{"title":"用中国崖柏叶(Leucaena leucocephala (Lam.) De Wit.)乙醇提取物配制抗痤疮丙酸杆菌生长的去角质凝胶面膜","authors":"Arfiani Arifin, Natsir Djide, Nurhidayah Nurhidayah","doi":"10.25026/jsk.v6i1.2075","DOIUrl":null,"url":null,"abstract":"Propionibacterium acnes is one of the gram-positive bacteria that can lead to the development of acne. One of the plants with the potential to be an anti-acne agent is the Petai Cina leaf (Leucaena leucocephala (Lam.) De Wit.). This study aims to formulate a peel-off gel mask preparation of the ethanol extract of Petai Cina leaf (Leucaena leucocephala (Lam.) De Wit.) that meets the physical quality test requirements and to determine the antiacne activity of the formulation. The extraction method used was maceration with 96% ethanol solvent. From the obtained extract, peel-off gel mask preparations were made and tested for their minimum inhibitory concentration (MIC): FI (2.5%), FII (5%), and FIII (10%), Medi-klin® (positive control), and gel base (negative control). The antibacterial activity was tested using the well diffusion method. The results of this study showed that the peel-off gel mask preparations FI (2.5%), FII (5%), FIII (10%), and the gel base (negative control) met the physical quality test requirements. The results of the anti-acne activity testing against the growth of Propionibacterium acnes bacteria obtained concentrations of extract FI (2.5%) 18.53 mm, FII (5%) 18.85 mm, FIII (10%) 22.27 mm, Medi-klin® 26.43 mm, and the negative control of Petai Cina leaf 6 mm. This indicates that the ethanol extract of Petai Cinaleaf has inhibitory effects on the growth of Propionibacterium acnes bacteria as an anti-acne agent.\nKeywords: Chinese petai leaves (Leucaena leucocephala (Lam.) De Wit.), Peel-off gel mask, Propionibacterium acnes, Anti-acne\n \nAbstrak\nPropionibacterium acnes merupakan salah satu bakteri gram positif yang dapat mengakibatkan timbulnya jerawat. Salah satu tanaman yang berpotensi sebagai antijerawat adalah daun petai cina (Leucaena leucocephala (Lam.) De Wit.). Penelitian ini bertujuan untuk memformulasi sediaan masker gel peel-off ekstrak etanol daun petai cina (Leucaena leucocephala (Lam.) De Wit.) yang memenuhi syarat uji mutu fisik dan untuk mengetahui aktivitas antijerawat formulasi sediaan masker gel peel-off ekstrak etanol daun petai cina (Leucaena leucocephala (Lam.) De Wit.). Metode ekstraksi digunakan yaitu metode maserasi dengan pelarut etanol 96%. Ekstrak yang diperoleh dibuat sediaan masker gel peel-off yang telah di uji konsentrasi hambat minimumnya (KHM) dengan konsentrasi ekstrak FI (2,5%), FII (5%) dan FIII (10%), Medi-klin® (kontrol positif) dan basis gel (kontrol negatif). Pengujian aktivitas antibakteri menggunakan metode sumuran. Hasil penelitian sediaan masker gel peel-off FI (2,5%), FII (5%), FIII (10%) dan basis gel (kontrol negatif) memenuhi syarat uji mutu fisik. Hasil pengujian aktivitas antijerawat terhadap pertumbuhan bakteri Propionibacterium acnes diperoleh konsentrasi ekstrak FI (2,5%) 18,53 mm, FII (5%) 18,85 mm, FIII (10%) 22,27 mm, Medi-klin® 26,43 mm dan kontrol negatif daun petai cina 6 mm. Hal ini menunjukkan bahwa ekstrak etanol daun petai cina memiliki daya hambat terhadap pertumbuhan bakteri Propionibacterium acnes sebagai antijerawat.\nKata Kunci: Daun petai cina (Leucaena leucocephala (Lam.) De Wit.), Masker gel peel-off, Propionibacterium acnes, Antijerawat","PeriodicalId":473028,"journal":{"name":"Jurnal Sains dan Kesehatan","volume":"6 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Formulasi Masker Gel Peel-Off Ekstrak Etanol Daun Petai Cina (Leucaena leucocephala (Lam.) De Wit.) sebagai Antijerawat terhadap Pertumbuhan Bakteri Propionibacterium acnes\",\"authors\":\"Arfiani Arifin, Natsir Djide, Nurhidayah Nurhidayah\",\"doi\":\"10.25026/jsk.v6i1.2075\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Propionibacterium acnes is one of the gram-positive bacteria that can lead to the development of acne. One of the plants with the potential to be an anti-acne