultrasound and microwave techniques was utilized to enhance the efficiency of hydrodistillation (HD) extraction of essential oil from clove bud in terms of extraction yield, extraction rate, and eugenol composition, which is a strong antioxidant agent. Meanwhile, the effects of ultrasonic extraction temperature and time, microwave distillation power and time, and the ratio of clove to water were also investigated. The optimum conditions were as follows: ultrasonic extraction was at 40 °C for 45 min, microwave distillation power was 400 W for 45 min, and the clove to water ratio was 1:20 (g/mL). The ultrasound pretreatment prior to microwave-assisted hydrodistillation (U-MHD) method obtained the CEO, which had a significantly higher extraction yield (16.80 ± 0.26 %) and a greater amount of eugenol (78.49 %) than the CEOs obtained by traditional HD and MHD methods. Corresponding to the antioxidant activity of CEO from U-MHD, it possessed the strongest DPPH radical scavenging activity with an IC<sub>50</sub> value of 2.79 ± 0.05 μg/mL, and it was comparable to the activity of eugenol. This is the first occurrence of CEO extraction employing the U-MHD procedure. This process could be used to enhance the bioactivity of CEO as well as its extraction efficiency, easy operation, rapid extraction, and eco-friendly technique. In terms of cost-analysis, MHD stands out as the most economically viable extraction method, boasting the lowest cost per 100 g product. The obtained results serve as fundamental data for implementing the scaling up of extraction processes to an industrial level.</p></div>\",\"PeriodicalId\":296,\"journal\":{\"name\":\"Current Research in Green and Sustainable Chemistry\",\"volume\":\"8 \",\"pages\":\"Article 100411\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S266608652400016X/pdfft?md5=400ef9b0fd9288b339da3e66b6146f60&pid=1-s2.0-S266608652400016X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Research in Green and Sustainable Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S266608652400016X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Materials Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Research in Green and Sustainable Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S266608652400016X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Materials Science","Score":null,"Total":0}
引用次数: 0
摘要
丁香精油（CEO）具有多种生物活性，被广泛应用于食品和医药领域。丁香酚是导致精油功效的主要成分。这项研究包括一项文献计量分析，以证明 CEO 提取方法的新颖性。在萃取率、萃取率和丁香酚成分（丁香酚是一种强抗氧化剂）方面，研究人员利用超声波和微波技术相结合的方法提高了水蒸馏（HD）萃取丁香芽精油的效率。同时，还研究了超声波萃取温度和时间、微波蒸馏功率和时间以及丁香与水的比例的影响。最佳条件如下：超声波提取温度为 40 °C，时间为 45 分钟；微波蒸馏功率为 400 W，时间为 45 分钟；丁香与水的比例为 1:20（克/毫升）。在微波辅助水蒸馏法（U-MHD）之前进行超声波预处理可获得 CEO，其提取率（16.80 ± 0.26 %）和丁香酚含量（78.49 %）均显著高于传统 HD 和 MHD 法获得的 CEO。与 U-MHD 法提取的 CEO 的抗氧化活性相对应，它具有最强的 DPPH 自由基清除活性，IC50 值为 2.79 ± 0.05 μg/mL，与丁香酚的活性相当。这是首次采用 U-MHD 程序进行 CEO 提取。该工艺具有提取效率高、操作简便、提取速度快、环保等优点，可用于提高 CEO 的生物活性。从成本分析来看，MHD 是最经济可行的提取方法，每 100 克产品的成本最低。所获得的结果是将萃取工艺提升到工业水平的基础数据。
Enhanced extraction of clove essential oil by ultrasound and microwave assisted hydrodistillation and their comparison in antioxidant activity
Clove essential oil (CEO) is recognized for its variety of bioactivities and is widely used in the fields of food and pharmaceuticals. Eugenol is the main component that causes the oil's effectiveness. This study included a bibliometric analysis to demonstrate the novelty of the CEO extraction method. A combination of ultrasound and microwave techniques was utilized to enhance the efficiency of hydrodistillation (HD) extraction of essential oil from clove bud in terms of extraction yield, extraction rate, and eugenol composition, which is a strong antioxidant agent. Meanwhile, the effects of ultrasonic extraction temperature and time, microwave distillation power and time, and the ratio of clove to water were also investigated. The optimum conditions were as follows: ultrasonic extraction was at 40 °C for 45 min, microwave distillation power was 400 W for 45 min, and the clove to water ratio was 1:20 (g/mL). The ultrasound pretreatment prior to microwave-assisted hydrodistillation (U-MHD) method obtained the CEO, which had a significantly higher extraction yield (16.80 ± 0.26 %) and a greater amount of eugenol (78.49 %) than the CEOs obtained by traditional HD and MHD methods. Corresponding to the antioxidant activity of CEO from U-MHD, it possessed the strongest DPPH radical scavenging activity with an IC50 value of 2.79 ± 0.05 μg/mL, and it was comparable to the activity of eugenol. This is the first occurrence of CEO extraction employing the U-MHD procedure. This process could be used to enhance the bioactivity of CEO as well as its extraction efficiency, easy operation, rapid extraction, and eco-friendly technique. In terms of cost-analysis, MHD stands out as the most economically viable extraction method, boasting the lowest cost per 100 g product. The obtained results serve as fundamental data for implementing the scaling up of extraction processes to an industrial level.
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