细菌--soryz 细菌性疾病病原体的拮抗剂

IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY
M. V. Reshetnikov, V. Patyka
{"title":"细菌--soryz 细菌性疾病病原体的拮抗剂","authors":"M. V. Reshetnikov, V. Patyka","doi":"10.15407/agrisp10.03.046","DOIUrl":null,"url":null,"abstract":"Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents of bacterial diseases of\nsoryz (Sorghum oryzoidum) and sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming\nbacteria were isolated from the soryz samples, collected in the fields of the experimental farm of the Uman National\nHorticulture University (Cherkasy region, Uman). Lactic acid bacteria were isolated from soryz plants, collected in\nthe private land plot, located between the villages of Teolyn, Vladyslavchyk, Kniazhyky in Monastyryshche com-\nmunity, Uman district, where Pershotravneve hamlet used to be situated. A total of 1,250 samples were analyzed.\nThe experiment had three repeats. Spore-forming and lactic acid bacteria were isolated from the surface of soryz\nplants on the firm ripe stage in summer while isolating phytopathogenic bacteria. The isolates of lactic acid bacteria-\nantagonists were also isolated from the inner part of winter stubble stalk of soryz, collected from the tilled field. The\nantagonistic activity of the strains of lactic acid bacteria and spore-forming bacteria, isolated from different ecological\nniches, to phytopathogens of soryz and sorghum crops was determined in vitro. The strains of Pseudomonas syringae,\nthe agents of soryz bacterial spots, were used as test-cultures: 211141a, 211141, 210341, 21034, and 210521, along\nwith the collection strains of phytopathogens: Pseudomonas syringae 8299, Pseudomonas syringae subsp. syringae\nUKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. The antagonistic activity of\nthe extracted isolates of spore-forming and lactic acid bacteria was studied using the method of radial strokes (joint\ncultivation of the antagonist and the strains under investigation). The bacterial isolates were deemed inactive if the\ngrowth delay zone was 0–5 mm (–), from 5 to 10 mm (+) – low activity, 11–20 mm (++) – moderate activity, over 20\nmm (+++) – high activity regarding the test-cultures. To check the effect of the isolate-antagonist of phytopathogenic\nbacteria, artificial infecting was conducted in the field conditions. For this purpose, a diurnal culture of the antagonist\nwas introduced into the stalk of plants in the concentration of 1×108 colony-forming units, and 24 h later, a culture of\ntest-strain of the phytopathogen was administered above the previous puncture. The results were evaluated 7–14 days\nafter the artificial infection. The experiment had three repeats. The isolates of bacteria which demonstrated their an-\ntagonistic properties regarding the phytopathogenic bacteria were identified by their morphological properties, Gram\nstaining, catalase test, profile of carbohydrate fermentation and mass-spectrometry (MALDI-TOF – Matrix Assisted\nLaser Desorption/Ionization) using VITEK MS mass-spectrometer. Results. Thirty-eight spore-forming bacterial iso-\nlates were extracted from soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 demonstrated their antagonistic\nactivity towards the investigated phytopathogenic bacteria. Isolate 21040 showed high antagonistic activity to most\ntest-strains of P. syringae from soryz (the zone of negative culture – 23–30 mm) and lower activity regarding the\ncollection cultures. Isolates B4 and AVS3 demonstrated their selective activity regarding the investigated phytopatho-\ngens. Twenty isolates of lactic acid bacteria were extracted. Higher antagonistic activity was noted for the isolates of\nlactic acid bacteria 8/1 and F1 to the strains of P. syringae, isolated from soryz and collection cultures. The highest\nantagonistic activity of isolate 8/1 was noted regarding test-strains of P. syringae 210521 and X. oryzae 8375 (the\nzone of negative culture – 40–35 mm). In the field conditions, the treatment of sorghum plants with F1 affected the\npathological process that developed due to the impact of the phytopathogenic bacteria P. syringae, which led to the\nreduction in disease symptoms. The taxonomic position of the isolates of bacteria, which seem to be promising for\nthe control of disease agents, was determined. In