比较小鼠感染或免疫六种毛线虫后对碳水化合物的 IgG3 反应

IF 1.3 3区 生物学 Q4 PARASITOLOGY
M.A. Dea-Ayuela, A.R. Martinez-Fernandez, F. Bolas-Fernandez
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引用次数: 0

摘要

比较了感染或免疫了六种旋毛虫的 BALB/c 小鼠对碳水化合物表位的 IgG3 抗体反应:T.spiralis(T1)、T. nativa(T2)、T. britovi(T3)、T6、T. nelsoni(T7)和T8。使用同源分离物制备的糖基化和脱糖基化幼虫粗提取物(LCE),通过酶联免疫吸附试验(ELISA)和免疫印迹试验(Western blot)分别测定了感染或免疫后 IgG3 反应和抗原识别的动态。在所有物种中都发现了高度的蛋白质糖基化,并且具有相似的特征。只有在 T1 和 T6 分离物的 LCE 中才能完全实现脱糖基化。感染或免疫后 IgG3 反应的动态差异很大,而抗原识别特征似乎相似。不同物种的 IgG3 水平和抗原识别模式存在明显差异。在感染 T8 分离物时,IgG3 水平最高,而在感染 T6 分离物时,IgG3 水平最低。抗原脱糖后,不同种类的旋毛虫的 IgG3 反应明显降低或消失,识别模式明显改变或被抑制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of IgG3 responses to carbohydrates following mouse infection or immunization with six species of Trichinella
The IgG3 antibody responses to carbohydrate epitopes were compared in BALB/c mice infected or immunized with six species of Trichinella: T. spiralis (T1), T. nativa (T2), T. britovi (T3), T6, T. nelsoni (T7), and T8. The dynamics of IgG3 responses and antigen recognition following infection or immunization were measured by ELISA and Western blot respectively, using glycosylated and deglycosylated larval crude extracts (LCE) prepared from homologous isolates. A high degree of protein glycosylation was found in all species and with similar profiles. Deglycosylation was completely achieved only in LCE from T1 and T6 isolates. The dynamics of IgG3 responses following infection or immunization significantly differed whereas the antigen recognition profiles appeared similar. Variations in the levels and antigen recognition patterns of IgG3 among the different species were apparent. The highest IgG3 levels were recorded in infections by the T8 isolate and the lowest in infections by the T6 isolate, whereas for immunization the highest IgG3 response was induced by T7 and the lowest by T8. Following antigen deglycosylation, the IgG3 responses were significantly reduced or abrogated and the recognition patterns markedly modified or suppressed in the different species of Trichinella.
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来源期刊
Journal of Helminthology
Journal of Helminthology 生物-动物学
CiteScore
2.80
自引率
12.50%
发文量
127
审稿时长
3 months
期刊介绍: Journal of Helminthology publishes original papers and review articles on all aspects of pure and applied helminthology, particularly those helminth parasites of environmental health, medical or veterinary importance. Research papers on helminths in wildlife hosts, including plant and insect parasites, are also published along with taxonomic papers contributing to the systematics of a group. The journal will be of interest to academics and researchers involved in the fields of human and veterinary parasitology, public health, microbiology, ecology and biochemistry.
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