Ah-In Yang, Bora Kim, Sung-Hong Joe, Hae-In Joe, Hanna Choe, Ki Hyun Kim, Min Ok Jun, Na-Ri Shin
{"title":"从韩国传统发酵海鲜 Jeotgal 中分离出的 Sporosarcina jeotgali sp.","authors":"Ah-In Yang, Bora Kim, Sung-Hong Joe, Hae-In Joe, Hanna Choe, Ki Hyun Kim, Min Ok Jun, Na-Ri Shin","doi":"10.1007/s12275-024-00106-3","DOIUrl":null,"url":null,"abstract":"<p>Three novel, Gram-stain-positive, obligate aerobic, catalase- and oxidase-positive bacterial strains, designated B2O-1<sup>T</sup>, T2O-4<sup>T</sup>, and 0.2-SM1T-5<sup>T</sup>, were isolated from jeotgal, a traditional Korean fermented seafood. Strains B2O-1<sup>T</sup>, T2O-4<sup>T</sup>, and 0.2-SM1T-5<sup>T</sup> exhibited distinct colony colors, characterized by pink, yellow, and red opaque circular colonies, respectively. Phylogenetic analysis revealed that three strains formed a paraphyletic clade within the genus <i>Sporosarcina</i> and shared < 99.0% similarity with <i>Sporosarcina aquimarina</i> KCTC 3840<sup>T</sup> and <i>Sporosarcina saromensis</i> KCTC 13119<sup>T</sup> in their 16S rRNA gene sequences. The three strains exhibiting Orthologous Average Nucleotide Identity values < 79.3% and digital DNA-DNA hybridization values < 23.1% within the genus <i>Sporosarcina</i> affirmed their distinctiveness. Strains B2O-1<sup>T</sup>, T2O-4<sup>T</sup>, and 0.2-SM1T-5<sup>T</sup> contained MK-7 as a sole respiratory menaquinone and A4<i>α</i> type peptidoglycan based on lysine with alanine, glutamic acid, and aspartic acid. The common polar lipids include diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Strain T2O-4<sup>T</sup> contained one unidentified phospholipid, whereas strain 0.2-SM1T-5<sup>T</sup> contained two unidentified phospholipids. Cellular fatty acid profiles, with C<sub>15:0</sub> anteiso as the major fatty acid, supported the affiliation of the three strains to the genus <i>Sporosarcina</i>. Based on the polyphasic characteristics, strains B2O-1<sup>T</sup> (= KCTC 43506<sup>T</sup> = JCM 36032<sup>T</sup>), T2O-4<sup>T</sup> (= KCTC 43489<sup>T</sup> = JCM 36031<sup>T</sup>), and 0.2-SM1T-5<sup>T</sup> (= KCTC 43519<sup>T</sup> = JCM 36034<sup>T</sup>) represent three novel species within the genus <i>Sporosarcina</i>, named <i>Sporosarcina jeotgali</i> sp. nov., <i>Sporosarcina oncorhynchi</i> sp. nov., and <i>Sporosarcina trichiuri</i> sp. nov., respectively.</p>","PeriodicalId":16546,"journal":{"name":"Journal of Microbiology","volume":"13 1","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sporosarcina jeotgali sp. nov., Sporosarcina oncorhynchi sp. nov., and Sporosarcina trichiuri sp. nov., Isolated from Jeotgal, a Traditional Korean Fermented Seafood\",\"authors\":\"Ah-In Yang, Bora Kim, Sung-Hong Joe, Hae-In Joe, Hanna Choe, Ki Hyun Kim, Min Ok Jun, Na-Ri Shin\",\"doi\":\"10.1007/s12275-024-00106-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Three novel, Gram-stain-positive, obligate aerobic, catalase- and oxidase-positive bacterial strains, designated B2O-1<sup>T</sup>, T2O-4<sup>T</sup>, and 0.2-SM1T-5<sup>T</sup>, were isolated from jeotgal, a traditional Korean fermented seafood. Strains B2O-1<sup>T</sup>, T2O-4<sup>T</sup>, and 0.2-SM1T-5<sup>T</sup> exhibited distinct colony colors, characterized by pink, yellow, and red opaque circular colonies, respectively. Phylogenetic analysis revealed that three strains formed a paraphyletic clade within the genus <i>Sporosarcina</i> and shared < 99.0% similarity with <i>Sporosarcina aquimarina</i> KCTC 3840<sup>T</sup> and <i>Sporosarcina saromensis</i> KCTC 