野樱桃(Maxim.)微繁殖的替代系统

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Daniela Sanson, Alexandre Techy de Almeida Garrett, Isy Cavalhães Rodrigues, Gabriel de Magalhães Miranda, Fabricio William de Ávila, Cristiane Alves Fogaça, Ezequiel Gasparin, Rogério Bobrowski, Fabiana Schmidt Bandeira Peres
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引用次数: 0

摘要

本文评估了野樱桃的体外繁殖方案。分析了 15.0、30.0 和 45.0 g L-1 蔗糖和三种烧瓶密封方法(聚氯乙烯薄膜、硬聚丙烯盖和带膜硬盖)在繁殖、生根和适应阶段的影响。在离体繁殖时,使用的是在木本植物培养基(WPM)中离体发芽的幼苗中长约 1 厘米、含有两对叶片的嫩梢。在 40、80 和 120 d 的三次移栽中,分别对嫩枝顶端的成活率、嫩枝数量、嫩枝长度、叶片数量和繁殖率进行评估。在生根阶段,评估生根率、根数和根长。在遮荫和全日照条件下的适应阶段,评估了幼苗的存活率和生物特征,包括茎直径、高度和叶片数。在浓度为 45.0 g L-1 的培养基中添加蔗糖,并用 PVC 塑料薄膜密封,结果显示对野马花属植物的体外繁殖效果不佳。在培养基中添加浓度为 15.0 至 30.0 g L-1 的蔗糖有利于离体生根。从生根率和根长的角度来看,使用透气膜进行密封能使幼苗生长得更旺盛,这些幼苗在适应阶段表现突出,成活率更高,生物特征也得到改善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Alternative system for micropropagation of Prunus campanulata (Maxim.)

Alternative system for micropropagation of Prunus campanulata (Maxim.)

Herein, an in vitro multiplication protocol for Prunus campanulata was evaluated. The effects of 15.0, 30.0, and 45.0 g L−1 sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For in vitro multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated in vitro in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L−1, associated with sealing using PVC plastic film, showed inadequate results for in vitro multiplication of P. campanulata. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L−1 favored in vitro rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics.

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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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