terms of morphology of cells and colonies, the biochemical profile,\nand mass-spectrometry MALDI-TOF, the spore-forming isolates 21040 and B4 were identified as Bacillus subtilis,\nand ASV3 – as Bacillus vallismortis. The identified isolates of lactic acid bacteria were Lactobacillus pentosus F1\nand Lactobacillus sakei 8/1. Conclusions. In addition to phytopathogenic bacteria, from soryz plants we isolated the\nstrains of spore-forming bacteria Bacillus subtilis 21040, B4, Bacillus vallismortis AVS3 and such lactic acid bacteria\nas Lactiplantibacillus pentosus and Lactobacillus sakei 8/1 (Latilactobacillus sakei 8/1), promising for the elaboration\nof methods for the biocontrol of the agents of bacterial diseases.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacteria-antagonists of the agents of soryz bacterial diseases\",\"authors\":\"M. V. Reshetnikov, V. Patyka\",\"doi\":\"10.15407/agrisp10.03.046\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents of bacterial diseases of\\nsoryz (Sorghum oryzoidum) and sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming\\nbacteria were isolated from the soryz samples, collected in the fields of the experimental farm of the Uman National\\nHorticulture University (Cherkasy region, Uman). Lactic acid bacteria were isolated from soryz plants, collected in\\nthe private land plot, located between the villages of Teolyn, Vladyslavchyk, Kniazhyky in Monastyryshche com-\\nmunity, Uman district, where Pershotravneve hamlet used to be situated. A total of 1,250 samples were analyzed.\\nThe experiment had three repeats. Spore-forming and lactic acid bacteria were isolated from the surface of soryz\\nplants on the firm ripe stage in summer while isolating phytopathogenic bacteria. The isolates of lactic acid bacteria-\\nantagonists were also isolated from the inner part of winter stubble stalk of soryz, collected from the tilled field. The\\nantagonistic activity of the strains of lactic acid bacteria and spore-forming bacteria, isolated from different ecological\\nniches, to phytopathogens of soryz and sorghum crops was determined in vitro. The strains of Pseudomonas syringae,\\nthe agents of soryz bacterial spots, were used as test-cultures: 211141a, 211141, 210341, 21034, and 210521, along\\nwith the collection strains of phytopathogens: Pseudomonas syringae 8299, Pseudomonas syringae subsp. syringae\\nUKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. The antagonistic activity of\\nthe extracted isolates of spore-forming and lactic acid bacteria was studied using the method of radial strokes (joint\\ncultivation of the antagonist and the strains under investigation). The bacterial isolates were deemed inactive if the\\ngrowth delay zone was 0–5 mm (–), from 5 to 10 mm (+) – low activity, 11–20 mm (++) – moderate activity, over 20\\nmm (+++) – high activity regarding the test-cultures. To check the effect of the isolate-antagonist of phytopathogenic\\nbacteria, artificial infecting was conducted in the field conditions. For this purpose, a diurnal culture of the antagonist\\nwas introduced into the stalk of plants in the concentration of 1×108 colony-forming units, and 24 h later, a culture of\\ntest-strain of the phytopathogen was administered above the previous puncture. The results were evaluated 7–14 days\\nafter the artificial infection. The experiment had three repeats. The isolates of bacteria which demonstrated their an-\\ntagonistic properties regarding the phytopathogenic bacteria were identified by their morphological properties, Gram\\nstaining, catalase test, profile of carbohydrate fermentation and mass-spectrometry (MALDI-TOF – Matrix Assisted\\nLaser Desorption/Ionization) using VITEK MS mass-spectrometer. Results. Thirty-eight spore-forming bacterial iso-\\nlates were extracted from soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 demonstrated their antagonistic\\nactivity towards the investigated phytopathogenic bacteria. Isolate 21040 showed high antagonistic activity to most\\ntest-strains of P. syringae from soryz (the zone of negative culture – 23–30 mm) and lower activity regarding the\\ncollection cultures. Isolates B4 and AVS3 demonstrated their selective activity regarding the investigated phytopatho-\\ngens. Twenty isolates of lactic acid bacteria were