{"title":"纯化用于细菌转化的 3×Myc PKG-Puro-Poly A 基因","authors":"Khairil Pahmi, M. Sidratullah","doi":"10.37311/jsscr.v5i3.24069","DOIUrl":null,"url":null,"abstract":"Dysregulation of the oncoprotein c-Myc (Myc) is involved in many types of cancer. Myc is a sequence-specific transcription factor that regulates the transcription of genes involved in the control of cell proliferation and apoptosis through mechanisms that are not well understood. The method of this research is experimental. The experiment result will be described. Some processes will be done by polymerase chain reaction (PCR) to amplify the gene and then it will be extracted to pure the targeted gene. This research has been succesful to purify 3×Myc PKG-Puro-Poly A gene. c-MYC (hereinafter MYC) is an oncoprotein consisting of 439 amino acids contains a well-characterized C-terminal DNA compound and an N-terminal transactivation domain (TAD). The C-terminal region »100 residues comprises a basic leucine zipper-helix-loop-helix (bHLH-LZ) segment that regulates heterodimerization between MYC and its partner bHLH-LZ MAX mediate in their binding to gene promoters.","PeriodicalId":257265,"journal":{"name":"Journal Syifa Sciences and Clinical Research","volume":"5 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Purification of 3×Myc PKG-Puro-Poly A Gene for Bacterial Transformation\",\"authors\":\"Khairil Pahmi, M. Sidratullah\",\"doi\":\"10.37311/jsscr.v5i3.24069\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Dysregulation of the oncoprotein c-Myc (Myc) is involved in many types of cancer. Myc is a sequence-specific transcription factor that regulates the transcription of genes involved in the control of cell proliferation and apoptosis through mechanisms that are not well understood. The method of this research is experimental. The experiment result will be described. Some processes will be done by polymerase chain reaction (PCR) to amplify the gene and then it will be extracted to pure the targeted gene. This research has been succesful to purify 3×Myc PKG-Puro-Poly A gene. c-MYC (hereinafter MYC) is an oncoprotein consisting of 439 amino acids contains a well-characterized C-terminal DNA compound and an N-terminal transactivation domain (TAD). The C-terminal region »100 residues comprises a basic leucine zipper-helix-loop-helix (bHLH-LZ) segment that regulates heterodimerization between MYC and its partner bHLH-LZ MAX mediate in their binding to gene promoters.\",\"PeriodicalId\":257265,\"journal\":{\"name\":\"Journal Syifa Sciences and Clinical Research\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal Syifa Sciences and Clinical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37311/jsscr.v5i3.24069\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal Syifa Sciences and Clinical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37311/jsscr.v5i3.24069","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
许多类型的癌症都与癌蛋白 c-Myc（Myc）的失调有关。Myc是一种序列特异性转录因子，通过尚不十分清楚的机制调节参与控制细胞增殖和凋亡的基因的转录。本研究的方法是实验法。将对实验结果进行描述。 一些过程将通过聚合酶链反应（PCR）来扩增基因，然后提取纯化目标基因。c-MYC （以下简称 MYC）是一种由 439 个氨基酸组成的肿瘤蛋白，含有一个特征明确的 C 端 DNA 复合物和一个 N 端转录激活域（TAD）。C 端区域 "100 个残基包括一个基本亮氨酸拉链-螺旋-环-螺旋（bHLH-LZ）区段，该区段调节 MYC 与其伙伴 bHLH-LZ MAX 之间的异源二聚体，介导它们与基因启动子的结合。
Purification of 3×Myc PKG-Puro-Poly A Gene for Bacterial Transformation
Dysregulation of the oncoprotein c-Myc (Myc) is involved in many types of cancer. Myc is a sequence-specific transcription factor that regulates the transcription of genes involved in the control of cell proliferation and apoptosis through mechanisms that are not well understood. The method of this research is experimental. The experiment result will be described. Some processes will be done by polymerase chain reaction (PCR) to amplify the gene and then it will be extracted to pure the targeted gene. This research has been succesful to purify 3×Myc PKG-Puro-Poly A gene. c-MYC (hereinafter MYC) is an oncoprotein consisting of 439 amino acids contains a well-characterized C-terminal DNA compound and an N-terminal transactivation domain (TAD). The C-terminal region »100 residues comprises a basic leucine zipper-helix-loop-helix (bHLH-LZ) segment that regulates heterodimerization between MYC and its partner bHLH-LZ MAX mediate in their binding to gene promoters.
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