氧化锌-氧化锰/羧甲基纤维素-叶酸-芝麻酚混合纳米材料：治疗晚期三阴性乳腺癌的分子靶向策略

IF 3.8 4区工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY

Green Processing and Synthesis Pub Date : 2024-01-01 DOI:10.1515/gps-2023-0179

Chunming Zhao, Xueqiang Pan, Xiao Li, Meixia Li, Rui Jiang, Yuyang Li

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引用次数: 0

摘要

多功能纳米复合材料（NC）可降低肿瘤的增殖潜力，从而大大提高治疗效果。在目前的工作中，我们创造了一类新型的 Zn_Mn_CMC_FA_sesamol NC，用于对抗乳腺癌（MDA-MB-231）细胞。为了了解锌（Zn）、锰（Mn）、羧甲基纤维素和叶酸（FA）如何与芝麻酚相互作用，我们使用紫外-可见分光光度计和傅立叶变换红外光谱分析了合成的 NC 的吸收行为。X 射线衍射和动态光散射证实了 NC 的粒度。扫描电子显微镜用于评估这些 NC 的形态特征。光致发光光谱用于分析样品的光学和电子转变分子。除了 MTT 分析外，还使用吖啶橙/溴化乙锭（AO/EtBr）分析活性氧（ROS）、4′,6-二脒基-2-苯基吲哚进行细胞核染色以及流式细胞仪来证实 Zn_Mn_CMC_FA_sesamol NC 对 MDA-MB-231 细胞的凋亡活性。结果表明，与对照细胞相比，处理后的细胞具有明显的细胞毒性，AO/EtBr 诱导细胞凋亡，ROS 生成增加。细胞周期分析表明，NCs 会引发细胞凋亡，并使细胞周期停滞在 G0/G1 期。总之，所创造的 NC 是成功进行癌症分子靶向化疗的多功能平台。

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Zinc oxide-manganese oxide/carboxymethyl cellulose-folic acid-sesamol hybrid nanomaterials: A molecularly targeted strategy for advanced triple-negative breast cancer therapy

Multifunctional nanocomposites (NC) can greatly enhance therapy outcomes by reducing tumor proliferative potential. We created a novel class of Zn_Mn_CMC_FA_sesamol NC in the current work to combat breast cancer (MDA-MB-231) cells. To understand how zinc (Zn), manganese (Mn), carboxymethylcellulose, and folic acid (FA) interact with sesamol, UV-Visible spectrophotometer and Fourier Transform Infrared spectroscopy were used to analyze the absorption behavior of the synthesized NC. The particle size of NC was confirmed by X-ray diffraction and dynamic light scattering. Scanning electron microscopy was used to assess the morphological features of these NCs. photoluminescence spectrum was used to analyze the optical and electron transition molecules of the sample. In addition to MTT analysis, acridine orange/ethidium bromide (AO/EtBr) analysis of reactive oxygen species (ROS) and nuclear staining with 4′,6-diamidino-2-phenylindole as well as flow cytometry were used to confirm the apoptotic activity of Zn_Mn_CMC_FA_sesamol NC on MDA-MB-231 cells. The results showed significant cytotoxicity, apoptosis induction on AO/EtBr, and increased ROS production in treated cells compared to control cells. The cell cycle analysis revealed that NCs triggered apoptosis and arrested the cell cycle in G0/G1 phases. As a conclusion, the created NC serves as a versatile platform for the successful molecularly targeted chemotherapeutic treatment of cancer.

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来源期刊

Green Processing and Synthesis CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL

CiteScore

6.70

自引率

9.30%

发文量

审稿时长

7 weeks

期刊介绍： Green Processing and Synthesis is a bimonthly, peer-reviewed journal that provides up-to-date research both on fundamental as well as applied aspects of innovative green process development and chemical synthesis, giving an appropriate share to industrial views. The contributions are cutting edge, high-impact, authoritative, and provide both pros and cons of potential technologies. Green Processing and Synthesis provides a platform for scientists and engineers, especially chemists and chemical engineers, but is also open for interdisciplinary research from other areas such as physics, materials science, or catalysis.