将表面工程重组大肠杆菌应用于工业电池废液中的锂回收

IF 3.2 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-04-04 DOI:10.1093/jimb/kuae012

Jaehoon Jeong, Vidhya Selvamani, Murali kannan Maruthamuthu, Kulandaisamy Arulsamy, Soon Ho Hong

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引用次数: 0

摘要

通过采用细菌细胞表面展示策略，设计了大肠杆菌来选择性地吸附和回收环境中的锂。锂结合肽 LBP1 被整合到大肠杆菌膜蛋白 OmpC 中。评估了环境条件对重组菌株吸附锂的影响，并通过 FE-SEM 和 XRD 分析了细胞表面的锂颗粒。为了提高锂的吸附能力，研究人员构建了二聚、三聚和四聚重复的 LBP1 肽，并将其展示在大肠杆菌表面。将构建的显示 LBP1 三聚体的重组大肠杆菌用于实际工业锂电池废水中回收锂。

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Application of the surface engineered recombinant Escherichia coli to the industrial battery waste solution for lithium recovery

Escherichia coli were engineered to selectively adsorb and recover lithium from the environment by employing bacterial cell surface display strategy. Lithium binding peptide LBP1 was integrated to the Escherichia coli membrane protein OmpC. The effect of environmental conditions on the adsorption of lithium by recombinant strain was evaluated, and lithium particles on cellular surface was analysed by FE-SEM and XRD. To elevate the lithium adsorption, dimeric, trimeric and tetrameric repeats of the LBP1 peptide was constructed and displayed on the surface of E. coli. The constructed recombinant E. coli displaying LBP1 trimer was applied to real industrial lithium battery wastewater to recover lithium.

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来源期刊

Journal of Industrial Microbiology & Biotechnology 工程技术-生物工程与应用微生物

CiteScore

7.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： The Journal of Industrial Microbiology and Biotechnology is an international journal which publishes papers describing original research, short communications, and critical reviews in the fields of biotechnology, fermentation and cell culture, biocatalysis, environmental microbiology, natural products discovery and biosynthesis, marine natural products, metabolic engineering, genomics, bioinformatics, food microbiology, and other areas of applied microbiology