lgi-miR-2d通过靶向马尼拉蛤蜊(Ruditapes philippinarum)体内的mitf,可能参与贝壳黑色素的合成

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Qiaoyue Xu, Hongtao Nie, Qianying Ma, Jiadi Wang, Zhongming Huo, Xiwu Yan
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引用次数: 0

摘要

壳色作为重要的经济性状,也是育种和生产的关键目标性状。微RNA(miRNA)是一种内源性小非编码RNA,可转录后调控靶基因的表达,在贝壳颜色、应激反应、疾病性状等多种生命活动和生理过程中发挥重要作用。本研究探讨了lgi-miR-2d在马尼拉蛤Ruditapes philippinarum贝壳黑色素形成过程中的功能,以及lgi-miR-2d和靶基因Rpmitf的表达模式。我们进一步探讨和验证了Rpmitf与lgi-miR-2d之间的关系,并分别通过RNAi和注射lgi-miR-2d的抗凝血酶鉴定了贝壳颜色相关基因的表达水平变化。结果表明,lgi-miR-2d antagomir影响了其靶基因Rpmitf的表达。此外,为了证实 lgi-miR-2d 与 Rpmitf 之间的直接相互作用,我们还进行了双荧光素酶报告实验。结果显示,注射 Rpmitf dsRNA 后,与空白对照组相比,阳性处理组 Rpmitf 和 tyr 等黑色素相关基因的表达水平明显下降,表明 Rpmitf 在黑色素合成途径中起着关键作用。综上所述,我们推测lgi-miR-2d可能会对Rpmitf产生负调控作用,而Rpmitf可能会调控其他壳色相关基因,从而影响菲利宾鱼黑色素的合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum

The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum

Shell color as an important economic trait is also the crucial target trait for breeding and production. MicroRNA (miRNA) is an endogenous small non-coding RNA that can post-transcriptionally regulate the expression of target genes, it plays important roles in many life activities and physiological processes, such as shell color, stress response, and disease traits. In this study, we investigated the function of lgi-miR-2d in shell melanin formation and the expression patterns of lgi-miR-2d and target gene Rpmitf in Manila clam Ruditapes philippinarum. We further explored and verified the relationship between Rpmitf and lgi-miR-2d and identified the expression level of shell color-related gene changes by RNAi and injecting the antagomir of lgi-miR-2d, respectively. Our results indicated that lgi-miR-2d antagomir affected the expression of its target gene Rpmitf. In addition, the dual-luciferase reporter assay was conducted to confirm the direct interaction between lgi-miR-2d and Rpmitf. The results showed that the expression levels of melanin-related genes such as Rpmitf and tyr were significantly decreased in the positive treatment group compared with the blank control group after the Rpmitf dsRNA injection, indicating Rpmitf plays a crucial role in the melanin synthesis pathway. Taken together, we speculated that lgi-miR-2d might be negatively modulating Rpmitf, which might regulate other shell color-related genes, thereby affecting melanin synthesis in R. philippinarum.

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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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