Heba A. Hekal, Maggie E. Amer, Maher Amer, Mohamed A. El-Missiry, Azza I. Othman
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Similarly, treatment with Se resulted in downregulation of inflammatory cytokines (TNF-α and IL-6) while increasing IL-10 in serum of tumor-bearing mice. Conversely, selenium increased TNF- α and IL-6 and decreased IL-10 in tumor suggesting disruption of tumor immunity. The increased oxidative stress and inflammation in tumor tissue dysregulated cell cycle phases with increase apoptotic tumor cells population in G<sub>0</sub>/G<sub>1</sub> phase. This is supported by the increased levels apoptotic regulating proteins (Bax and caspase-3 and P-53) while decreasing Bcl-2 in the tumor tissue. Treatment with Se also resulted in increased comet parameters indicating DNA damage of tumor cells. Histopathological examination revealed a significant decrease in a number of neoplastic cells within tumor of mice that treated with Se. 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引用次数: 0
摘要
硒(Se)是癌症治疗中一种重要的微量营养生物大分子。本研究评估了 Se 的抗癌作用及其改善皮下实体艾氏癌小鼠健康状况的能力。实验共分四组,每组五只雌性 BALB/c 小鼠。其中两组在接受或不接受 Se 治疗的情况下移植艾氏肿瘤细胞。其他组作为对照组,接受或不接受 Se 治疗。Se 治疗显著减少了肿瘤小鼠的肿瘤体积和动物体重。Se 治疗显著增加了肿瘤中的氧化应激,同时改善了肿瘤小鼠血清中的氧化应激。同样,用 Se 治疗会导致肿瘤小鼠血清中炎症细胞因子(TNF-α 和 IL-6)的下调,同时增加 IL-10。相反,硒增加了肿瘤中的 TNF-α 和 IL-6,减少了 IL-10,这表明肿瘤免疫受到破坏。肿瘤组织中氧化应激和炎症的增加导致细胞周期阶段失调,G0/G1 期凋亡肿瘤细胞数量增加。肿瘤组织中的凋亡调节蛋白(Bax、caspase-3 和 P-53)水平升高,而 Bcl-2 水平下降,也证明了这一点。用 Se 处理还导致彗星参数增加,表明肿瘤细胞的 DNA 受到破坏。组织病理学检查显示,用 Se 治疗的小鼠肿瘤内的肿瘤细胞数量明显减少。总之,这些研究结果表明,Se疗法能显著抑制实体瘤的增殖和生长,同时缓解肿瘤小鼠的健康状况。
Selenium suppressed growth of Ehrlich solid tumor and improved health of tumor-bearing mice
Selenium (Se) is an important micronutritional biomolecule in cancer therapy. The current work evaluated the anticancer effect of Se and its ability to improve health of mice with solid Ehrlich carcinoma implanted subcutaneously. Four groups of five female BALB/c mice each were assembled. Ehrlich tumor cells were engrafted into two of them, either with or without Se therapy. The other groups served as control groups, either with or without Se treatment. Se treatment resulted in a notable decrease in both tumor volume and animal body mass in tumor-bearing mice. Treatment with Se markedly increased oxidative stress in tumor while ameliorating oxidative stress in sera of tumors-bearing mice. Similarly, treatment with Se resulted in downregulation of inflammatory cytokines (TNF-α and IL-6) while increasing IL-10 in serum of tumor-bearing mice. Conversely, selenium increased TNF- α and IL-6 and decreased IL-10 in tumor suggesting disruption of tumor immunity. The increased oxidative stress and inflammation in tumor tissue dysregulated cell cycle phases with increase apoptotic tumor cells population in G0/G1 phase. This is supported by the increased levels apoptotic regulating proteins (Bax and caspase-3 and P-53) while decreasing Bcl-2 in the tumor tissue. Treatment with Se also resulted in increased comet parameters indicating DNA damage of tumor cells. Histopathological examination revealed a significant decrease in a number of neoplastic cells within tumor of mice that treated with Se. In conclusion, these findings suggest that Se therapy significantly suppressed solid tumor proliferation and growth while mitigating the health status of tumor-bearing mice.
期刊介绍:
The Journal of Experimental Zoology – A publishes articles at the interface between Development, Physiology, Ecology and Evolution. Contributions that help to reveal how molecular, functional and ecological variation relate to one another are particularly welcome. The Journal publishes original research in the form of rapid communications or regular research articles, as well as perspectives and reviews on topics pertaining to the scope of the Journal. Acceptable articles are limited to studies on animals.