Wenjun Wei , Tao Guo , Wenguang Fan , Mengshan Ji , Yu Fu , Conglong Lian , Suiqing Chen , Wenjing Ma , Wenfang Ma , Shuying Feng
{"title":"代谢组和转录组的整合分析为了解莉莉牛的功能成分提供了新的视角","authors":"Wenjun Wei , Tao Guo , Wenguang Fan , Mengshan Ji , Yu Fu , Conglong Lian , Suiqing Chen , Wenjing Ma , Wenfang Ma , Shuying Feng","doi":"10.1016/j.chmed.2023.10.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><em>Lilium brownii</em> var. <em>viridulum</em> (LB) and <em>L. lancifolium</em> (LL) are the main sources of medicinal lily (<em>Lilii Bulbus</em>, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of <em>Lilii Bulbus</em>, we selected <em>L. davidii</em> var. <em>willmottiae</em> (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL).</p></div><div><h3>Methods</h3><p>Metabolome and transcriptome were carried out to investigate the differences of active components in LD <em>vs</em> LB and LD <em>vs</em> LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis.</p></div><div><h3>Results</h3><p>The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD <em>vs</em> LB group and 298 SDMs in LD <em>vs</em> LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB <em>vs</em> LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD <em>vs</em> LB group and 18 958 DEGs in LD <em>vs</em> LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, <em>CYP724</em>, <em>CYP710A</em>, and <em>CYP734A1</em> in LB and <em>CYP90B</em> in LL were all up-regulated.</p></div><div><h3>Conclusion</h3><p>This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of <em>Lilii Bulbus</em>, which can provide new insights for <em>Lilii Bulbus</em> used in the research and development of classic famous formula.</p></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"16 3","pages":"Pages 435-448"},"PeriodicalIF":4.7000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1674638424000078/pdfft?md5=36e4859dc837025c25ed32688316af0b&pid=1-s2.0-S1674638424000078-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus\",\"authors\":\"Wenjun Wei , Tao Guo , Wenguang Fan , Mengshan Ji , Yu Fu , Conglong Lian , Suiqing Chen , Wenjing Ma , Wenfang Ma , Shuying Feng\",\"doi\":\"10.1016/j.chmed.2023.10.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p><em>Lilium brownii</em> var. <em>viridulum</em> (LB) and <em>L. lancifolium</em> (LL) are the main sources of medicinal lily (<em>Lilii Bulbus</em>, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of <em>Lilii Bulbus</em>, we selected <em>L. davidii</em> var. <em>willmottiae</em> (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL).</p></div><div><h3>Methods</h3><p>Metabolome and transcriptome were carried out to investigate the differences of active components in LD <em>vs</em> LB and LD <em>vs</em> LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis.</p></div><div><h3>Results</h3><p>The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD <em>vs</em> LB group and 298 SDMs in LD <em>vs</em> LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB <em>vs</em> LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD <em>vs</em> LB group and 18 958 DEGs in LD <em>vs</em> LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, <em>CYP724</em>, <em>CYP710A</em>, and <em>CYP734A1</em> in LB and <em>CYP90B</em> in LL were all up-regulated.</p></div><div><h3>Conclusion</h3><p>This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of <em>Lilii Bulbus</em>, which can provide new insights for <em>Lilii Bulbus</em> used in the research and development of classic famous formula.</p></div>\",\"PeriodicalId\":9916,\"journal\":{\"name\":\"Chinese Herbal Medicines\",\"volume\":\"16 3\",\"pages\":\"Pages 435-448\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1674638424000078/pdfft?md5=36e4859dc837025c25ed32688316af0b&pid=1-s2.0-S1674638424000078-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Herbal Medicines\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1674638424000078\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638424000078","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus
Objective
Lilium brownii var. viridulum (LB) and L. lancifolium (LL) are the main sources of medicinal lily (Lilii Bulbus, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of Lilii Bulbus, we selected L. davidii var. willmottiae (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL).
Methods
Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis.
Results
The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD vs LB group and 298 SDMs in LD vs LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB vs LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD vs LB group and 18 958 DEGs in LD vs LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, CYP724, CYP710A, and CYP734A1 in LB and CYP90B in LL were all up-regulated.
Conclusion
This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of Lilii Bulbus, which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.
期刊介绍：
Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included.
CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.
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