芯片上的植物:对番茄不同基因型的盐吸收和耐受性进行连续、无土电化学监测

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Philip Coatsworth, Yasin Cotur, Tarek Asfour, Zihao Zhou, José M. R. Flauzino, Tolga Bozkurt and Firat Güder
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引用次数: 0

摘要

番茄(Solanum lycopersicum)是一种高价值作物,它与盐有着独特的关系,NaCl 含量的增加可以提高风味、香气和营养质量,但也会造成氧化损伤并降低产量。考虑到农田中的钠含量随着时间的推移不断增加,人们对盐敏感性的重视程度有所降低。目前,还没有简单的方法来比较植物对盐的耐受性,在这种情况下,观察整个植物[Na+]的整体方法通常涉及破坏性的单个时间点测量或昂贵的成像技术。找到实时快速收集信息的方法可以提高对田间抗盐性的认识。在这里,我们使用 TETRIS(用于植物根部环境原位化学传感的时间分辨电化学技术)研究了番茄对 NaCl 的吸收情况,该平台用于测量活体植物根部区域的化学信号。我们使用低成本的丝网印刷电化学传感器,通过电阻抗测量来测量盐浓度的变化,从而便于监测根系对离子的吸收。我们不仅证明了不同生长条件下番茄幼苗对 NaCl 吸收速率的差异,而且还证明了对 NaCl 敏感性不同的番茄品种与相对耐盐的 "野番茄"(Solanum pimpinellifolium)姐妹品种之间的吸收差异。我们的研究结果表明,TETRIS 可用于确定成株但处于幼苗生长阶段的抗盐生理特性。这种推断方法以及多路复用和改变传感器配置的可能性,可以实现对成百上千个突变体或品种的高通量筛选。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Plant-on-a-chip: continuous, soilless electrochemical monitoring of salt uptake and tolerance among different genotypes of tomato†

Plant-on-a-chip: continuous, soilless electrochemical monitoring of salt uptake and tolerance among different genotypes of tomato†

Tomatoes (Solanum lycopersicum), a high-value crop, exhibit a unique relationship with salt, where increased levels of NaCl can enhance flavor, aroma and nutritional quality but can cause oxidative damage and reduce yields. A drive for larger, better-looking tomatoes has reduced the importance of salt sensitivity, a concern considering that the sodium content of agricultural land is increasing over time. Currently, there are no simple ways of comparing salt tolerance between plants, where a holistic approach looking at [Na+] throughout the plant typically involves destructive, single time point measurements or expensive imaging techniques. Finding methods that collect rapid information in real time could improve the understanding of salt resistance in the field. Here we investigate the uptake of NaCl by tomatoes using TETRIS (Time-resolved Electrochemical Technology for plant Root environment In situ chemical Sensing), a platform used to measure chemical signals in the root area of living plants. Low-cost, screen-printed electrochemical sensors were used to measure changes in salt concentration via electrical impedance measurements, facilitating the monitoring of the uptake of ions by roots. We not only demonstrated differences in the rate of uptake of NaCl between tomato seedlings under different growth conditions, but also showed differences in uptake between varieties of tomato with different NaCl sensitivities and the relatively salt-resistant “wild tomato” (Solanum pimpinellifolium) sister species. Our results suggest that TETRIS could be used to ascertain physiological traits of salt resistance found in adult plants but at the seedling stage of growth. This extrapolation, and the possibility to multiplex and change sensor configuration, could enable high-throughput screening of many hundreds or thousands of mutants or varieties.

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