生物合成纳米陶瓷对 MCF-7 乳腺癌细胞系的体外细胞毒性评估

IF 7.5 Q1 CHEMISTRY, PHYSICAL
Hendry Moses Panneerselvam , Z. Mohamed Riyas , M. Ramesh Prabhu , Moorthy Sasikumar , Ebenezar Jeyasingh
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引用次数: 0

摘要

由于乳腺癌的发病率和死亡率不断上升,它已成为一个重大的健康问题。因此,由于传统治疗方法会产生严重的不良反应,而且疗效有限,因此需要迅速采取行动,开发创新的治疗干预措施。本研究旨在利用生物合成的纳米铈开发一种创新的乳腺癌治疗干预方法。纳米铈具有生物相容性、抗氧化和促氧化双重功能以及对癌细胞的特异性细胞毒性,因此在医学领域具有重要意义。本文利用越橘谷物提取物(EFNC)的生态友好性和成本效益,对纳米铈进行了生物合成,并采用标准表征技术对其进行了系统研究。此外,还使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)细胞活力测定法研究了它们对 MCF-7 乳腺癌细胞株的抗癌功效,并使用吖啶橙/溴化乙锭(AO/EtBr)染色法测定了细胞凋亡的发生情况。值得注意的是,即使浓度较低(10 微克/毫升),EFNC 也表现出卓越的抗癌功效,其 IC50 值为 47.32 微克/毫升。此外,在AO/EtBr染色中观察到的亮绿色和橙红色荧光以及染色质碎裂/凝结特征清楚地表明,EFNC通过细胞凋亡途径导致细胞死亡。导致 EFNC 抗癌效果的一个潜在因素可能是它在癌细胞中的促氧化特性。这些特性最终会增强氧化应激,诱导活性氧(ROS)的积累,并导致细胞凋亡。因此,体外分析证实了 EFNC 的显著抗癌功效,表明它有可能成为抗癌治疗中一种有前途的治疗剂。这些发现为进一步探索和开发 EFNC 铺平了道路,使其成为应对癌症相关挑战的宝贵候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro cytotoxicity assessment of biosynthesized nanoceria against MCF-7 breast cancer cell lines

Breast cancer poses a significant health concern due to its increasing prevalence and mortality rates. Hence, prompt action is needed to develop innovative therapeutic interventions, as conventional treatments exhibit severe adverse effects and have limited efficacy. This study aims to develop an innovative therapeutic intervention for breast cancer using biosynthesized nanoceria. Nanoceria has gained significant importance in the medicinal field due to its biocompatibility, dual capabilities as both antioxidant and prooxidant, and specific cytotoxicity towards cancer cells. Herein, nanoceria was biosynthesized using Echinochloa frumentaceae grain extract (EFNC) due to its eco-friendly and cost-efficient nature, and it was systematically investigated using standard characterization techniques. Furthermore, their anticancer efficacy against MCF-7 breast cancer cell lines was studied using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell viability assay, and the occurrence of apoptosis was determined using acridine orange/ethidium bromide (AO/EtBr) staining. Significantly, EFNC exhibited superior anticancer efficacy even at lower concentrations (10 µg/mL), and the IC50 value was found at 47.32 µg/mL. In addition, the observed bright green and orange-red fluorescence, along with fragmented/condensed chromatin features in AO/EtBr staining clearly indicated that the EFNC caused cell death through the apoptotic route. One potential factor contributing to the observed anticancer effectiveness of EFNC could be its prooxidant properties within cancer cells. These properties ultimately enhanced oxidative stress, induced the accumulation of reactive oxygen species (ROS), and led to apoptosis. Hence, the in vitro analysis substantiated the significant anticancer efficacy of EFNC, suggesting its potential utility as a promising therapeutic agent in anticancer treatment. These findings pave the way for further exploration and development of EFNC as a valuable candidate for addressing cancer-related challenges.

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CiteScore
8.10
自引率
1.60%
发文量
128
审稿时长
66 days
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