{"title":"通过与酵母菌共同培养改善绿藻衣藻的细胞生长","authors":"Yukino Karitani, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino","doi":"10.1007/s10529-024-03483-2","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Purpose</h3><p>CO<sub>2</sub> fixation methods using green algae have attracted considerable attention because they can be applied for the fixation of dilute CO<sub>2</sub> in the atmosphere. However, green algae generally exhibit low CO<sub>2</sub> fixation efficiency under atmospheric conditions. Therefore, it is a challenge to improve the CO<sub>2</sub> fixation efficiency of green algae under atmospheric conditions. Co-cultivation of certain microalgae with heterotrophic microorganisms can increase the growth potential of microalgae under atmospheric conditions. The objective of this study was to determine the culture conditions under which the growth potential of green algae <i>Chlamydomonas reinhardtii</i> is enhanced by co-culturing with the yeast <i>Saccharomyces cerevisiae</i>, and to identify the cause of the enhanced growth potential.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>When <i>C. reinhardtii</i> and <i>S. cerevisiae</i> were co-cultured with an initial green algae to yeast inoculum ratio of 1:3, the cell concentration of <i>C. reinhardtii</i> reached 133 × 10<sup>5</sup> cells/mL on day 18 of culture, which was 1.5 times higher than that of the monoculture. Transcriptome analysis revealed that the expression levels of 363 green algae and 815 yeast genes were altered through co-cultivation. These included genes responsible for ammonium transport and CO<sub>2</sub> enrichment mechanism in green algae and the genes responsible for glycolysis and stress responses in yeast.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>We successfully increased <i>C. reinhardtii</i> growth potential by co-culturing it with <i>S. cerevisiae</i>. The main reasons for this are likely to be an increase in inorganic nitrogen available to green algae via yeast metabolism and an increase in energy available for green algae growth instead of CO<sub>2</sub> enrichment.</p>","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae\",\"authors\":\"Yukino Karitani, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino\",\"doi\":\"10.1007/s10529-024-03483-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Purpose</h3><p>CO<sub>2</sub> fixation methods using green algae have attracted considerable attention because they can be applied for the fixation of dilute CO<sub>2</sub> in the atmosphere. However, green algae generally exhibit low CO<sub>2</sub> fixation efficiency under atmospheric conditions. Therefore, it is a challenge to improve the CO<sub>2</sub> fixation efficiency of green algae under atmospheric conditions. Co-cultivation of certain microalgae with heterotrophic microorganisms can increase the growth potential of microalgae under atmospheric conditions. The objective of this study was to determine the culture conditions under which the growth potential of green algae <i>Chlamydomonas reinhardtii</i> is enhanced by co-culturing with the yeast <i>Saccharomyces cerevisiae</i>, and to identify the cause of the enhanced growth potential.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>When <i>C. reinhardtii</i> and <i>S. cerevisiae</i> were co-cultured with an initial green algae to yeast inoculum ratio of 1:3, the cell concentration of <i>C. reinhardtii</i> reached 133 × 10<sup>5</sup> cells/mL on day 18 of culture, which was 1.5 times higher than that of the monoculture. Transcriptome analysis revealed that the expression levels of 363 green algae and 815 yeast genes were altered through co-cultivation. These included genes responsible for ammonium transport and CO<sub>2</sub> enrichment mechanism in green algae and the genes responsible for glycolysis and stress responses in yeast.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusion</h3><p>We successfully increased <i>C. reinhardtii</i> growth potential by co-culturing it with <i>S. cerevisiae</i>. The main reasons for this are likely to be an increase in inorganic nitrogen available to green algae via yeast metabolism and an increase in energy available for green algae growth instead of CO<sub>2</sub> enrichment.</p>\",\"PeriodicalId\":8929,\"journal\":{\"name\":\"Biotechnology Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-04-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology Letters\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s10529-024-03483-2\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Letters","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10529-024-03483-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae
Purpose
CO2 fixation methods using green algae have attracted considerable attention because they can be applied for the fixation of dilute CO2 in the atmosphere. However, green algae generally exhibit low CO2 fixation efficiency under atmospheric conditions. Therefore, it is a challenge to improve the CO2 fixation efficiency of green algae under atmospheric conditions. Co-cultivation of certain microalgae with heterotrophic microorganisms can increase the growth potential of microalgae under atmospheric conditions. The objective of this study was to determine the culture conditions under which the growth potential of green algae Chlamydomonas reinhardtii is enhanced by co-culturing with the yeast Saccharomyces cerevisiae, and to identify the cause of the enhanced growth potential.
Results
When C. reinhardtii and S. cerevisiae were co-cultured with an initial green algae to yeast inoculum ratio of 1:3, the cell concentration of C. reinhardtii reached 133 × 105 cells/mL on day 18 of culture, which was 1.5 times higher than that of the monoculture. Transcriptome analysis revealed that the expression levels of 363 green algae and 815 yeast genes were altered through co-cultivation. These included genes responsible for ammonium transport and CO2 enrichment mechanism in green algae and the genes responsible for glycolysis and stress responses in yeast.
Conclusion
We successfully increased C. reinhardtii growth potential by co-culturing it with S. cerevisiae. The main reasons for this are likely to be an increase in inorganic nitrogen available to green algae via yeast metabolism and an increase in energy available for green algae growth instead of CO2 enrichment.
期刊介绍：
Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them.
All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included.
Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields.
The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories.
Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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