13119<sup>T</sup> in their 16S rRNA gene sequences. The three strains exhibiting Orthologous Average Nucleotide Identity values < 79.3% and digital DNA-DNA hybridization values < 23.1% within the genus <i>Sporosarcina</i> affirmed their distinctiveness. Strains B2O-1<sup>T</sup>, T2O-4<sup>T</sup>, and 0.2-SM1T-5<sup>T</sup> contained MK-7 as a sole respiratory menaquinone and A4<i>α</i> type peptidoglycan based on lysine with alanine, glutamic acid, and aspartic acid. The common polar lipids include diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Strain T2O-4<sup>T</sup> contained one unidentified phospholipid, whereas strain 0.2-SM1T-5<sup>T</sup> contained two unidentified phospholipids. Cellular fatty acid profiles, with C<sub>15:0</sub> anteiso as the major fatty acid, supported the affiliation of the three strains to the genus <i>Sporosarcina</i>. Based on the polyphasic characteristics, strains B2O-1<sup>T</sup> (= KCTC 43506<sup>T</sup> = JCM 36032<sup>T</sup>), T2O-4<sup>T</sup> (= KCTC 43489<sup>T</sup> = JCM 36031<sup>T</sup>), and 0.2-SM1T-5<sup>T</sup> (= KCTC 43519<sup>T</sup> = JCM 36034<sup>T</sup>) represent three novel species within the genus <i>Sporosarcina</i>, named <i>Sporosarcina jeotgali</i> sp. nov., <i>Sporosarcina oncorhynchi</i> sp. nov., and <i>Sporosarcina trichiuri</i> sp. nov., respectively.</p>\",\"PeriodicalId\":16546,\"journal\":{\"name\":\"Journal of Microbiology\",\"volume\":\"13 1\",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12275-024-00106-3\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12275-024-00106-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Sporosarcina jeotgali sp. nov., Sporosarcina oncorhynchi sp. nov., and Sporosarcina trichiuri sp. nov., Isolated from Jeotgal, a Traditional Korean Fermented Seafood
Three novel, Gram-stain-positive, obligate aerobic, catalase- and oxidase-positive bacterial strains, designated B2O-1T, T2O-4T, and 0.2-SM1T-5T, were isolated from jeotgal, a traditional Korean fermented seafood. Strains B2O-1T, T2O-4T, and 0.2-SM1T-5T exhibited distinct colony colors, characterized by pink, yellow, and red opaque circular colonies, respectively. Phylogenetic analysis revealed that three strains formed a paraphyletic clade within the genus Sporosarcina and shared < 99.0% similarity with Sporosarcina aquimarina KCTC 3840T and Sporosarcina saromensis KCTC 13119T in their 16S rRNA gene sequences. The three strains exhibiting Orthologous Average Nucleotide Identity values < 79.3% and digital DNA-DNA hybridization values < 23.1% within the genus Sporosarcina affirmed their distinctiveness. Strains B2O-1T, T2O-4T, and 0.2-SM1T-5T contained MK-7 as a sole respiratory menaquinone and A4α type peptidoglycan based on lysine with alanine, glutamic acid, and aspartic acid. The common polar lipids include diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Strain T2O-4T contained one unidentified phospholipid, whereas strain 0.2-SM1T-5T contained two unidentified phospholipids. Cellular fatty acid profiles, with C15:0 anteiso as the major fatty acid, supported the affiliation of the three strains to the genus Sporosarcina. Based on the polyphasic characteristics, strains B2O-1T (= KCTC 43506T = JCM 36032T), T2O-4T (= KCTC 43489T = JCM 36031T), and 0.2-SM1T-5T (= KCTC 43519T = JCM 36034T) represent three novel species within the genus Sporosarcina, named Sporosarcina jeotgali sp. nov., Sporosarcina oncorhynchi sp. nov., and Sporosarcina trichiuri sp. nov., respectively.
期刊介绍:
Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.