完全体外迭代构建 24 kb 长的人工 DNA 序列，用于存储数字信息。

IF 2.2 4区工程技术 Q3 BIOCHEMICAL RESEARCH METHODS

BioTechniques Pub Date : 2024-04-04 DOI:10.2144/btn-2023-0109

Julien Leblanc, Olivier Boulle, Emeline Roux, Jacques Nicolas, Dominique Lavenier, Yann Audic

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引用次数: 0

摘要

在没有 DNA 模板的情况下，从头开始生产具有预定序列的长双链 DNA 分子尤其具有挑战性。DNA 合成步骤仍然是许多应用的瓶颈，如祖先基因的功能评估、替代剪接分析或基于 DNA 的数据存储。在本报告中，我们提出了一种完全体外的方案，利用黄金门组装技术，从市售的短 DNA 块开始，在不到 3 天的时间内生成超长的双链 DNA 分子。这一创新应用使我们能够简化流程，生成一个 24 kb 长的 DNA 分子，其中存储了 1789 年《人权和公民权利宣言》的部分内容。生成的 DNA 分子可以很容易地克隆到合适的宿主/载体系统中进行扩增和选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information.

In the absence of a DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.

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来源期刊

BioTechniques 工程技术-生化研究方法

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

3.3 months

期刊介绍： BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.