评估血浆活化培养基对乳腺癌细胞株生长相关成分的影响

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-25 DOI:10.36103/e5wb1502

Rawnaq Z. Fadhil, Mouruj A. Al aubaydi, Kadhim A. Aadim Researcher Prof

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引用次数: 0

摘要

本研究旨在评估血浆活化培养基（PAM）对乳腺癌细胞系（MCF7和AMJ13）与正常脂肪衍生干细胞（ASCs）的一些参数的影响。首先，直接检测了所用培养基（RPMI-1640 和 MEM）在不同照射时间（10、15、20 和 25 分钟）后的一氧化氮（NO）产生情况，结果表明随着照射时间的延长，NO 显著增加，特别是在 RPMI 中照射 20 和 25 分钟后，而 MEM 仅在 25 分钟后才显示出显著结果。研究了代谢参数，包括葡萄糖摄取量和乳酸脱氢酶（LDH）活性。结果发现，LDH 活性的降低与培养时间成正比，培养 48 小时后，LDH 活性的降低比培养 24 小时更明显，而培养基中的酶活性受到的影响很小。葡萄糖结果显示了相关的结果，因为与未处理的同系细胞相比，随着辐照剂量的增加，所有三种处理过的细胞系培养基中的葡萄糖浓度都升高了。

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ESTIMATION THE EFFECTS OF PLASMA ACTIVATED MEDIUM ON SOME COMPONENTS THAT RELATED WITH GROWING BREAST CANCER CELL LINES

This study was aimed to assess the influence of plasma activated medium (PAM) on some parameters of breast cancer cell lines (MCF7 and AMJ13) in comparison to normal adipose-derived stem cells (ASCs). First, nitric oxide (NO) production within the used media (RPMI-1640 and MEM) was examined beyond different exposure time (10,15,20, and 25) minutes directly, and the finding suggested a significant increase in NO with increasing exposure time particularly after 20 and 25 min of irradiation in RPMI, while MEM showed significant results only after 25 min. The metabolic parameters were studied including glucose uptake and lactate dehydrogenase (LDH) activity. The results found a decrease in LDH activity proportional to incubation times, as over incubation to 48 hrs the activity reduced more significantly than the 24 hours with minimal influence on the enzyme activity in the media. Correlated results were demonstrated in glucose results as it’s concentration was elevated in the media with increasing irradiation doses for all three treated cell lines in compared to untreated homologues.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.