挖掘细菌素肽的抗菌潜力:针对食品腐败沙雷氏菌的减法蛋白质组学方法

Qudsia Yousafi, Dua Fatima
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引用次数: 0

摘要

食品变质是一个严重的环境问题,也是食品工业关注的一个主要问题。细菌素作为一种天然食品防腐剂,有助于解决这些问题。111 种细菌素的肽序列来自 UniProt。对肽的理化性质和免疫原性进行了鉴定。最后筛选出 55 种无免疫原性的抗菌肽。从 UniProt 下载了 Serratia marcescens 的全蛋白质组,其中包含 4 706 个蛋白质。采用减法蛋白质组学方法筛选出目标致病蛋白。只有 72 个来自外膜、细胞质膜、细胞外和细胞质周围的蛋白质被选中作进一步分析。只有两种 S. marcescens 蛋白,即 UPI0002AF278A 和 UPI0003C9BA85 与 55 种细菌素对接。利用 Amber 工具对五种蛋白质-细菌素复合物进行了 MD 模拟。在 50ns 的 MD 模拟过程中,发现 UPI0003C9BA85-P36504、UPI0003C9BA85-P80925m 和 UPI0003C9BA85-P86394m 这三个复合物是稳定的。建议对这三种细菌素进行进一步的实验室测试,以便将其用作食品防腐剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unlocking the antibacterial potential of bacteriocin peptides: A subtractive proteomics approach to target food spoiling Serratia marcescens
Food deterioration is a serious environmental issue as well as a major concern of the food industry. Bacteriocins, a natural food preservative, can help resolve these issues. The peptide sequences of 111 bacteriocins were obtained from UniProt. The physicochemical properties and immunogenicity of peptides were identified. Final 55 non immunogenic antimicrobial peptides were selected. Full proteome of Serratia marcescens, containing 4,706 proteins, was downloaded from UniProt. The subtractive proteomic approaches were used to select target pathogenic proteins. Only seventy-two proteins from outer membrane, cytoplasmic membrane, extracellular and periplasm were selected for further analysis. Only two S. marcescens proteins, UPI0002AF278A and UPI0003C9BA85, were docked with 55 bacteriocins. Five protein-bacteriocin complexes were subjected to MD simulation by using Amber tool. Three complexes, UPI0003C9BA85-P36504, UPI0003C9BA85-P80925m and UPI0003C9BA85-P86394m were found stable during 50ns run of MD simulation. These three bacteriocins are suggested for further laboratory testing to be used as food preservatives
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