从受污染土壤中分离出的一种细菌产生有机酸和溶解无机磷酸盐的能力

Sinal Tuscano, N. Gajbhiye
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摘要

许多农业土壤都有大量的磷(P)储备,其中大部分是由于频繁施用磷肥而积累起来的。然而,大约 95% 到 99% 的土壤磷以不溶性磷酸盐的形式存在,因此植物无法吸收。目前的研究对从受污染土壤中获得的细菌菌株进行了鉴定,结果表明该菌株具有溶解不溶性无机磷酸盐的能力。在孟买受污染的土壤中分离出了一种高效的磷酸盐溶解细菌。该分离菌的磷酸盐溶解指数是用磷酸钙补充的皮科夫斯卡娅(PVK)培养基进行评估的。在不断搅拌下生长七天后,培养基的 pH 值从 7.0 单位降至 3.5 单位。根据菌落形态、显微镜分析和 MALDI-TOF 测序,该细菌被鉴定为肺炎克雷伯氏菌。磷酸盐溶解与细菌在以葡萄糖为碳源的培养基中生长导致的 pH 值下降有关。这些磷酸盐溶解细菌分泌的有机酸是它们能够溶解无机磷酸盐的原因。气相色谱-质谱分析显示,在培养上清液中存在氨基甲酸、十二酸、四癸酸和三氟乙酸。通过磷钼酸盐测定法测定了该细菌溶解的磷酸盐量,发现其含量为 667.0 微克/毫升,远高于对照细菌金黄色葡萄球菌的 131.0 微克/毫升。据我们所知,这是第一份从孟买受污染土壤中分离出磷酸盐溶解细菌的报告。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PRODUCTION OF ORGANIC ACID AND SOLUBILIZATION OF INORGANIC PHOSPHATE BY A BACTERIUM ISOLATED FROM CONTAMINATED SOIL
Many agricultural soils have significant phosphorus (P) reserves, much of which builds up because of frequent P fertilizer applications. However, roughly 95 to 99% of soil phosphorus is found as insoluble phosphates and is therefore unavailable for plant uptake. The current investigation characterized a bacterial strain that was obtained from contaminated soil and showed the ability to solubilize insoluble inorganic phosphates. An efficient phosphate-solubilizing bacterium was isolated in polluted soil in Mumbai. The phosphate solubilization index of this isolate was assessed using tribasic calcium phosphate-supplemented Pikovskaya’s (PVK) medium. After growing under constant agitation for seven days, the medium pH decreased from 7.0 to 3.5 units. Based on the colony morphology, microscopic analysis, and MALDI-TOF sequencing, the bacterial isolate was identified as Klebsiella pneumoniae. Phosphate solubilization was linked to a pH drop caused by bacterial growth in a medium with glucose as a carbon source. The secretion of organic acids by these phosphate-solubilizing bacteria is responsible for their ability to solubilize inorganic phosphate. GC-MS analysis revealed the presence of carbamic acid, dodecanoic acid, tetra decanoic acid, and trifluoroacetic acid in the culture supernatant. The amount of phosphate solubilized by the bacterium was determined by phosphomolybdate assay and was found to be 667.0 ug/ml which was much higher than the control bacterium S. aureus which was 131.0 ug/ml. To the best of our knowledge, this is the first report mentioning the isolation of phosphate solubilizing bacterium from polluted soil in Mumbai.
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