{"title":"用于青光眼瓣膜表面工程的微环境调控双亲水涂层","authors":"Shimeng Zhang , Yejia Liu , Linhua Li , Binjian Wang , Zezhen Zhang , Shiyan Chen , Guanghong Zhang , Qiongjian Huang , Xiao Chen , Jiang Chen , Chao Qu","doi":"10.1016/j.actbio.2024.04.003","DOIUrl":null,"url":null,"abstract":"<div><p>Glaucoma valves (GVs) play an essential role in treating glaucoma. However, fibrosis after implantation has limited their long-term success in clinical applications. In this study, we aimed to develop a comprehensive surface-engineering strategy to improve the biocompatibility of GVs by constructing a microenvironment-regulated and dual-hydrophilic antifouling coating on a GV material (silicone rubber, SR). The coating was based on a superhydrophilic polydopamine (SPD) coating with good short-range superhydrophilicity and antifouling abilities. In addition, SPD coatings contain many phenolic hydroxyl groups that can effectively resist oxidative stress and the inflammatory microenvironment. Furthermore, based on its <em>in situ</em> photocatalytic free-radical polymerization properties, the SPD coating polymerized poly 2-methylacryloxyethylphosphocholine, providing an additional long-range hydrophilic and antifouling effect. The <em>in vitro</em> test results showed that the microenvironment-regulated and dual-hydrophilic coatings had anti-protein contamination, anti-oxidation, anti-inflammation, and anti-fiber proliferation capabilities. The <em>in vivo</em> test results indicated that this coating substantially reduced the fiber encapsulation formation of the SR material by inhibiting inflammation and fibrosis. This design strategy for dual hydrophilic coatings with microenvironmental regulation can provide a valuable reference for the surface engineering design of novel medical implantable devices.</p></div><div><h3>Statement of significance</h3><p>Superhydrophilic polydopamine (SPD) coatings were prepared on silicone rubber (SR) by a two-electron oxidation method. Introduction of pMPC to SPD surface using photocatalytic radical polymerization to obtain a dual-hydrophilic coating. The dual-hydrophilic coating effectively modulates the oxidative and inflammatory microenvironment. This coating significantly reduced protein contamination and adhesion of inflammatory cells and fibroblasts <em>in vitro</em>. The coating-modified SR inhibits inflammatory and fibrosis responses <em>in vivo</em>, promising to serve the glaucoma valves.</p></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":null,"pages":null},"PeriodicalIF":9.4000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microenvironment-regulated dual-hydrophilic coatings for glaucoma valve surface engineering\",\"authors\":\"Shimeng Zhang , Yejia Liu , Linhua Li , Binjian Wang , Zezhen Zhang , Shiyan Chen , Guanghong Zhang , Qiongjian Huang , Xiao Chen , Jiang Chen , Chao Qu\",\"doi\":\"10.1016/j.actbio.2024.04.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Glaucoma valves (GVs) play an essential role in treating glaucoma. However, fibrosis after implantation has limited their long-term success in clinical applications. In this study, we aimed to develop a comprehensive surface-engineering strategy to improve the biocompatibility of GVs by constructing a microenvironment-regulated and dual-hydrophilic antifouling coating on a GV material (silicone rubber, SR). The coating was based on a superhydrophilic polydopamine (SPD) coating with good short-range superhydrophilicity and antifouling abilities. In addition, SPD coatings contain many phenolic hydroxyl groups that can effectively resist oxidative stress and the inflammatory microenvironment. Furthermore, based on its <em>in situ</em> photocatalytic free-radical polymerization properties, the SPD coating polymerized poly 2-methylacryloxyethylphosphocholine, providing an additional long-range hydrophilic and antifouling effect. The <em>in vitro</em> test results showed that the microenvironment-regulated and dual-hydrophilic coatings had anti-protein contamination, anti-oxidation, anti-inflammation, and anti-fiber proliferation capabilities. The <em>in vivo</em> test results indicated that this coating substantially reduced the fiber encapsulation formation of the SR material by inhibiting inflammation and fibrosis. This design strategy for dual hydrophilic coatings with microenvironmental regulation can provide a valuable reference for the surface engineering design of novel medical implantable devices.