Sadhu Rama, Mohana Rao, Sravani Yandava, T. Mohit, Kumar Moses, K. A. Rupesh, K. Satyasree, K. Mamatha, Anuradha Argi
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The objective was to identify if there are patterns in the quality and purity of the extracted DNA based on the postmortem interval. Additionally, the study aimed to investigate if the cause of death influenced DNA extractability.Observation and Results: DNA can be extracted from postmortem blood within a timeframe of up to 72 hours after death, given that the deceased body was preserved in cold storage within 12 hours after death. Both the salting out method and the phenol-chloroform method yielded bands of comparable quality, with the phenol-chloroform method showing a slightly higher DNA yield. The average absorbance ratio was 1.4 for the salting out method and 1.6 for the phenol chloroform method, as determined using a Nanodrop.Conclusion: This study concluded that DNA extraction from postmortem blood samples is feasible within 72hours after death. The integrity of the DNA remained intact during this time, but the quality and purity gradually decreased as the postmortem interval increased. The cause of death did not significantly affect DNA extractability.","PeriodicalId":516766,"journal":{"name":"Indian Journal of Forensic Medicine & Toxicology","volume":"8 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA Extraction from Postmortem Blood: A Pilot Study for Advancing Molecular Diagnostics in Forensic Medicine Casework\",\"authors\":\"Sadhu Rama, Mohana Rao, Sravani Yandava, T. Mohit, Kumar Moses, K. A. Rupesh, K. Satyasree, K. Mamatha, Anuradha Argi\",\"doi\":\"10.37506/1p784p86\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: DNA’s role in forensic practice is widely acknowledged for its unparalleled accuracy in identification. While developed countries have established molecular autopsy programs as early as two decades ago, India is yet to initiate such a program. The isolation of DNA serves as the crucial first step in the molecular autopsy protocol. The postmortem blood sample is one of the good sources for DNA extraction which wasn’t considered with rigor by the scientific community so far.Aims and Objectives: The aim of this study was to investigate the specific time period within which DNA canbe effectively extracted from postmortem blood samples. The objective was to identify if there are patterns in the quality and purity of the extracted DNA based on the postmortem interval. Additionally, the study aimed to investigate if the cause of death influenced DNA extractability.Observation and Results: DNA can be extracted from postmortem blood within a timeframe of up to 72 hours after death, given that the deceased body was preserved in cold storage within 12 hours after death. Both the salting out method and the phenol-chloroform method yielded bands of comparable quality, with the phenol-chloroform method showing a slightly higher DNA yield. The average absorbance ratio was 1.4 for the salting out method and 1.6 for the phenol chloroform method, as determined using a Nanodrop.Conclusion: This study concluded that DNA extraction from postmortem blood samples is feasible within 72hours after death. The integrity of the DNA remained intact during this time, but the quality and purity gradually decreased as the postmortem interval increased. 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引用次数: 0
摘要
背景:DNA 在法医实践中的作用因其无与伦比的鉴定准确性而得到广泛认可。发达国家早在二十年前就建立了分子尸检计划,但印度尚未启动此类计划。DNA 分离是分子解剖方案中至关重要的第一步。死后血液样本是提取 DNA 的良好来源之一,但迄今为止科学界尚未对其进行严格考虑:本研究旨在调查从死后血液样本中有效提取 DNA 的特定时间段。目的是确定根据死后时间间隔提取 DNA 的质量和纯度是否存在模式。此外,研究还旨在调查死因是否会影响 DNA 的提取:观察与结果:鉴于死者尸体在死后 12 小时内被冷藏保存,DNA 可在死后 72 小时内从死后血液中提取。盐析法和苯酚-氯仿法得到的条带质量相当,苯酚-氯仿法的 DNA 得率稍高。用 Nanodrop 测定,盐析法和苯酚-氯仿法的平均吸光度比分别为 1.4 和 1.6:本研究得出结论,从死后 72 小时内的血液样本中提取 DNA 是可行的。在这段时间内,DNA 的完整性保持完好,但随着死后间隔时间的延长,DNA 的质量和纯度逐渐下降。死因对 DNA 的可提取性没有明显影响。
DNA Extraction from Postmortem Blood: A Pilot Study for Advancing Molecular Diagnostics in Forensic Medicine Casework
Background: DNA’s role in forensic practice is widely acknowledged for its unparalleled accuracy in identification. While developed countries have established molecular autopsy programs as early as two decades ago, India is yet to initiate such a program. The isolation of DNA serves as the crucial first step in the molecular autopsy protocol. The postmortem blood sample is one of the good sources for DNA extraction which wasn’t considered with rigor by the scientific community so far.Aims and Objectives: The aim of this study was to investigate the specific time period within which DNA canbe effectively extracted from postmortem blood samples. The objective was to identify if there are patterns in the quality and purity of the extracted DNA based on the postmortem interval. Additionally, the study aimed to investigate if the cause of death influenced DNA extractability.Observation and Results: DNA can be extracted from postmortem blood within a timeframe of up to 72 hours after death, given that the deceased body was preserved in cold storage within 12 hours after death. Both the salting out method and the phenol-chloroform method yielded bands of comparable quality, with the phenol-chloroform method showing a slightly higher DNA yield. The average absorbance ratio was 1.4 for the salting out method and 1.6 for the phenol chloroform method, as determined using a Nanodrop.Conclusion: This study concluded that DNA extraction from postmortem blood samples is feasible within 72hours after death. The integrity of the DNA remained intact during this time, but the quality and purity gradually decreased as the postmortem interval increased. The cause of death did not significantly affect DNA extractability.