组蛋白 H2B 的单泛素化是记忆形成过程中转录组的关键调节因子

IF 1.8 4区医学 Q4 NEUROSCIENCES

Learning & memory Pub Date : 2024-03-01 DOI:10.1101/lm.053912.123

Shaghayegh Navabpour, Kayla Farrell, Shannon E. Kincaid, Nour Omar, Madeline Musaus, Yu Lin, Hehuang Xie, Timothy J. Jarome

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引用次数: 0

摘要

组蛋白的翻译后修饰对记忆的形成至关重要。最近，我们发现组蛋白 H2B 在赖氨酸 120 处的单泛素化（H2Bub）对海马的记忆形成至关重要。然而，由 H2Bub 控制的转录组仍然未知。在这里，我们发现雄性大鼠的恐惧条件反射会增加或减少海马中 86 个基因的表达，但令人惊讶的是，siRNA 介导的 H2Bub 连接酶 Rnf20 的敲除会消除所有这些基因的变化，只有一个基因除外。这些发现表明，组蛋白 H2B 的单泛素化是记忆形成过程中转录组的一个关键调节因子。

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Monoubiquitination of histone H2B is a crucial regulator of the transcriptome during memory formation

Posttranslational modification of histone proteins is critical for memory formation. Recently, we showed that monoubiquitination of histone H2B at lysine 120 (H2Bub) is critical for memory formation in the hippocampus. However, the transcriptome controlled by H2Bub remains unknown. Here, we found that fear conditioning in male rats increased or decreased the expression of 86 genes in the hippocampus but, surprisingly, siRNA-mediated knockdown of the H2Bub ligase, Rnf20, abolished changes in all but one of these genes. These findings suggest that monoubiquitination of histone H2B is a crucial regulator of the transcriptome during memory formation.

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来源期刊

Learning & memory 医学-神经科学

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The neurobiology of learning and memory is entering a new interdisciplinary era. Advances in neuropsychology have identified regions of brain tissue that are critical for certain types of function. Electrophysiological techniques have revealed behavioral correlates of neuronal activity. Studies of synaptic plasticity suggest that some mechanisms of memory formation may resemble those of neural development. And molecular approaches have identified genes with patterns of expression that influence behavior. It is clear that future progress depends on interdisciplinary investigations. The current literature of learning and memory is large but fragmented. Until now, there has been no single journal devoted to this area of study and no dominant journal that demands attention by serious workers in the area, regardless of specialty. Learning & Memory provides a forum for these investigations in the form of research papers and review articles.