extracted. Higher antagonistic activity was noted for the isolates of\\nlactic acid bacteria 8/1 and F1 to the strains of P. syringae, isolated from soryz and collection cultures. The highest\\nantagonistic activity of isolate 8/1 was noted regarding test-strains of P. syringae 210521 and X. oryzae 8375 (the\\nzone of negative culture – 40–35 mm). In the field conditions, the treatment of sorghum plants with F1 affected the\\npathological process that developed due to the impact of the phytopathogenic bacteria P. syringae, which led to the\\nreduction in disease symptoms. The taxonomic position of the isolates of bacteria, which seem to be promising for\\nthe control of disease agents, was determined. In terms of morphology of cells and colonies, the biochemical profile,\\nand mass-spectrometry MALDI-TOF, the spore-forming isolates 21040 and B4 were identified as Bacillus subtilis,\\nand ASV3 – as Bacillus vallismortis. The identified isolates of lactic acid bacteria were Lactobacillus pentosus F1\\nand Lactobacillus sakei 8/1. Conclusions. In addition to phytopathogenic bacteria, from soryz plants we isolated the\\nstrains of spore-forming bacteria Bacillus subtilis 21040, B4, Bacillus vallismortis AVS3 and such lactic acid bacteria\\nas Lactiplantibacillus pentosus and Lactobacillus sakei 8/1 (Latilactobacillus sakei 8/1), promising for the elaboration\\nof methods for the biocontrol of the agents of bacterial diseases.\",\"PeriodicalId\":55933,\"journal\":{\"name\":\"Agricultural Science and Practice\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2024-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Science and Practice\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15407/agrisp10.03.046\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Science and Practice","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15407/agrisp10.03.046","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

目的分离和鉴定具有拮抗特性的细菌,用于生物防治高粱和高粱作物的细菌性病害。研究方法研究于 2021-2023 年进行。从乌曼国立园艺大学实验农场(乌曼切尔卡瑟州)田间采集的高粱样品中分离出孢子形成细菌。乳酸菌是从索里兹植物中分离出来的。索里兹植物采集于私人地块,该地块位于乌曼区莫纳斯特里什切社区的特奥林村、弗拉基斯拉夫奇克村和克尼亚日基村之间,波斯霍特拉夫内夫村曾位于该地块上。共分析了 1250 份样本。在分离植物病原菌的同时,还从夏季成熟期的茄子表面分离了孢子形成菌和乳酸菌。乳酸菌拮抗剂的分离物也是从翻耕过的田地里采集的桑树冬茬茎秆内部分离出来的。体外测定了从不同生态环境中分离到的乳酸菌和孢子形成菌株对油菜和高粱作物的植物病原菌的拮抗活性。试验培养物使用的假单胞菌是油菜菌斑病的病原菌:211141a、211141、210341、21034 和 210521,以及收集的植物病原体菌株:syringaeUKMB-1021、X. oryzae 8375、Dickeya chrysanthemi 8683、Diskeya chryzanthemi 8683。使用径向描边法(拮抗剂和被研究菌株的联合培养)研究了提取的孢子形成菌和乳酸菌分离物的拮抗活性。如果细菌分离物的生长延迟区为 0-5 毫米(-)、5-10 毫米(+)--低活性、11-20 毫米(++)--中等活性、20 毫米以上(+++)--试验培养物的高活性,则被视为无活性。为了检验植物病原菌分离拮抗剂的效果,在田间条件下进行了人工感染。为此,以 1×108 个菌落形成单位的浓度将拮抗剂昼夜培养物引入植物茎秆中,24 小时后,在前一次穿刺的上方注入植物病原菌的试验菌株培养物。人工感染 7-14 天后对结果进行评估。实验重复了三次。通过形态学特性、革兰氏染色、过氧化氢酶测试、碳水化合物发酵曲线以及使用 VITEK MS 质谱仪进行的质谱分析(MALDI-TOF - 基质辅助激光解吸/电离),对证明具有植物病原菌拮抗特性的细菌分离物进行了鉴定。结果。从 soryz 中提取出了 38 种孢子形成细菌等位基因,其中 21030、21095、21040、ASV1、ASV3 和 B4 对所研究的植物病原菌具有拮抗活性。21040 号菌株对来自 soryz 的 P. syringae 大多数菌株表现出较高的拮抗活性(阴性培养区 - 23-30 mm),而对采集培养物的活性较低。分离物 B4 和 AVS3 对所研究的植物病原菌具有选择性活性。提取了 20 个乳酸菌分离物。分离出的乳酸菌 8/1 和 F1 对从 soryz 和收集培养物中分离出的 P. syringae 菌株具有较高的拮抗活性。分离菌株 8/1 对试验菌株 P. syringae 210521 和 X. oryzae 8375 的拮抗活性最高(阴性培养区 - 40-35 mm)。在田间条件下,用 F1 处理高粱植株会影响由于植物病原菌 P. syringae 的影响而产生的病理过程,从而导致病害症状减轻。确定了细菌分离物的分类位置,这些分离物似乎有望用于控制病原菌。根据细胞和菌落的形态、生化特征和质谱 MALDI-TOF,确定孢子形成分离物 21040 和 B4 为枯草芽孢杆菌,ASV3 为瓦利斯菌。经鉴定,分离出的乳酸菌为戊糖乳杆菌 F1 和清酒乳杆菌 8/1。结论除了植物病原菌外,我们还从 soryz 植物中分离出了芽孢形成菌 Bacillus subtilis 21040、B4、Bacillus vallismortis AVS3 以及五联乳杆菌 Lactiplantibacillus pentosus 和清酒乳杆菌 Lactobacillus sakei 8/1 (清酒乳杆菌 Latilactobacillus sakei 8/1)等乳酸菌菌株,这些菌株有望用于制定细菌病原菌生物控制方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bacteria-antagonists of the agents of soryz bacterial diseases
Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents of bacterial diseases of soryz (Sorghum oryzoidum) and sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming bacteria were isolated from the soryz samples, collected in the fields of the experimental farm of the Uman National Horticulture University (Cherkasy region, Uman). Lactic acid bacteria were isolated from soryz plants, collected in the private land plot, located between the villages of Teolyn, Vladyslavchyk, Kniazhyky in Monastyryshche com- munity, Uman district, where Pershotravneve hamlet used to be situated. A total of 1,250 samples were analyzed. The experiment had three