Hoang Thanh Tung, Nguyen Thi Nhu Mai, Do Manh Cuong, Nguyen Ba Nam, Trinh Thi Huy Tra, Hoang Ngoc Han, Nguyen Tran Vu, Ha Thi My Ngan, Bui Van The Vinh, Duong Tan Nhut
{"title":"通过微培系统提高茉莉花(Apocynaceae,Asclepiadoideae)离体小芽的生根能力","authors":"Hoang Thanh Tung, Nguyen Thi Nhu Mai, Do Manh Cuong, Nguyen Ba Nam, Trinh Thi Huy Tra, Hoang Ngoc Han, Nguyen Tran Vu, Ha Thi My Ngan, Bui Van The Vinh, Duong Tan Nhut","doi":"10.1007/s11240-024-02753-1","DOIUrl":null,"url":null,"abstract":"<p>A microponic system (MP system), a propagation system combines micropropagation and hydroponic that, has been studied on some flower and fruit plants; however, the MP system has not yet been tested on herbal plants, especially <i>Jasminanthes tuyetanhiae</i> (Apocynaceae, Asclepiadoideae). This study used the MP system with nylon film (A4 cover nylon) to evaluate rooting, abnormal developmental phenomena, antioxidant enzyme activities, secondary metabolites of plantlets, and subsequent growth. Results showed that 2-cm shoots cultured on a micropropagation system obtained a rooting rate of about 50–60%, and plantlets recorded abnormal phenomena reaching 85% consisting of 22.33% vitrification, 19.33% leaf abscission, 43.33% callus at the root and only 15% plantlets without abnormalities. In contrast, the MP system pre-treated with 0.5 mg/L indole butyric acid (IBA) and supplemented with 4 mg/L silver nanoparticles (AgNPs) obtained 100% rooting and improved plantlet quality. Furthermore, the plantlets developed using the MP system recorded a 2-fold reduction in vitrification and leaf abscission compared to those using the micropropagation system. A reduction in callus formation and increased antioxidant activities were also noted. The acclimatization of plantlets derived from the MP system pre-treated with 0.5 g/L IBA and 4.0 g/L AgNPs was higher than the conventional treatments.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"3 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced rooting in in vitro derived shootlets of Jasminanthes tuyetanhiae T.B. Tran & Rodda (Apocynaceae, Asclepiadoideae) through microponic system\",\"authors\":\"Hoang Thanh Tung, Nguyen Thi Nhu Mai, Do Manh Cuong, Nguyen Ba Nam, Trinh Thi Huy Tra, Hoang Ngoc Han, Nguyen Tran Vu, Ha Thi My Ngan, Bui Van The Vinh, Duong Tan Nhut\",\"doi\":\"10.1007/s11240-024-02753-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>A microponic system (MP system), a propagation system combines micropropagation and hydroponic that, has been studied on some flower and fruit plants; however, the MP system has not yet been tested on herbal plants, especially <i>Jasminanthes tuyetanhiae</i> (Apocynaceae, Asclepiadoideae). This study used the MP system with nylon film (A4 cover nylon) to evaluate rooting, abnormal developmental phenomena, antioxidant enzyme activities, secondary metabolites of plantlets, and subsequent growth. Results showed that 2-cm shoots cultured on a micropropagation system obtained a rooting rate of about 50–60%, and plantlets recorded abnormal phenomena reaching 85% consisting of 22.33% vitrification, 19.33% leaf abscission, 43.33% callus at the root and only 15% plantlets without abnormalities. In contrast, the MP system pre-treated with 0.5 mg/L indole butyric acid (IBA) and supplemented with 4 mg/L silver nanoparticles (AgNPs) obtained 100% rooting and improved plantlet quality. Furthermore, the plantlets developed using the MP system recorded a 2-fold reduction in vitrification and leaf abscission compared to those using the micropropagation system. A reduction in callus formation and increased antioxidant activities were also noted. The acclimatization of plantlets derived from the MP system pre-treated with 0.5 g/L IBA and 4.0 g/L AgNPs was higher than the conventional treatments.</p>\",\"PeriodicalId\":20219,\"journal\":{\"name\":\"Plant Cell, Tissue and Organ Culture\",\"volume\":\"3 1\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-04-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Cell, Tissue and Organ Culture\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11240-024-02753-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell, Tissue and Organ Culture","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02753-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Enhanced rooting in in vitro derived shootlets of Jasminanthes tuyetanhiae T.B. Tran & Rodda (Apocynaceae, Asclepiadoideae) through microponic system
A microponic system (MP system), a propagation system combines micropropagation and hydroponic that, has been studied on some flower and fruit plants; however, the MP system has not yet been tested on herbal plants, especially Jasminanthes tuyetanhiae (Apocynaceae, Asclepiadoideae). This study used the MP system with nylon film (A4 cover nylon) to evaluate rooting, abnormal developmental phenomena, antioxidant enzyme activities, secondary metabolites of plantlets, and subsequent growth. Results showed that 2-cm shoots cultured on a micropropagation system obtained a rooting rate of about 50–60%, and plantlets recorded abnormal phenomena reaching 85% consisting of 22.33% vitrification, 19.33% leaf abscission, 43.33% callus at the root and only 15% plantlets without abnormalities. In contrast, the MP system pre-treated with 0.5 mg/L indole butyric acid (IBA) and supplemented with 4 mg/L silver nanoparticles (AgNPs) obtained 100% rooting and improved plantlet quality. Furthermore, the plantlets developed using the MP system recorded a 2-fold reduction in vitrification and leaf abscission compared to those using the micropropagation system. A reduction in callus formation and increased antioxidant activities were also noted. The acclimatization of plantlets derived from the MP system pre-treated with 0.5 g/L IBA and 4.0 g/L AgNPs was higher than the conventional treatments.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.