2023 年拉斯维加斯 "马格拉奇 "联邦科学与技术研究所(FSBSI)葡萄栽培领域科技 活动优先方向的实施情况

V. Likhovskoi, N. V. Aleynikova, Svetlana Valentinovna Levchenko
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引用次数: 0

摘要

介绍有助于提高国内葡萄和葡萄酒产品产量的研究,包括在有机农业系统中的研究。研究对象是本地品种、经典品种和育种品种的葡萄。研究是在集体使用安培学藏品 "Magarach "中心以及克里米亚工业种植园的基础上,按照公认的原始方法进行的。为数据库提供了 50 个品种的特征,获得了 4 个有价值的性状来源,对野生葡萄进行了基因分型,确定了 99 个等位基因变体。为原生胼胝体和悬浮培养细胞的发育选择了最佳物质浓度。确定了生物活性物质对心脏形胚胎和鱼雷形胚胎发育的影响。对获得的 6 个 Podarok Magaracha 品种转基因品系进行了活体改造,以评估其抗寒性和抗冻性。在离体植物的无性繁殖过程中,样品保持在活跃和缓慢生长的条件下。获得了 14 个葡萄品种的无菌培养物。回收程序结束后,对植物材料中潜伏的植物病原体进行了分子诊断。通过分子诊断方法,确定了农杆菌(Agrobacterium tumefaciens)生物菌种。在分子诊断过程中,在样本中检测到了 Rupestris 茎点裂病毒。在复杂的农业气候参数和用于隔离风土的指数背景下,葡萄质量指标的动态变化取决于葡萄品种和种植地区,对此有了新的认识。已经确定了葡萄品种-根茎组合。继续建立数据集,以训练检测有害生物和葡萄植株生长发育病理的神经网络。对本土品种和马格拉奇研究所培育品种的有机葡萄栽培生产技术进行了测试。使用含钙制剂储存鲜食葡萄的技术得到了优化。首次开发了跨平台集成信息技术,用于在专门的 "诱捕器 "中用光照固定和神经网络检测害虫数量的硬件和软件综合体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IMPLEMENTATION OF PRIORITY DIRECTIONS OF SCIENTIFIC AND TECHNICAL ACTIVITIES OF THE FSBSI INSTITUTE «MAGARACH» OF THE RAS IN THE FIELD OF VITICULTURE IN 2023
Research is presented that contributes to an increase in the production of domestic grape and wine products, including in the system of organic farming. The object of research is grapes of native, classical and breeding varieties. The research was carried out on the basis of the Center for collective use of the Ampelographic collection «Magarach», as well as in industrial plantations of the Crimea according to generally accepted and original methods. Characteristics of 50 varieties for the database, 4 sources of valuable traits were obtained, genotyping of wild grapes was performed, 99 allele variants were identified. Optimal concentrations of substances for the development of proembryogenic calluses and cells in suspension cultures were selected. The influence of biologically active substances on the development of heart shaped and torpedo shaped embryos has been established. The obtained 6 transgenic lines of the Podarok Magaracha variety were adapted to in vivo conditions to assess their cold resistance and frost resistance. In the vegetative collection of plants in vitro, samples are maintained under conditions of active and slow growth. An aseptic culture of 14 grape varieties was obtained. The molecular diagnosis of the latent form of phytopathogens of plant material after the recovery procedures was carried out. The biovar Agrobacterium tumefaciens was identified by the method of molecular diagnostics. During molecular diagnostics, the Rupestris stem pitting virus was detected in the samples. New knowledge has been gained about the dynamics of changes in the quality indicators of grapes depending on the variety and region of cultivation against the background of a complex of agro-climatic parameters and indices used to isolate terroirs. Varietal-rootstock combinations have been identified. The formation of datasets has continued in order to train neural networks for the detection of harmful organisms and pathologies of the development of grape plants. The technology of organic viticulture production for autochthonous varieties and varieties of the Magarach Institute breeding has been tested. The technology of storing table grapes based on the use of calcium-containing preparations has been optimized. For the first time, an information technology of cross-platform integration has been developed for the implemented hardware and software complex for photo-fixation and neural network detection of the number of pests in specialized «traps».
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