repeats. Spore-forming and lactic acid bacteria were isolated from the surface of soryz plants on the firm ripe stage in summer while isolating phytopathogenic bacteria. The isolates of lactic acid bacteria- antagonists were also isolated from the inner part of winter stubble stalk of soryz, collected from the tilled field. The antagonistic activity of the strains of lactic acid bacteria and spore-forming bacteria, isolated from different ecological niches, to phytopathogens of soryz and sorghum crops was determined in vitro. The strains of Pseudomonas syringae, the agents of soryz bacterial spots, were used as test-cultures: 211141a, 211141, 210341, 21034, and 210521, along with the collection strains of phytopathogens: Pseudomonas syringae 8299, Pseudomonas syringae subsp. syringae UKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. The antagonistic activity of the extracted isolates of spore-forming and lactic acid bacteria was studied using the method of radial strokes (joint cultivation of the antagonist and the strains under investigation). The bacterial isolates were deemed inactive if the growth delay zone was 0–5 mm (–), from 5 to 10 mm (+) – low activity, 11–20 mm (++) – moderate activity, over 20 mm (+++) – high activity regarding the test-cultures. To check the effect of the isolate-antagonist of phytopathogenic bacteria, artificial infecting was conducted in the field conditions. For this purpose, a diurnal culture of the antagonist was introduced into the stalk of plants in the concentration of 1×108 colony-forming units, and 24 h later, a culture of test-strain of the phytopathogen was administered above the previous puncture. The results were evaluated 7–14 days after the artificial infection. The experiment had three repeats. The isolates of bacteria which demonstrated their an- tagonistic properties regarding the phytopathogenic bacteria were identified by their morphological properties, Gram staining, catalase test, profile of carbohydrate fermentation and mass-spectrometry (MALDI-TOF – Matrix Assisted Laser Desorption/Ionization) using VITEK MS mass-spectrometer. Results. Thirty-eight spore-forming bacterial iso- lates were extracted from soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 demonstrated their antagonistic activity towards the investigated phytopathogenic bacteria. Isolate 21040 showed high antagonistic activity to most test-strains of P. syringae from soryz (the zone of negative culture – 23–30 mm) and lower activity regarding the collection cultures. Isolates B4 and AVS3 demonstrated their selective activity regarding the investigated phytopatho- gens. Twenty isolates of lactic acid bacteria were extracted. Higher antagonistic activity was noted for the isolates of lactic acid bacteria 8/1 and F1 to the strains of P. syringae, isolated from soryz and collection cultures. The highest antagonistic activity of isolate 8/1 was noted regarding test-strains of P. syringae 210521 and X. oryzae 8375 (the zone of negative culture – 40–35 mm). In the field conditions, the treatment of sorghum plants with F1 affected the pathological process that developed due to the impact of the phytopathogenic bacteria P. syringae, which led to the reduction in disease symptoms. The taxonomic position of the isolates of bacteria, which seem to be promising for the control of disease agents, was determined. In terms of morphology of cells and colonies, the biochemical profile, and mass-spectrometry MALDI-TOF, the spore-forming isolates 21040 and B4 were identified as Bacillus subtilis, and ASV3 – as Bacillus vallismortis. The identified isolates of lactic acid bacteria were Lactobacillus pentosus F1 and Lactobacillus sakei 8/1. Conclusions. In addition to phytopathogenic bacteria, from soryz plants we isolated the strains of spore-forming bacteria Bacillus subtilis 21040, B4, Bacillus vallismortis AVS3 and such lactic acid bacteria as Lactiplantibacillus pentosus and Lactobacillus sakei 8/1 (Latilactobacillus sakei 8/1), promising for the elaboration of methods for the biocontrol of the agents of bacterial diseases.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Agricultural Science and Practice
Agricultural Science and Practice AGRICULTURE, MULTIDISCIPLINARY-
自引率
25.00%
发文量
6
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信