Gyu Hyun Kim, Yong Hyun Lee, Ah Young Yoo, Shaheen Amna, Jae Kweon Park
{"title":"评估魔芋葡甘聚糖分子量变化对抗氧化剂和酪氨酸酶活性的影响","authors":"Gyu Hyun Kim, Yong Hyun Lee, Ah Young Yoo, Shaheen Amna, Jae Kweon Park","doi":"10.1007/s13233-024-00263-9","DOIUrl":null,"url":null,"abstract":"<div><p>The purpose of this study was to characterize the biological activity of konjac glucomannan (KGM) according to its molecular weight change. KGM-degrading bacterial strain, N3 was isolated from soil and named <i>Bacillus aerophilus</i> following 16S rDNA sequencing and homology analysis. KGMase, a thermophilic konjac glucomannan-degrading enzyme, was partially purified by treating the culture supernatant of strain <i>B. aerophilus</i> N3 with 50% ammonium sulfate followed by rigorous heating at 75 °C for 30 min. The molecular weight (MW) of KGMase was determined to be approximately 41,000 by silver staining following sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The biochemical properties stipulated that KGMase is optimally active at 45 °C and pH 6.5 which shows the high temperature and pH stability of KGMase. Manno-oligosaccharides (MOs) derived from the hydrolysis of KGM by KGMase showed a concentration-dependent increase in tyrosinase activity. MOs are bioactive compounds derived from the hydrolysis of KGM by the thermophilic KGMase of <i>B. aerophilus</i> and the outcomes of our study demonstrated that MOs will be suitable for application in the cosmetics industry.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":688,"journal":{"name":"Macromolecular Research","volume":"32 5","pages":"401 - 413"},"PeriodicalIF":2.8000,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the effect of molecular weight change of konjac glucomannan on antioxidant and tyrosinase activities\",\"authors\":\"Gyu Hyun Kim, Yong Hyun Lee, Ah Young Yoo, Shaheen Amna, Jae Kweon Park\",\"doi\":\"10.1007/s13233-024-00263-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The purpose of this study was to characterize the biological activity of konjac glucomannan (KGM) according to its molecular weight change. KGM-degrading bacterial strain, N3 was isolated from soil and named <i>Bacillus aerophilus</i> following 16S rDNA sequencing and homology analysis. KGMase, a thermophilic konjac glucomannan-degrading enzyme, was partially purified by treating the culture supernatant of strain <i>B. aerophilus</i> N3 with 50% ammonium sulfate followed by rigorous heating at 75 °C for 30 min. The molecular weight (MW) of KGMase was determined to be approximately 41,000 by silver staining following sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The biochemical properties stipulated that KGMase is optimally active at 45 °C and pH 6.5 which shows the high temperature and pH stability of KGMase. Manno-oligosaccharides (MOs) derived from the hydrolysis of KGM by KGMase showed a concentration-dependent increase in tyrosinase activity. MOs are bioactive compounds derived from the hydrolysis of KGM by the thermophilic KGMase of <i>B. aerophilus</i> and the outcomes of our study demonstrated that MOs will be suitable for application in the cosmetics industry.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":688,\"journal\":{\"name\":\"Macromolecular Research\",\"volume\":\"32 5\",\"pages\":\"401 - 413\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Macromolecular Research\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13233-024-00263-9\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Macromolecular Research","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13233-024-00263-9","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
Evaluation of the effect of molecular weight change of konjac glucomannan on antioxidant and tyrosinase activities
The purpose of this study was to characterize the biological activity of konjac glucomannan (KGM) according to its molecular weight change. KGM-degrading bacterial strain, N3 was isolated from soil and named Bacillus aerophilus following 16S rDNA sequencing and homology analysis. KGMase, a thermophilic konjac glucomannan-degrading enzyme, was partially purified by treating the culture supernatant of strain B. aerophilus N3 with 50% ammonium sulfate followed by rigorous heating at 75 °C for 30 min. The molecular weight (MW) of KGMase was determined to be approximately 41,000 by silver staining following sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The biochemical properties stipulated that KGMase is optimally active at 45 °C and pH 6.5 which shows the high temperature and pH stability of KGMase. Manno-oligosaccharides (MOs) derived from the hydrolysis of KGM by KGMase showed a concentration-dependent increase in tyrosinase activity. MOs are bioactive compounds derived from the hydrolysis of KGM by the thermophilic KGMase of B. aerophilus and the outcomes of our study demonstrated that MOs will be suitable for application in the cosmetics industry.
期刊介绍：
Original research on all aspects of polymer science, engineering and technology, including nanotechnology
Presents original research articles on all aspects of polymer science, engineering and technology
Coverage extends to such topics as nanotechnology, biotechnology and information technology
The English-language journal of the Polymer Society of Korea
Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.
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