用于分析药物制剂的亲水相互作用液相色谱法

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Current Analytical Chemistry Pub Date : 2024-03-08 DOI:10.2174/0115734110290557240305045032

Aleksandra Radoičić, Sandra Šegan, Aleksandra Dramićanin, Dušanka Milojković-Opsenica

{"title":"用于分析药物制剂的亲水相互作用液相色谱法","authors":"Aleksandra Radoičić, Sandra Šegan, Aleksandra Dramićanin, Dušanka Milojković-Opsenica","doi":"10.2174/0115734110290557240305045032","DOIUrl":null,"url":null,"abstract":"For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"90 1","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations\",\"authors\":\"Aleksandra Radoičić, Sandra Šegan, Aleksandra Dramićanin, Dušanka Milojković-Opsenica\",\"doi\":\"10.2174/0115734110290557240305045032\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.\",\"PeriodicalId\":10742,\"journal\":{\"name\":\"Current Analytical Chemistry\",\"volume\":\"90 1\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.2174/0115734110290557240305045032\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.2174/0115734110290557240305045032","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

摘要

长期以来，反相液相色谱法（RPLC）是分析药物化合物的最主要技术，但其分离小极性分子的效率较低。为了解决这些分子保留不充分的问题，近几十年来，一种名为亲水作用液相色谱（HILIC）的液相色谱模式得到了广泛推广。它的基础是使用高亲水性固定相和有机改性剂含量高的水性流动相。本综述介绍了 HILIC 中使用的固定相和流动相的特点，并讨论了相应的分离机制。综述了近期发表的有关 HILIC 在生物和非生物样品中药物分析应用的论文。此外，还介绍了 HILIC 系统在化合物理化性质测定中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations

For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Current Analytical Chemistry 化学-分析化学

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Current Analytical Chemistry publishes full-length/mini reviews and original research articles on the most recent advances in analytical chemistry. All aspects of the field are represented, including analytical methodology, techniques, and instrumentation in both fundamental and applied research topics of interest to the broad readership of the journal. Current Analytical Chemistry strives to serve as an authoritative source of information in analytical chemistry and in related applications such as biochemical analysis, pharmaceutical research, quantitative biological imaging, novel sensors, and nanotechnology.