利用细菌敏感微粒和可分离泡腾微阵列贴片组合选择性地向糖尿病足感染细菌投放克林霉素

IF 3.5 3区 医学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Nurul Fauziah, Nur Annisa Safirah, Iis Nurul Rahmadani, Muhammad Nur Hidayat, Nur Azizah Fadhilah, Nana Juniarti Natsir Djide, Andi Dian Permana
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引用次数: 0

摘要

导言糖尿病足感染(DFI)是糖尿病的并发症之一。本研究利用聚己内酯(PCL)聚合物将克林霉素封装在细菌敏感微粒系统(MP-CLY)中。结果 MP-CLY 的表征结果表明,随着 PCL 聚合物用量的增加,CLY 被大量封装,而且 CLY 的化学结构没有发生变化。体外释放测试结果表明,在培养金黄色葡萄球菌的培养基中,CLY 的释放量增加,并呈现出控制释放的效果。MPCLY-SEMAP 的表征结果表明,所开发的配方具有最佳的机械和渗透能力,可在 56 ± 5.099 秒内分离。在受细菌感染的皮肤模型上进行的体外皮肤动力学测试表明,MP-CLY SEMAP 改善了 CLY 的皮肤动力学特征,细菌存活率降低了 99.99%。这项研究的结果可用于今后的研究,在适当的动物模型中对 MP-CLY-SEMAP 进行体内测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Selective Delivery of Clindamycin Using a Combination of Bacterially Sensitive Microparticle and Separable Effervescent Microarray Patch on Bacteria Causing Diabetic Foot Infection

Selective Delivery of Clindamycin Using a Combination of Bacterially Sensitive Microparticle and Separable Effervescent Microarray Patch on Bacteria Causing Diabetic Foot Infection

Introduction

Diabetic foot infection (DFI) is one of the complications of diabetes mellitus. Clindamycin (CLY) is one of the antibiotics recommended to treat DFI, but CLY given orally and intravenously still causes many side effects.

Methods

In this study, we encapsulated CLY in a bacteria sensitive microparticle system (MP-CLY) using polycaprolactone (PCL) polymer. MP-CLY was then delivered in a separable effervescent microarray patch (MP-CLY-SEMAP), which has the ability to separate between the needle layer and separable layer due to the formation of air bubbles when interacting with interstitial fluid in the skin.

Result

The characterization results of MP-CLY proved that CLY was encapsulated in large amounts as the amount of PCL polymer used increased, and there was no change in the chemical structure of CLY. In vitro release test results showed increased CLY release in media cultured with Staphylococcus aureus bacteria and showed controlled release. The characterization results of MPCLY-SEMAP showed that the developed formula has optimal mechanical and penetration capabilities and can separate in 56 ± 5.099 s. An ex vivo dermatokinetic test on a bacterially infected skin model showed an improvement of CLY dermatokinetic profile from MP-CLY SEMAP and a decrease in bacterial viability by 99.99%.

Conclusion

This research offers proof of concept demonstrating the improved dermatokinetic profile of CLY encapsulated in a bacteria sensitive MP form and delivered via MP-CLY-SEMAP. The results of this research can be developed for future research by testing MP-CLY-SEMAP in vivo in appropriate animal models.

Graphical Abstract

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来源期刊
Pharmaceutical Research
Pharmaceutical Research 医学-化学综合
CiteScore
6.60
自引率
5.40%
发文量
276
审稿时长
3.4 months
期刊介绍: Pharmaceutical Research, an official journal of the American Association of Pharmaceutical Scientists, is committed to publishing novel research that is mechanism-based, hypothesis-driven and addresses significant issues in drug discovery, development and regulation. Current areas of interest include, but are not limited to: -(pre)formulation engineering and processing- computational biopharmaceutics- drug delivery and targeting- molecular biopharmaceutics and drug disposition (including cellular and molecular pharmacology)- pharmacokinetics, pharmacodynamics and pharmacogenetics. Research may involve nonclinical and clinical studies, and utilize both in vitro and in vivo approaches. Studies on small drug molecules, pharmaceutical solid materials (including biomaterials, polymers and nanoparticles) biotechnology products (including genes, peptides, proteins and vaccines), and genetically engineered cells are welcome.
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