用于微秒时间分辨冷冻电镜的定形激光脉冲:闪熔过程中的超速结晶现象

IF 4.6 2区 化学 Q2 CHEMISTRY, PHYSICAL
Constantin R. Krüger, Nathan J. Mowry, Marcel Drabbels and Ulrich J. Lorenz*, 
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引用次数: 0

摘要

如果冷却速度超过 3 × 105 K/s,水就会玻璃化。与此相反,正如我们最近所展示的那样,当产生的无定形冰被瞬间加热时,即使加热速率高出 10 倍以上也会发生结晶。这可能会给用激光脉冲短暂熔化玻璃体冰样品的微秒时间分辨冷冻电镜实验带来问题,因为瞬时结晶可能会改变嵌入蛋白质的动态。在这里,我们展示了如何利用定形微秒激光脉冲来提高加热速率并超越结晶。时间分辨电子衍射实验显示,无定形固体水(ASW)的临界加热速率约为 108 K/s。我们的实验为新兴的微秒时间分辨低温电子显微镜领域增添了新的工具箱,展示了一种在激光熔化过程中避免结晶的直接方法,以及实现显著提高加热速率的方法,这为纳秒时间分辨实验铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Shaped Laser Pulses for Microsecond Time-Resolved Cryo-EM: Outrunning Crystallization during Flash Melting

Shaped Laser Pulses for Microsecond Time-Resolved Cryo-EM: Outrunning Crystallization during Flash Melting

Shaped Laser Pulses for Microsecond Time-Resolved Cryo-EM: Outrunning Crystallization during Flash Melting

Water vitrifies if cooled at rates above 3 × 105 K/s. In contrast, when the resulting amorphous ice is flash heated, crystallization occurs even at a more than 10 times higher heating rate, as we have recently shown. This may present an issue for microsecond time-resolved cryo-electron microscopy experiments, in which vitreous ice samples are briefly melted with a laser pulse because transient crystallization could potentially alter the dynamics of the embedded proteins. Here, we demonstrate how shaped microsecond laser pulses can be used to increase the heating rate and outrun crystallization. Time-resolved electron diffraction experiments reveal that the critical heating rate for amorphous solid water (ASW) is about 108 K/s. Our experiments add to the toolbox of the emerging field of microsecond time-resolved cryo-electron microscopy by demonstrating a straightforward approach for avoiding crystallization during laser melting and for achieving significantly higher heating rates, which paves the way for nanosecond time-resolved experiments.

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来源期刊
The Journal of Physical Chemistry Letters
The Journal of Physical Chemistry Letters CHEMISTRY, PHYSICAL-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
9.60
自引率
7.00%
发文量
1519
审稿时长
1.6 months
期刊介绍: The Journal of Physical Chemistry (JPC) Letters is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, chemical physicists, physicists, material scientists, and engineers. An important criterion for acceptance is that the paper reports a significant scientific advance and/or physical insight such that rapid publication is essential. Two issues of JPC Letters are published each month.
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