Martha Ilett, Maryam Afzali, Bilal Abdulkarim, Zabeada Aslam, Stephanie Foster, Miguel Burgos-Ruiz, Yi-Yeoun Kim, Fiona C. Meldrum, Rik M. Drummond-Brydson
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引用次数: 0
摘要
我们比较了使用扫描电子显微镜和透射电子显微镜（SEM 和 TEM）研究溶液结晶过程的常用电子显微镜样品制备方法。我们重点研究了两种被广泛研究的无机体系：硫酸钙、石膏（CaSO4-2H2O）和碳酸钙（CaCO3）。我们发现不同的样品制备方法在结晶动力学和多晶体选择方面存在明显差异，这表明干燥和化学淬火会产生严重的伪影，从而掩盖结晶溶液的真实原生状态。总之，这些结果凸显了低温淬火结晶溶液的重要性，以及使用全低温 TEM 作为研究结晶早期阶段最可靠方法的重要性。
Studying crystallisation processes using electron microscopy: The importance of sample preparation
We present a comparison of common electron microscopy sample preparation methods for studying crystallisation processes from solution using both scanning and transmission electron microscopy (SEM and TEM). We focus on two widely studied inorganic systems: calcium sulphate, gypsum (CaSO4·2H2O) and calcium carbonate (CaCO3). We find significant differences in crystallisation kinetics and polymorph selection between the different sample preparation methods, which indicate that drying and chemical quenching can induce severe artefacts that are capable of masking the true native state of the crystallising solution. Overall, these results highlight the importance of cryogenic (cryo)-quenching crystallising solutions and the use of full cryo-TEM as the most reliable method for studying the early stages of crystallisation.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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