microRNA-2184通过调节syt3协调斑马鱼毛特纳细胞轴突再生

IF 6.6 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Genetics and Genomics Pub Date : 2024-04-04 DOI:10.1016/j.jgg.2024.03.016

Xinghan Chen, Yueru Shen, Zheng Song, Xinliang Wang, Huaitong Yao, Yuan Cai, Ziang Zhao, Bing Hu

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引用次数: 0

摘要

微RNA（miRNA）在脊髓损伤后的轴突再生中发挥着重要作用。然而，许多 miRNA 在中枢神经系统（CNS）轴突再生中的功能在很大程度上仍未得到探索。在这里，我们阐明了 miR-2184 在斑马鱼毛氏细胞（M 细胞）轴突再生中的积极作用。miR-2184在单个M细胞中的上调促进了轴突再生，而特定海绵诱导的miR-2184沉默则导致轴突再生受阻。我们的研究表明，miR-2184 的下游靶标 miR-2184 负向调控轴突再生，而抑制轴突再生取决于 miR-2184 与 Ca 的结合。此外，药物刺激 cAMP/PKA 通路表明，易释放池的变化可能会影响轴突再生。我们的数据表明，miR-2184 通过调节下游靶点促进了中枢神经系统内 M 细胞的轴突再生，为潜在的治疗策略提供了有价值的见解。

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microRNA-2184 orchestrates Mauthner-cell axon regeneration in zebrafish via syt3 modulation

MicroRNAs (miRNAs) play a significant role in axon regeneration following spinal cord injury. However, the functions of numerous miRNAs in axon regeneration within the central nervous system (CNS) remain largely unexplored. Here, we elucidate the positive role of miR-2184 in axon regeneration within zebrafish Mauthner cells (M-cells). The upregulation of miR-2184 in the single M-cells facilitates axon regeneration, while the specific sponge-induced silencing of miR-2184 leads to impeded axon regeneration. We show that , a downstream target of miR-2184, negatively regulates axon regeneration, and the regeneration suppression by depends on its binding to Ca. Furthermore, pharmacological stimulation of the cAMP/PKA pathway suggests that changes in the readily releasable pool may affect axon regeneration. Our data indicate that miR-2184 promotes axon regeneration of M-cells within the CNS by modulating the downstream target , providing valuable insights into potential therapeutic strategies.

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来源期刊

Journal of Genetics and Genomics 生物-生化与分子生物学

CiteScore

8.20

自引率

3.40%

发文量

4756

审稿时长

14 days

期刊介绍： The Journal of Genetics and Genomics (JGG, formerly known as Acta Genetica Sinica ) is an international journal publishing peer-reviewed articles of novel and significant discoveries in the fields of genetics and genomics. Topics of particular interest include but are not limited to molecular genetics, developmental genetics, cytogenetics, epigenetics, medical genetics, population and evolutionary genetics, genomics and functional genomics as well as bioinformatics and computational biology.