{"title":"使用 GC-MS 和基于 HPTLC 的生物分析方法，对印度曼尼普尔省生长的 Osbeckia stellata var.","authors":"Tania Baishya , Priya Das , Gouhar Jahan Ashraf , Tarun Kumar Dua , Paramita Paul , Gouranga Nandi , Honey Jajo , Ankita Dutta , Anoop Kumar , Malay Bhattacharya , Ranabir Sahu","doi":"10.1016/j.kjs.2024.100229","DOIUrl":null,"url":null,"abstract":"<div><p>In the present study, we aimed to investigate the antioxidant, cytotoxicity activities, and phytochemical profiling of <em>O. stellata</em> extracts. Qualitative phytochemical tests showed the presence of different groups of phytochemicals, mainly in polar solvents. Methanolic and hydroalcoholic extracts showed high total phenolic and flavonoid contents. The antioxidant activity was determined by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), metal chelating, and reducing power assay where methanolic (IC<sub>50</sub>: 24.76 ± 2.78 μg/mL), hydroalcoholic (IC<sub>50</sub>: 29.59 ± 3.44 μg/mL), and aqueous (IC<sub>50</sub>: 33.72 ± 1.97 μg/mL) extracts showed significantly antioxidant potential. Significant cytotoxicity activity was observed in the methanolic extract. As methanolic extract showed better activities, gas chromatography–mass spectrometry (GC-MS) analysis of methanolic extract identified 41 bioactive compounds, where 17 major compounds had medicinal value. High-performance thin layer chromatography (HPTLC) bioassay-guided assay combined with Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and nuclear magnetic resonance (NMR) spectroscopy characterized gallic and caffeic acid as free radical scavengers. This study evidences that the methanolic extract of <em>O. stellata</em> has potent antioxidant and anti-cancer properties, which may render oxidative stress-related disease and may be used for drug development for human health benefits.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"51 3","pages":"Article 100229"},"PeriodicalIF":1.2000,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824000543/pdfft?md5=f676b0ad453285f62bde61f642f55014&pid=1-s2.0-S2307410824000543-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Antioxidant activity, cytotoxicity assay, and evaluation of bioactive compounds using GC-MS and HPTLC-based bioassay in the extracts of Osbeckia stellata var. crinita (Benth. ex Naudin) grown in Manipur, India\",\"authors\":\"Tania Baishya , Priya Das , Gouhar Jahan Ashraf , Tarun Kumar Dua , Paramita Paul , Gouranga Nandi , Honey Jajo , Ankita Dutta , Anoop Kumar , Malay Bhattacharya , Ranabir Sahu\",\"doi\":\"10.1016/j.kjs.2024.100229\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In the present study, we aimed to investigate the antioxidant, cytotoxicity activities, and phytochemical profiling of <em>O. stellata</em> extracts. Qualitative phytochemical tests showed the presence of different groups of phytochemicals, mainly in polar solvents. Methanolic and hydroalcoholic extracts showed high total phenolic and flavonoid contents. The antioxidant activity was determined by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), metal chelating, and reducing power assay where methanolic (IC<sub>50</sub>: 24.76 ± 2.78 μg/mL), hydroalcoholic (IC<sub>50</sub>: 29.59 ± 3.44 μg/mL), and aqueous (IC<sub>50</sub>: 33.72 ± 1.97 μg/mL) extracts showed significantly antioxidant potential. Significant cytotoxicity activity was observed in the methanolic extract. As methanolic extract showed better activities, gas chromatography–mass spectrometry (GC-MS) analysis of methanolic extract identified 41 bioactive compounds, where 17 major compounds had medicinal value. High-performance thin layer chromatography (HPTLC) bioassay-guided assay combined with Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and nuclear magnetic resonance (NMR) spectroscopy characterized gallic and caffeic acid as free radical scavengers. This study evidences that the methanolic extract of <em>O. stellata</em> has potent antioxidant and anti-cancer properties, which may render oxidative stress-related disease and may be used for drug development for human health benefits.</p></div>\",\"PeriodicalId\":17848,\"journal\":{\"name\":\"Kuwait Journal of Science\",\"volume\":\"51 3\",\"pages\":\"Article 100229\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2307410824000543/pdfft?md5=f676b0ad453285f62bde61f642f55014&pid=1-s2.0-S2307410824000543-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kuwait Journal of Science\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2307410824000543\",\"RegionNum\":4,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kuwait Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2307410824000543","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Antioxidant activity, cytotoxicity assay, and evaluation of bioactive compounds using GC-MS and HPTLC-based bioassay in the extracts of Osbeckia stellata var. crinita (Benth. ex Naudin) grown in Manipur, India
In the present study, we aimed to investigate the antioxidant, cytotoxicity activities, and phytochemical profiling of O. stellata extracts. Qualitative phytochemical tests showed the presence of different groups of phytochemicals, mainly in polar solvents. Methanolic and hydroalcoholic extracts showed high total phenolic and flavonoid contents. The antioxidant activity was determined by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), metal chelating, and reducing power assay where methanolic (IC50: 24.76 ± 2.78 μg/mL), hydroalcoholic (IC50: 29.59 ± 3.44 μg/mL), and aqueous (IC50: 33.72 ± 1.97 μg/mL) extracts showed significantly antioxidant potential. Significant cytotoxicity activity was observed in the methanolic extract. As methanolic extract showed better activities, gas chromatography–mass spectrometry (GC-MS) analysis of methanolic extract identified 41 bioactive compounds, where 17 major compounds had medicinal value. High-performance thin layer chromatography (HPTLC) bioassay-guided assay combined with Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and nuclear magnetic resonance (NMR) spectroscopy characterized gallic and caffeic acid as free radical scavengers. This study evidences that the methanolic extract of O. stellata has potent antioxidant and anti-cancer properties, which may render oxidative stress-related disease and may be used for drug development for human health benefits.
期刊介绍：
Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.
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