利用多重光谱探究环丙氟哌酸与人血清白蛋白的相互作用

IF 2.3 4区 化学 Q2 Agricultural and Biological Sciences
Qiao Pan, Chengfeng Yao, Yulin Zhu, Shujun Shang
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引用次数: 0

摘要

在体外模拟生理条件下的不同温度条件下,使用基于光谱的方法研究了环丙酚与人血清白蛋白(HSA)之间的相互作用。随着环丙酚浓度的增加,人血清白蛋白(HSA)的本征 Trp 荧光淬灭是该分析的驱动工具。实验结果证明,环丙酚通过静态淬灭机制淬灭了 HSA 的本征荧光。热力学参数(ΔG = -2.35 × 104 J-mol-1,ΔS = -131 J-mol-1-K-1,ΔH = -6.39 × 104 J-mol-1,310 K 时)、结合位点(n = 0.83)和结合常数(KA = 9.12 × 103 M-1)表明,氢键和范德华力在 HSA 与环丙酚的结合中起主要作用,结合力较弱。此外,圆二色性、同步和三维荧光光谱结果表明,HSA 在环丙酚存在下发生了适应性结构变化。此外,研究还考察了一些常见金属离子对环丙氟哌酸与 HSA 结合力的影响,结果表明 Fe3+ 和 Hg2+ 有助于延长环丙氟哌酸的储存时间并提高药效。该研究为阐明环丙氟哌酸与 HSA 的结合机制提供了准确、全面的基础数据,有助于了解环丙氟哌酸在血液运输过程中对蛋白质功能的影响以及在体内的活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Probing the interaction of ciprofol and human serum albumin using multiple spectroscopies

Probing the interaction of ciprofol and human serum albumin using multiple spectroscopies

The interaction between ciprofol and human serum albumin (HSA) was studied using spectroscopy-based approaches at different temperatures under simulated physiological conditions in vitro. Quenching of intrinsic Trp fluorescence of HSA with increasing ciprofol concentration is the actuating tool in the analysis. Experimental results proved that ciprofol quenched the intrinsic fluorescence of HSA through a static quenching mechanism. The thermodynamic parameters (ΔG = -2.35 × 104 J·mol−1, ΔS = -131 J·mol−1·K−1, and ΔH = -6.39 × 104 J·mol−1 at 310 K), binding sites (n = 0.83), and binding constant (KA = 9.12 × 103 M−1) indicated that hydrogen bond and van der Waals forces played a major role in the HSA-ciprofol association with weak binding force. Furthermore, the circular dichroism, synchronous, and three-dimensional fluorescence spectral results indicated adaptive structural changes of HSA in the presence of ciprofol. In addition, the effect of some common metal ions on the binding between ciprofol and HSA was examined, and Fe3+ and Hg2+ were proven to help prolong the storage time and improve the drug efficacy. The study provides accurate and full basic data for clarifying the binding mechanisms of ciprofol with HSA and helps understand its effect on protein function during the blood transportation process and activity in vivo.

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来源期刊
CiteScore
3.30
自引率
8.70%
发文量
0
审稿时长
3-8 weeks
期刊介绍: The Journal of Inclusion Phenomena and Macrocyclic Chemistry is the premier interdisciplinary publication reporting on original research into all aspects of host-guest systems. Examples of specific areas of interest are: the preparation and characterization of new hosts and new host-guest systems, especially those involving macrocyclic ligands; crystallographic, spectroscopic, thermodynamic and theoretical studies; applications in chromatography and inclusion polymerization; enzyme modelling; molecular recognition and catalysis by inclusion compounds; intercalates in biological and non-biological systems, cyclodextrin complexes and their applications in the agriculture, flavoring, food and pharmaceutical industries; synthesis, characterization and applications of zeolites. The journal publishes primarily reports of original research and preliminary communications, provided the latter represent a significant advance in the understanding of inclusion science. Critical reviews dealing with recent advances in the field are a periodic feature of the journal.
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