家禽粪便土壤改良剂对耐抗生素大肠杆菌的影响

IF 2.2 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Getahun E. Agga, Lisa M. Durso, Karamat R. Sistani
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引用次数: 0

摘要

鉴于矿物肥料成本高且不可再生,人们对创新使用粪肥材料(如家禽粪便)越来越感兴趣。然而,粪肥既增加了土壤中的养分,也增加了土壤中的微生物,包括抗生素耐药大肠杆菌(AREc)。在一项随机田间试验(2017 年 5 月至 10 月)中,评估了 PL 土壤改良对玉米田中 AREc 的影响。两种冬季耕作制度(休耕和覆盖作物)被分配到整个地块,三种春季施肥处理(未处理对照 [UC]、PL 和商业肥料 [CF])被分配到子地块。在施肥后的第 0 天、第 7 天、第 28 天、第 70 天、第 98 天和第 172 天采集 0 至 15 厘米的土壤。对样本进行培养,以确定普通、耐四环素(TETr)、耐第三代头孢菌素(3GCR)大肠杆菌分离物以及产广谱β-内酰胺酶(ESBL)肠杆菌科细菌的计数和流行率。与 UC 或 CF 相比,PL 土壤改良剂在第 7 天和第 28 天明显增加了普通大肠杆菌、TETr 大肠杆菌和 3GCr 大肠杆菌的水平(p < 0.05)。第 28 天后,不同肥料处理的 AREc 没有明显差异(p > 0.05),并在第 70 天恢复到基线水平。在 16 个样本中检测到了产 ESBL 的肠杆菌科细菌,其中大部分是在第 70 天检测到的。与未覆盖作物相比,覆盖作物在第 28 天明显降低了 TETr 大肠杆菌的浓度,但对 3GCr 大肠杆菌和产 ESBL 肠杆菌科细菌的流行率没有明显影响。通过聚合酶链式反应,所有产 ESBL 的肠杆菌科细菌和 79% 的 3GCr 大肠杆菌分离物的 blaCTX-M 基因均呈阳性。结果表明,与矿物肥料相比,PL 土壤改良剂能短暂提高 AREc 的水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of poultry litter soil amendment on antibiotic-resistant Escherichia coli

Effect of poultry litter soil amendment on antibiotic-resistant Escherichia coli

Given the high cost and non-renewability of mineral-based fertilizers, there is increasing interest in the innovative use of manure-based materials, such as poultry litter (PL). However, manure-based fertilizers add both nutrients and microbes to the soil, including antibiotic-resistant Escherichia coli (AREc). PL soil amendment impact on AREc in corn fields was evaluated in a randomized field experiment (May–October 2017). Two winter cropping systems (fallow and cover crop) were assigned to whole plots, with three spring-applied fertilizer treatments (untreated control [UC], PL, and commercial fertilizer [CF]) assigned to subplots. Soil was collected from 0 to 15 cm on days 0, 7, 28, 70, 98, and 172 post-treatment applications. Samples were cultured for the enumeration and prevalence of generic, tetracycline-resistant (TETr), third-generation cephalosporin-resistant (3GCr) E. coli isolates, and extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. PL soil amendment significantly (p < 0.05) increased the levels of generic E. coli, TETr E. coli, and 3GCr E. coli on days 7 and 28 compared to UC or CF. Beyond day 28, AREc did not significantly (> 0.05) differ by fertilizer treatment and returned to baseline on day 70. ESBL-producing Enterobacteriaceae were detected from 16 samples, mostly on day 70. Cover crop significantly decreased TETr E. coli concentration on day 28, with no significant effects on the prevalence of 3GCr E. coli and ESBL-producing Enterobacteriaceae compared to no cover crop. All ESBL-producing Enterobacteriaceae and 79% of the 3GCr E. coli isolates were positive for blaCTX-M gene by polymerase chain reaction. Results show that PL soil amendment transiently increases the levels of AREc compared to mineral fertilizer.

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来源期刊
Journal of environmental quality
Journal of environmental quality 环境科学-环境科学
CiteScore
4.90
自引率
8.30%
发文量
123
审稿时长
3 months
期刊介绍: Articles in JEQ cover various aspects of anthropogenic impacts on the environment, including agricultural, terrestrial, atmospheric, and aquatic systems, with emphasis on the understanding of underlying processes. To be acceptable for consideration in JEQ, a manuscript must make a significant contribution to the advancement of knowledge or toward a better understanding of existing concepts. The study should define principles of broad applicability, be related to problems over a sizable geographic area, or be of potential interest to a representative number of scientists. Emphasis is given to the understanding of underlying processes rather than to monitoring. Contributions are accepted from all disciplines for consideration by the editorial board. Manuscripts may be volunteered, invited, or coordinated as a special section or symposium.
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