佛罗里达州入侵的巴西胡椒树(Schinus terebinthifolia)中胆诱导真菌的分离和再接种

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Dale A. Halbritter, Min B. Rayamajhi, Paul Madeira, Jorge G. Leidi, Telmah Telmadarrehei, Carey Minteer
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引用次数: 0

摘要

在佛罗里达州南部的田间地头,高度入侵的巴西胡椒树(Schinus terebinthifolia)上出现了大量的茎瘿和类似金缕梅的生长,当地正在释放一种蓟马生物控制剂(Pseudophilothrips ichini),以降低该植物的入侵潜力。在苗圃中种植的盆栽植物上也观察到了瘿。在此,我们的目标是分离和鉴定虫瘿的病原体,并评估其诱导幼稚植物产生虫瘿的能力。我们从田间和苗圃种植的植物中获得了茎瘿,在酸性马铃薯葡萄糖琼脂中无菌分离出真菌,并纯化了真菌菌落。将纯化菌落中的纯化菌丝片段伤口接种到盆栽天真树苗的茎上,很容易诱发与田间和苗圃中观察到的相同的虫瘿。我们证明这种 Cophinforma sp. 可以通过机械伤口感染 S. terebinthifolia 的茎,并在 3 个月内诱发树苗出现明显的茎瘿。这将作为产生虫瘿植物的模型,用于评估诱导虫瘿的真菌对 S. terebinthifolia 的影响,并有可能进一步研究蓟马与这种归化真菌之间的相互作用,这可能会协同和/或增加 S. terebinthifolia 的管理效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and reinoculation of a gall-inducing fungus in the invasive Brazilian peppertree (Schinus terebinthifolia) in Florida
Stem galls and witch’s broom–like growths are locally abundant on the highly invasive Brazilian peppertree (Schinus terebinthifolia) at field sites in southern Florida where a thrips biological control agent (Pseudophilothrips ichini) is being released to reduce the invasive potential of the plant. Galls have also been observed on potted plants in nursery stock grown to feed laboratory colonies of the agent. Herein, our objective was to isolate and identify the causal agent of the galls and assess its ability to induce galls in naive plants. We obtained stem galls from both field- and nursery-grown plants, aseptically isolated a fungus in acidic potato dextrose agar, and purified fungal colonies. Stems of potted naive saplings were wound-inoculated with purified hyphal fragments from the purified colonies, which readily induced galls like those observed in the field and nursery. Simultaneous molecular analysis of the fungal DNA obtained from the galls of field and nursery plants, experimentally induced galls, and fungal colony isolates identified this gall-inducing fungus as Cophinforma sp. We demonstrated that this Cophinforma sp. can infect S. terebinthifolia stems via mechanical wounds and induce visibly discernible stem galls in saplings within 3 mo. This will serve as a model for galled plant production for assessing the impacts of the gall-inducing fungus on S. terebinthifolia, with potential for further study to investigate interactions between the thrips and this naturalized fungus, which may synergistically and/or additively enhance S. terebinthifolia management efficacy.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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