Yangyang Wang, Furui Yang, Yingyi Fu, Xin He, Haowei Tian, Lili Yang, Mengxi Wu, Jijuan Cao and Junshan Liu
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引用次数: 0
摘要
如今,转基因食品仍然备受争议。由于世界各国的政策和态度不尽相同,进口管理、市场监管等部门对快速、经济、方便的转基因作物鉴定方法的需求日益增长。然而,核酸鉴定方法作为目前最被认可的方法,需要笨重的仪器、较长的周转时间和训练有素的人员,仅适用于实验室。为了满足现场检测的迫切需求,我们开发了一种床旁检测平台,能够在 40 分钟内识别 12 种转基因作物,而无需使用实验室环境。我们的系统在微流控芯片中集成了样品预处理模块,通过电池供电的便携式试剂盒进行 DNA 扩增,并通过眼睛识别的比色变化显示结果。我们开发了一种基于石蜡的回流方法和一种基于滑板的流体开关,可按需在单个微孔中封装和释放扩增引物,从而同时鉴定不同的目标。我们的系统为转基因作物鉴定提供了一种高效、经济、便捷的工具,因此它不仅有利于海关和市场管理局,还能满足众多消费者的需求。
A point-of-care testing platform for on-site identification of genetically modified crops†
Genetically modified (GM) food is still highly controversial nowadays. Due to the disparate policies and attitudes worldwide, demands for a rapid, cost-effective and user-friendly GM crop identification method are increasingly significant for import administration, market supervision, etc. However, as the most-recognized methods, nucleic acid-based identification approaches require bulky instruments, long turn-around times and trained personnel, which are only suitable in laboratories. To fulfil the urgent needs of on-site testing, we develop a point-of-care testing platform that is able to identify 12 types of GM crops in less than 40 minutes without using laboratory settings. Our system integrates sample pre-treatment modules in a microfluidic chip, performs DNA amplification via a battery-powered portable kit, and presents results via eye-recognized colorimetric change. A paraffin-based reflow method and a slip plate-based fluid switch are developed to encapsulate and release amplification primers in individual microwells on demand, thus enabling identification of varied targets simultaneously. Our system offers an efficient, affordable and convenient tool for GM crop identification, thus it will not only benefit customs and market administration bureaus, but also satisfy demands of numerous consumers.
期刊介绍:
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.