Dong‐liang Wu, Shumin Pang, Viola Röhrs, Johanna Berg, A. S. Ali, Yikun Mei, Mathias Ziersch, Beatrice Tolksdorf, Jens Kurreck
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Man vs. machine: Automated bioink mixing device improves reliability and reproducibility of bioprinting results compared to human operators
The bioink mixing process is highly relevant to the bioink quality, which is the basis for reproducible extrusion-based three-dimensional (3D) bioprinting (EBB). Currently, most bioinks mixed by skilled human operators show variations in terms of cell homogeneity and biological properties as well as other properties. For preparation of many types of bioinks, striking the balance between homogeneity and cell viability remains a major challenge. This study investigates the relationship between bioink homogeneity and mixing parameters, particularly mixing speed and number of exchanges, utilizing a customized automated device. We found that up to a certain point, increasing the rate of mixing led to a better distribution of cells within the bioink, but beyond that point, there was a detrimental effect on cell viability. In contrast, the mixing number had less impact on the physiological properties of the cells in the bioink. Furthermore, a comparison between skilled human and machine bioink mixing revealed that the machine consistently provided better outcomes in terms of bioink homogeneity, cell distribution, and cell viability, highlighting the advantages and importance of standardizing the bioink mixing process. The methodology and approaches in this study can improve the reproducibility and reliability of EBB bioink and may thereby advance the field of 3D bioprinting in various applications.
期刊介绍:
The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.