人类呼吸道病毒传染性的实验室研究:实验条件、检测和对大气环境的抵抗力

IF 6.2 3区 综合性期刊 Q1 Multidisciplinary
Yaohao Hu , Shuyi Peng , Bojiang Su , Tao Wang , Juying Lin , Wei Sun , Xiaodong Hu , Guohua Zhang , Xinming Wang , Ping'an Peng , Xinhui Bi
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引用次数: 0

摘要

实验室环境中传染性病毒的环境稳定性对人类呼吸道病毒的传播潜力至关重要。在过去几十年的研究中,不同的实验技术或条件导致了对大气环境中病毒传染性稳定性的不同理解和预测。本文主要针对流感病毒和冠状病毒(包括严重急性呼吸系统综合征冠状病毒 2 和中东呼吸系统综合征冠状病毒),综述了模拟大气环境对呼吸道病毒感染性影响的现有知识。首先,我们总结了实验条件对病毒稳定性的影响;这些条件涉及病毒气溶胶的产生方法、老化和收集期间的储存方法、病毒类型和毒株、悬浮基质、初始接种量和浓度以及干燥过程。其次,我们总结并讨论了病毒传染性的检测方法及其缺点。最后,我们将综述研究的结果进行整合,以全面了解大气环境条件对传染性病毒(尤其是气溶胶病毒)衰变的影响。总之,本综述强调了在预测病毒在空气传播过程中保持感染性的能力方面存在的知识空白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Laboratory studies on the infectivity of human respiratory viruses: Experimental conditions, detections, and resistance to the atmospheric environment

The environmental stability of infectious viruses in the laboratory setting is crucial to the transmission potential of human respiratory viruses. Different experimental techniques or conditions used in studies over the past decades have led to diverse understandings and predictions for the stability of viral infectivity in the atmospheric environment. In this paper, we review the current knowledge on the effect of simulated atmospheric conditions on the infectivity of respiratory viruses, mainly focusing on influenza viruses and coronaviruses, including severe acute respiratory syndrome coronavirus 2 and Middle East respiratory syndrome coronavirus. First, we summarize the impact of the experimental conditions on viral stability; these involve the methods of viral aerosol generation, storage during aging and collection, the virus types and strains, the suspension matrixes, the initial inoculum volumes and concentrations, and the drying process. Second, we summarize and discuss the detection methods of viral infectivity and their disadvantages. Finally, we integrate the results from the reviewed studies to obtain an overall understanding of the effects of atmospheric environmental conditions on the decay of infectious viruses, especially aerosolized viruses. Overall, this review highlights the knowledge gaps in predicting the ability of viruses to maintain infectivity during airborne transmission.

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来源期刊
Fundamental Research
Fundamental Research Multidisciplinary-Multidisciplinary
CiteScore
4.00
自引率
1.60%
发文量
294
审稿时长
79 days
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