M.Phaseolina的分子特征及其利用农用化学品和T.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-25 DOI:10.36103/4p59fb72

K. A. Sido, W. A. Hassan

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引用次数: 0

摘要

研究了壳聚糖（CH）和水杨酸（SA）等农用化学品（25、50 和 100 ppm）以及 4 ×106 的向日葵炭腐病病原菌 T. harzianum（Th）生物控制剂诱导的系统获得抗性（SAR）。结果取决于对土壤中病害严重程度和小圆菌体密度的估计。因此，将种子浸泡在 75 ppm 的 CH 中 6 小时，病害严重程度降低了 48.25%（p=0.05），土壤中存活的小圆菌体减少了 70%。事实证明，施用 50 和 75 ppm 的 SA 能明显降低炭腐病的严重程度，两种浓度分别降低 39% 和 37%，且不随 Th 的变化而变化。结果还证实，施用 75 ppm 的 CH 能显著降低病害严重程度 40.63%，与施用相同浓度的 SA 相似。然而，最低剂量的 SA（25 ppm）与 CH（75 ppm）相比，微小硬菌密度减少了 80.28%，而 CH（75 ppm）则减少了 74.91%。为了对病原体进行分子鉴定，使用 ITS4 和 ITS5 通用引物进行聚合酶链式反应 (PCR)，以扩增六种相思豆分离物（即 OL901219、OL636051、OL901220、OL901204、OL636050 和 OL636053）的 DNA 序列，并通过 BLAST 模式与 NCBI GenBank 数据库中的 rDNA 序列进行比对，结果显示整个相似率达到 100%。

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MOLECULAR CHARACTERIZATION OF M. PHASEOLINA AND ITS MANAGEMENT USING AGROCHEMICALS AND T. HARZIANUM

Systemic Acquired Resistance (SAR) induced by agrochemicals of chitosan (CH) and salicylic acid (SA) at (25, 50 and 100 ppm) , in addition to a biocontrol agent of T. harzianum (Th) at 4 ×106 were examined against M. phaseolina the causal agent of charcoal rot of sunflower. The results depended on estimation of diseases severity and microsclerotia density in the soil. Thus, the seeds immersion in CH 75 ppm for 6 h., gave the highest and considerable reduction (p=0.05) in disease severity by 48.25% and reduced microsclerotia survived in the soil up to 70%. Application of SA at 50 and 75 ppm proved an obvious reduction of charcoal rot severity by up to 39% and 37% for both concentrations, respectively and not varied with Th. The results also confirmed that CH at 75 ppm revealed significant reduction 40.63% in disease severity and similarized with SA at same concentration. However, the lowest dose of SA at 25 ppm realized the highest reduction of micro sclerotia density by 80.28 % compared to 74.91% when used CH at 75 ppm. For molecular identification of a pathogen Polymerase Chain Reaction (PCR) using ITS4 and ITS5 universal primers were applied to amplify and sequence of DNA for six isolates of M. phaseolina viz., OL901219, OL636051, OL901220, OL901204, OL636050 and OL636053 compared for identity of rDNA sequence according to NCBI GenBank databases by BLAST mode and the results showed the entire similarity ratio reached to 100%

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.