Insaf Mansour, C. Ozay, Fadime Kose, Soraya Rahmanı, Omar Kharoubı
{"title":"酸枣仁的体外伤口愈合活性及其对大鼠的抗炎作用","authors":"Insaf Mansour, C. Ozay, Fadime Kose, Soraya Rahmanı, Omar Kharoubı","doi":"10.7546/crabs.2024.01.14","DOIUrl":null,"url":null,"abstract":"Jujube (Ziziphus jujuba) has a long history of use in traditional medicine. It has many pharmacological properties, including neuroprotective, antioxidative, hepatoprotective, anti-cancer, anti-inflammatory, and immunomodulatory. Thus, this study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Ziziphus (EEZ) fruit and its wound-healing effect. The anti-inflammatory activity of EEZ was assessed in vivo by carrageenan-induced paw edema test. NIH-3T3 mouse embryonic fibroblast cells were used to evaluate the wound healing effect of EEZ. The effect of the extract on wound-healing was examined by in vitro scratch test. Also, the level of C-reactive protein (CRP) in the serum of rats was determined using the CRP latex test kit. The ethanol extract of jujube fruit induced wound closure in NIH-3T3 fibroblast cells for 18 h (88.5%) at 1 μ\\umu g/mL concentration. Also, EEZ significantly inhibited carrageenan-induced edema in rat paws. However, a statistically significant decrease in CRP levels was observed in rats pre-treated with EEZ. As a result, Z. jujuba fruit extract had anti-inflammatory and wound healing effects, which might be strongly related to its phytochemical composition.","PeriodicalId":104760,"journal":{"name":"Proceedings of the Bulgarian Academy of Sciences","volume":"30 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats\",\"authors\":\"Insaf Mansour, C. Ozay, Fadime Kose, Soraya Rahmanı, Omar Kharoubı\",\"doi\":\"10.7546/crabs.2024.01.14\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Jujube (Ziziphus jujuba) has a long history of use in traditional medicine. It has many pharmacological properties, including neuroprotective, antioxidative, hepatoprotective, anti-cancer, anti-inflammatory, and immunomodulatory. Thus, this study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Ziziphus (EEZ) fruit and its wound-healing effect. The anti-inflammatory activity of EEZ was assessed in vivo by carrageenan-induced paw edema test. NIH-3T3 mouse embryonic fibroblast cells were used to evaluate the wound healing effect of EEZ. The effect of the extract on wound-healing was examined by in vitro scratch test. Also, the level of C-reactive protein (CRP) in the serum of rats was determined using the CRP latex test kit. The ethanol extract of jujube fruit induced wound closure in NIH-3T3 fibroblast cells for 18 h (88.5%) at 1 μ\\\\umu g/mL concentration. Also, EEZ significantly inhibited carrageenan-induced edema in rat paws. However, a statistically significant decrease in CRP levels was observed in rats pre-treated with EEZ. As a result, Z. jujuba fruit extract had anti-inflammatory and wound healing effects, which might be strongly related to its phytochemical composition.\",\"PeriodicalId\":104760,\"journal\":{\"name\":\"Proceedings of the Bulgarian Academy of Sciences\",\"volume\":\"30 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Bulgarian Academy of Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7546/crabs.2024.01.14\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Bulgarian Academy of Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7546/crabs.2024.01.14","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats
Jujube (Ziziphus jujuba) has a long history of use in traditional medicine. It has many pharmacological properties, including neuroprotective, antioxidative, hepatoprotective, anti-cancer, anti-inflammatory, and immunomodulatory. Thus, this study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Ziziphus (EEZ) fruit and its wound-healing effect. The anti-inflammatory activity of EEZ was assessed in vivo by carrageenan-induced paw edema test. NIH-3T3 mouse embryonic fibroblast cells were used to evaluate the wound healing effect of EEZ. The effect of the extract on wound-healing was examined by in vitro scratch test. Also, the level of C-reactive protein (CRP) in the serum of rats was determined using the CRP latex test kit. The ethanol extract of jujube fruit induced wound closure in NIH-3T3 fibroblast cells for 18 h (88.5%) at 1 μ\umu g/mL concentration. Also, EEZ significantly inhibited carrageenan-induced edema in rat paws. However, a statistically significant decrease in CRP levels was observed in rats pre-treated with EEZ. As a result, Z. jujuba fruit extract had anti-inflammatory and wound healing effects, which might be strongly related to its phytochemical composition.