A.M. Shurrab , A.T. Shatarat , M.H. Al-Muhtaseb , D.H. Badran , M.A. Salameh , H.M. Al-lahham , R.K. Altaweel , I. Altarawneh , D. Al-Qattan
{"title":"鸢尾素对大鼠胸主动脉超微结构的影响：形态计量学研究","authors":"A.M. Shurrab , A.T. Shatarat , M.H. Al-Muhtaseb , D.H. Badran , M.A. Salameh , H.M. Al-lahham , R.K. Altaweel , I. Altarawneh , D. Al-Qattan","doi":"10.1016/j.morpho.2024.100779","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>One of the most recent hormones to be identified and isolated is irisin, extracted from mouse skeletal muscle in 2012. Irisin has been proven to alter blood pressure, which has an impact on blood vessels, enhance endothelial functions, and prevent injury to endothelial cells. The current study aimed to study the effect of irisin on the ultrastructure of the rat thoracic aorta using the transmission electron microscope (TEM).</p></div><div><h3>Materials and methods</h3><p>Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250<!--> <!-->ng/mL, 500<!--> <!-->ng/mL, 1000<!--> <!-->ng/mL, and 2000<!--> <!-->ng/mL) twice a week for 4<!--> <!-->weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.</p></div><div><h3>Results</h3><p>The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (<em>P</em> <!--><<!--> <!-->0.0001) and smooth muscle cells (<em>P</em> <!--><<!--> <!-->0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (<em>P</em> <!--><<!--> <!-->0.0001).</p></div><div><h3>Conclusion</h3><p>Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of irisin on the ultrastructure of the thoracic aorta in rat: A morphometric study\",\"authors\":\"A.M. Shurrab , A.T. Shatarat , M.H. Al-Muhtaseb , D.H. Badran , M.A. Salameh , H.M. Al-lahham , R.K. Altaweel , I. Altarawneh , D. Al-Qattan\",\"doi\":\"10.1016/j.morpho.2024.100779\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>One of the most recent hormones to be identified and isolated is irisin, extracted from mouse skeletal muscle in 2012. Irisin has been proven to alter blood pressure, which has an impact on blood vessels, enhance endothelial functions, and prevent injury to endothelial cells. The current study aimed to study the effect of irisin on the ultrastructure of the rat thoracic aorta using the transmission electron microscope (TEM).</p></div><div><h3>Materials and methods</h3><p>Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250<!--> <!-->ng/mL, 500<!--> <!-->ng/mL, 1000<!--> <!-->ng/mL, and 2000<!--> <!-->ng/mL) twice a week for 4<!--> <!-->weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.</p></div><div><h3>Results</h3><p>The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (<em>P</em> <!--><<!--> <!-->0.0001) and smooth muscle cells (<em>P</em> <!--><<!--> <!-->0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (<em>P</em> <!--><<!--> <!-->0.0001).</p></div><div><h3>Conclusion</h3><p>Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.</p></div>\",\"PeriodicalId\":39316,\"journal\":{\"name\":\"Morphologie\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Morphologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1286011524000183\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Morphologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1286011524000183","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
The effect of irisin on the ultrastructure of the thoracic aorta in rat: A morphometric study
Background
One of the most recent hormones to be identified and isolated is irisin, extracted from mouse skeletal muscle in 2012. Irisin has been proven to alter blood pressure, which has an impact on blood vessels, enhance endothelial functions, and prevent injury to endothelial cells. The current study aimed to study the effect of irisin on the ultrastructure of the rat thoracic aorta using the transmission electron microscope (TEM).
Materials and methods
Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250 ng/mL, 500 ng/mL, 1000 ng/mL, and 2000 ng/mL) twice a week for 4 weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.
Results
The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (P < 0.0001) and smooth muscle cells (P < 0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (P < 0.0001).
Conclusion
Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.
期刊介绍：
Morphologie est une revue universitaire avec une ouverture médicale qui sa adresse aux enseignants, aux étudiants, aux chercheurs et aux cliniciens en anatomie et en morphologie. Vous y trouverez les développements les plus actuels de votre spécialité, en France comme a international. Le objectif de Morphologie est d?offrir des lectures privilégiées sous forme de revues générales, d?articles originaux, de mises au point didactiques et de revues de la littérature, qui permettront notamment aux enseignants de optimiser leurs cours et aux spécialistes d?enrichir leurs connaissances.
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