</p></div><div><h3>Statement of significance</h3><p>Superhydrophilic polydopamine (SPD) coatings were prepared on silicone rubber (SR) by a two-electron oxidation method. Introduction of pMPC to SPD surface using photocatalytic radical polymerization to obtain a dual-hydrophilic coating. The dual-hydrophilic coating effectively modulates the oxidative and inflammatory microenvironment. This coating significantly reduced protein contamination and adhesion of inflammatory cells and fibroblasts <em>in vitro</em>. The coating-modified SR inhibits inflammatory and fibrosis responses <em>in vivo</em>, promising to serve the glaucoma valves.</p></div>\",\"PeriodicalId\":237,\"journal\":{\"name\":\"Acta Biomaterialia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.4000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Biomaterialia\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1742706124001764\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Biomaterialia","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1742706124001764","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
摘要
青光眼瓣膜(GVs)在治疗青光眼方面发挥着至关重要的作用。然而,植入后的纤维化限制了其在临床应用中的长期成功。在本研究中,我们旨在开发一种全面的表面工程策略,通过在青光眼瓣膜材料(硅橡胶,SR)上构建微环境调控和双亲水防污涂层来改善青光眼瓣膜的生物相容性。该涂层基于超亲水聚多巴胺(SPD)涂层,具有良好的短程超亲水性和防污能力。此外,SPD 涂层含有许多酚羟基,能有效抵抗氧化应激和炎症微环境。此外,基于其光催化自由基聚合特性,SPD 涂层聚合了聚 2-甲基丙烯酰氧乙基磷酸胆碱,提供了额外的长程亲水性和防污效果。测试结果表明,微环境调节型双亲水涂层具有抗蛋白质污染、抗氧化、抗炎症和抗纤维增殖的能力。测试结果表明,这种涂层通过抑制炎症和纤维化,大大减少了 SR 材料的纤维包裹形成。这种具有微环境调控功能的双亲水涂层设计策略可为新型医疗植入设备的表面工程设计提供有价值的参考。通过双电子氧化法在硅橡胶(SR)上制备了超亲水聚多巴胺(SPD)涂层。利用光催化自由基聚合将 pMPC 引入 SPD 表面,从而获得双亲水涂层。这种双重亲水涂层能有效调节氧化和炎症微环境。这种涂层大大减少了蛋白质污染以及炎症细胞和成纤维细胞的粘附。经涂层修饰的 SR 可抑制炎症和纤维化反应,有望用于青光眼瓣膜。
Microenvironment-regulated dual-hydrophilic coatings for glaucoma valve surface engineering
Glaucoma valves (GVs) play an essential role in treating glaucoma. However, fibrosis after implantation has limited their long-term success in clinical applications. In this study, we aimed to develop a comprehensive surface-engineering strategy to improve the biocompatibility of GVs by constructing a microenvironment-regulated and dual-hydrophilic antifouling coating on a GV material (silicone rubber, SR). The coating was based on a superhydrophilic polydopamine (SPD) coating with good short-range superhydrophilicity and antifouling abilities. In addition, SPD coatings contain many phenolic hydroxyl groups that can effectively resist oxidative stress and the inflammatory microenvironment. Furthermore, based on its in situ photocatalytic free-radical polymerization properties, the SPD coating polymerized poly 2-methylacryloxyethylphosphocholine, providing an additional long-range hydrophilic and antifouling effect. The in vitro test results showed that the microenvironment-regulated and dual-hydrophilic coatings had anti-protein contamination, anti-oxidation, anti-inflammation, and anti-fiber proliferation capabilities. The in vivo test results indicated that this coating substantially reduced the fiber encapsulation formation of the SR material by inhibiting inflammation and fibrosis. This design strategy for dual hydrophilic coatings with microenvironmental regulation can provide a valuable reference for the surface engineering design of novel medical implantable devices.
Statement of significance
Superhydrophilic polydopamine (SPD) coatings were prepared on silicone rubber (SR) by a two-electron oxidation method. Introduction of pMPC to SPD surface using photocatalytic radical polymerization to obtain a dual-hydrophilic coating. The dual-hydrophilic coating effectively modulates the oxidative and inflammatory microenvironment. This coating significantly reduced protein contamination and adhesion of inflammatory cells and fibroblasts in vitro. The coating-modified SR inhibits inflammatory and fibrosis responses in vivo, promising to serve the glaucoma valves.
期刊介绍:
Acta Biomaterialia is a monthly peer-reviewed scientific journal published by Elsevier. The journal was established in January 2005. The editor-in-chief is W.R. Wagner (University of Pittsburgh). The journal covers research in biomaterials science, including the interrelationship of biomaterial structure and function from macroscale to nanoscale. Topical coverage includes biomedical and biocompatible materials.