碳源及其不同浓度对香蕉品种 Chinichampa (AAB) 通过芽尖离体生根的影响

S. K. Ozukum, C. S. Maiti, Pankaj Shah, P. Alila, A. Sarkar, L. Hemanta, Khamrang Mathukmi
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引用次数: 0

摘要

体外繁殖的香蕉植株无病虫害、均匀一致,而且可以快速繁殖,因此正逐渐成为首选的种植材料。本调查于 2019-2022 年期间在那加兰大学 Medziphema 校区农业科学学院园艺系组织培养实验室进行。对不同参数记录的数据,即返青天数、多芽萌发所需天数、每个外植体产生的多芽数量以及30天后多芽的长度,进行了完全随机设计(CRD)。本研究揭示了各种碳源的重要性及其在通过芽尖培养进行香蕉微繁殖中的作用。在添加了 30 克葡萄糖的生根诱导培养基(RIM)中,生根所需的天数最少。在添加了 40 克、30 克和 20 克食糖的根诱导培养基中,30 天后的生根率(%)、每芽生根数和最长根的长度(厘米)均有所提高。同时,添加 40 克葡萄糖的 RIM 在 30 天后观察到最大的平均芽长(厘米),添加 30 克蔗糖的 RIM 每株小苗的叶片数最多。这些结果表明了碳源在香蕉微繁殖中的重要性。它们提高了植株再生能力,促进了无病无病毒香蕉小苗的快速繁殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Response of carbon sources and their various concentrations on in vitro rooting of banana cv. Chinichampa (AAB) through shoot tip ​
In vitro propagated banana plants are steadily becoming the planting material of choice because of disease free, uniformity and the possibility of rapid multiplication. The present investigation was carried out at the tissue culture laboratory, Department of Horticulture, School of Agricultural Sciences, Nagaland University, Medziphema campus during the year 2019-2022. Data recorded for different parameters viz. days to greening, days required for multiple buds’ initiation, number of multiple buds produced per explant and length of multiple buds after 30 days were subjected to completely randomized design (CRD). The present study revealed the importance of various carbon sources and their role in micropropagation of banana through shoot tip culture. The minimum days required for root initiation were observed in root induction media (RIM) supplemented with 30g glucose. Rooting percentage (%), number of roots per shoot and length of longest roots (cm) after 30 days was seen in RIM supplemented with table sugar at 40g, 30g and 20g respectively. Meanwhile maximum average length of shoot (cm) after 30 days was observed in RIM supplemented with 40g glucose and highest number of leaves per plantlets was recorded in RIM supplemented with 30g sucrose. The results indicate the significance of carbon sources in micropropagation of banana. They improved plant regeneration and facilitated rapid multiplication of disease and virus free banana plantlets.
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