新aurum 真菌重组菌株将植物甾醇转化为睾酮的单级生物转化过程

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-02 DOI:10.1134/s0026261723603913

D. N. Tekucheva, M. V. Karpov, V. V. Fokina, T. A. Timakova, A. A. Shutov, M. V. Donova

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引用次数: 0

摘要

摘要构建了一种质粒，其中含有编码真菌 17β-羟类固醇脱氢酶和霉菌葡萄糖-6-磷酸脱氢酶的基因。前者可催化 17-氧代基团的还原，后者可促进必需辅酶 NAD(P)H 的再循环。在经过充分研究的新牛磺酸分枝杆菌（Mycolicibacterium neoaurum）菌株中，该酶的组成型表达使 C-17 羟基类固醇的产量显著增加。其中，以新牛磺酸霉菌 VKM Ac-1815D 和新牛磺酸霉菌 NRRL B-3805 ΔkstD 为基础构建的重组菌株主要积累睾酮，而以新牛磺酸霉菌 VKM Ac-1816D 为基础构建的菌株则同时积累 1(2)-dehydrotestosterone 和睾酮。

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Single-Stage Bioconversion of Phytosterol into Testosterone by Recombinant Strains of Mycolicibacterium neoaurum

Abstract

A plasmid containing the genes encoding a fungal 17β-hydroxysteroid dehydrogenase, which ca-talyzes the reduction of 17-oxo group, and a mycobacterial glucose-6-phosphate dehydrogenase, which promotes the recycling of the essential coenzyme NAD(P)H, was constructed. Its constitutive expression in well-studied Mycolicibacterium neoaurum strains made it possible to increase the yield of C-17 hydroxysteroids significantly. In particular, recombinant strains created on the basis of M. neoaurum VKM Ac-1815D and M. neoaurum NRRL B-3805 ΔkstD exhibited predominant accumulation of testosterone, while the strain based on M. neoaurum VKM Ac-1816D accumulated 1(2)-dehydrotestosterone and testosterone simultaneously.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.