Jiali Feng , Xuefang Mu , Shuzhi Zhang , Xiaoyan Shen , Yang Zhou , Shufang Liu , Wen Cao
{"title":"一种新分离出来的耐卤 Rubrivivax gelatinosus MBE,能够在铵存在下产生氢气","authors":"Jiali Feng , Xuefang Mu , Shuzhi Zhang , Xiaoyan Shen , Yang Zhou , Shufang Liu , Wen Cao","doi":"10.1016/j.biombioe.2024.107214","DOIUrl":null,"url":null,"abstract":"<div><p>The large-scale cultivation of photosynthetic bacteria requires significant quantities of fresh water. Screening strains that can thrive in brackish water or seawater is a solution to mitigate the dwindling availability of freshwater. Through phylogenetic analysis based on 16S rDNA sequences, this study identified a strain <em>Rubrivivax gelatinosus</em> MBE isolated from seawater. The effects of several key factors, including carbon sources, pH, NH<sub>4</sub><sup>+</sup> and NaCl concentration on hydrogen production performance were investigated. <em>Rubrivivax gelatinosus</em> MBE showed a preference for glucose, but not organic acids for hydrogen production. It also exhibited alkalinity, achieving its peak hydrogen yield of 4626.82 ± 155.65 mL⋅L<sup>−1</sup> at pH 8. Interestingly, <em>Rubrivivax gelatinosus</em> MBE could exploit ammonium nitrogen for hydrogen synthesis, thriving even in specific ammonium and salt concentrations. Its resilience was further highlighted by a maximum hydrogen production yield of 4741.41 ± 167.01 mL⋅L<sup>−1</sup> and a hydrogen production rate of 37.59 ± 3.37 mL⋅L<sup>−1</sup>⋅h<sup>−1</sup> with 10 g⋅L<sup>−1</sup> NaCl, underscoring its significant salt tolerance. These results suggested that <em>Rubrivivax gelatinosus</em> MBE could be a promising candidate for biofuel production, and it is significant to take sustainability study on the application of MBE to deal with alkali wastewater from paper making, pharmacy and printing and high salinity wastewater in the future.</p></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":null,"pages":null},"PeriodicalIF":5.8000,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A newly isolated halotolerant Rubrivivax gelatinosus MBE with capable of producing hydrogen in the presence of ammonium\",\"authors\":\"Jiali Feng , Xuefang Mu , Shuzhi Zhang , Xiaoyan Shen , Yang Zhou , Shufang Liu , Wen Cao\",\"doi\":\"10.1016/j.biombioe.2024.107214\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The large-scale cultivation of photosynthetic bacteria requires significant quantities of fresh water. Screening strains that can thrive in brackish water or seawater is a solution to mitigate the dwindling availability of freshwater. Through phylogenetic analysis based on 16S rDNA sequences, this study identified a strain <em>Rubrivivax gelatinosus</em> MBE isolated from seawater. The effects of several key factors, including carbon sources, pH, NH<sub>4</sub><sup>+</sup> and NaCl concentration on hydrogen production performance were investigated. <em>Rubrivivax gelatinosus</em> MBE showed a preference for glucose, but not organic acids for hydrogen production. It also exhibited alkalinity, achieving its peak hydrogen yield of 4626.82 ± 155.65 mL⋅L<sup>−1</sup> at pH 8. Interestingly, <em>Rubrivivax gelatinosus</em> MBE could exploit ammonium nitrogen for hydrogen synthesis, thriving even in specific ammonium and salt concentrations. Its resilience was further highlighted by a maximum hydrogen production yield of 4741.41 ± 167.01 mL⋅L<sup>−1</sup> and a hydrogen production rate of 37.59 ± 3.37 mL⋅L<sup>−1</sup>⋅h<sup>−1</sup> with 10 g⋅L<sup>−1</sup> NaCl, underscoring its significant salt tolerance. These results suggested that <em>Rubrivivax gelatinosus</em> MBE could be a promising candidate for biofuel production, and it is significant to take sustainability study on the application of MBE to deal with alkali wastewater from paper making, pharmacy and printing and high salinity wastewater in the future.</p></div>\",\"PeriodicalId\":253,\"journal\":{\"name\":\"Biomass & Bioenergy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2024-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomass & Bioenergy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0961953424001673\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass & Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0961953424001673","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
A newly isolated halotolerant Rubrivivax gelatinosus MBE with capable of producing hydrogen in the presence of ammonium
The large-scale cultivation of photosynthetic bacteria requires significant quantities of fresh water. Screening strains that can thrive in brackish water or seawater is a solution to mitigate the dwindling availability of freshwater. Through phylogenetic analysis based on 16S rDNA sequences, this study identified a strain Rubrivivax gelatinosus MBE isolated from seawater. The effects of several key factors, including carbon sources, pH, NH4+ and NaCl concentration on hydrogen production performance were investigated. Rubrivivax gelatinosus MBE showed a preference for glucose, but not organic acids for hydrogen production. It also exhibited alkalinity, achieving its peak hydrogen yield of 4626.82 ± 155.65 mL⋅L−1 at pH 8. Interestingly, Rubrivivax gelatinosus MBE could exploit ammonium nitrogen for hydrogen synthesis, thriving even in specific ammonium and salt concentrations. Its resilience was further highlighted by a maximum hydrogen production yield of 4741.41 ± 167.01 mL⋅L−1 and a hydrogen production rate of 37.59 ± 3.37 mL⋅L−1⋅h−1 with 10 g⋅L−1 NaCl, underscoring its significant salt tolerance. These results suggested that Rubrivivax gelatinosus MBE could be a promising candidate for biofuel production, and it is significant to take sustainability study on the application of MBE to deal with alkali wastewater from paper making, pharmacy and printing and high salinity wastewater in the future.
期刊介绍:
Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials.
The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy.
Key areas covered by the journal:
• Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation.
• Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal.
• Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes
• Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation
• Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.