agent is the Petai Cina leaf (Leucaena leucocephala (Lam.) De Wit.). This study aims to formulate a peel-off gel mask preparation of the ethanol extract of Petai Cina leaf (Leucaena leucocephala (Lam.) De Wit.) that meets the physical quality test requirements and to determine the antiacne activity of the formulation. The extraction method used was maceration with 96% ethanol solvent. From the obtained extract, peel-off gel mask preparations were made and tested for their minimum inhibitory concentration (MIC): FI (2.5%), FII (5%), and FIII (10%), Medi-klin® (positive control), and gel base (negative control). The antibacterial activity was tested using the well diffusion method. The results of this study showed that the peel-off gel mask preparations FI (2.5%), FII (5%), FIII (10%), and the gel base (negative control) met the physical quality test requirements. The results of the anti-acne activity testing against the growth of Propionibacterium acnes bacteria obtained concentrations of extract FI (2.5%) 18.53 mm, FII (5%) 18.85 mm, FIII (10%) 22.27 mm, Medi-klin® 26.43 mm, and the negative control of Petai Cina leaf 6 mm. This indicates that the ethanol extract of Petai Cinaleaf has inhibitory effects on the growth of Propionibacterium acnes bacteria as an anti-acne agent.\\nKeywords: Chinese petai leaves (Leucaena leucocephala (Lam.) De Wit.), Peel-off gel mask, Propionibacterium acnes, Anti-acne\\n \\nAbstrak\\nPropionibacterium acnes merupakan salah satu bakteri gram positif yang dapat mengakibatkan timbulnya jerawat. Salah satu tanaman yang berpotensi sebagai antijerawat adalah daun petai cina (Leucaena leucocephala (Lam.) De Wit.). Penelitian ini bertujuan untuk memformulasi sediaan masker gel peel-off ekstrak etanol daun petai cina (Leucaena leucocephala (Lam.) De Wit.) yang memenuhi syarat uji mutu fisik dan untuk mengetahui aktivitas antijerawat formulasi sediaan masker gel peel-off ekstrak etanol daun petai cina (Leucaena leucocephala (Lam.) De Wit.). Metode ekstraksi digunakan yaitu metode maserasi dengan pelarut etanol 96%. Ekstrak yang diperoleh dibuat sediaan masker gel peel-off yang telah di uji konsentrasi hambat minimumnya (KHM) dengan konsentrasi ekstrak FI (2,5%), FII (5%) dan FIII (10%), Medi-klin® (kontrol positif) dan basis gel (kontrol negatif). Pengujian aktivitas antibakteri menggunakan metode sumuran. Hasil penelitian sediaan masker gel peel-off FI (2,5%), FII (5%), FIII (10%) dan basis gel (kontrol negatif) memenuhi syarat uji mutu fisik. Hasil pengujian aktivitas antijerawat terhadap pertumbuhan bakteri Propionibacterium acnes diperoleh konsentrasi ekstrak FI (2,5%) 18,53 mm, FII (5%) 18,85 mm, FIII (10%) 22,27 mm, Medi-klin® 26,43 mm dan kontrol negatif daun petai cina 6 mm. Hal ini menunjukkan bahwa ekstrak etanol daun petai cina memiliki daya hambat terhadap pertumbuhan bakteri Propionibacterium acnes sebagai antijerawat.\\nKata Kunci: Daun petai cina (Leucaena leucocephala (Lam.) 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引用次数: 0
摘要
痤疮丙酸杆菌是可导致痤疮发生的革兰氏阳性细菌之一。Petai Cina 叶(Leucaena leucocephala (Lam.) De Wit.)是具有抗痤疮潜力的植物之一。本研究旨在将佩泰西那叶(Leucaena leucocephala (Lam.) De Wit.)的乙醇提取物配制成符合物理质量测试要求的剥离凝胶面膜制剂,并测定该制剂的抗痤疮活性。采用的提取方法是用 96% 的乙醇溶剂浸泡。从提取物中提取出剥离凝胶面膜制剂,并测试其最低抑菌浓度(MIC):FI(2.5%)、FII(5%)和 FIII(10%)、Medi-klin®(阳性对照)和凝胶基质(阴性对照)。抗菌活性采用井扩散法进行测试。研究结果表明,剥离型凝胶面膜制剂 FI(2.5%)、FII(5%)、FIII(10%)和凝胶基质(阴性对照)符合物理质量测试要求。针对痤疮丙酸杆菌生长的抗痤疮活性测试结果显示,提取物 FI(2.5%)的浓度为 18.53 mm,FII(5%)的浓度为 18.85 mm,FIII(10%)的浓度为 22.27 mm,Medi-klin® 的浓度为 26.43 mm,而阴性对照的佩泰希娜叶的浓度为 6 mm。这表明七叶树叶乙醇提取物具有抑制痤疮丙酸杆菌生长的作用,可作为抗痤疮剂: 痘痘丙酸杆菌 痘痘丙酸杆菌(Propionibacterium acnes)是一种革兰氏阳性菌。痤疮丙酸杆菌是一种典型的革兰氏阳性酵母菌,它可以抑制痤疮丙酸杆菌的生长。它的主要用途是用于制造去角质乙醇面膜(Leucaena leucocephala (Lam.) De Wit.)的配方中添加了抗菌活性成分。使用浓度为 96% 的乙醇进行混合。剥离型掩蔽剂凝胶(KHM)与 FI (2.5%)、FII (5%) 和 FIII (10%)、Medi-klin®(阳性对照)和基础凝胶(阴性对照)混合使用。抗结核活性测定仪采用的是总结方法。剥离型 FI(2.5%)、FII(5%)、FIII(10%)和基础凝胶(阴性对照组)掩蔽剂的使用可提高对鱼类疾病的认识。对痤疮丙酸杆菌的抗痤疮活性测试结果显示,FI(2.5%)为 18.53 毫米,FII(5%)为 18.85 毫米,FIII(10%)为 22.27 毫米,Medi-klin® 为 26.43 毫米,而阴性对照为 6 毫米。Kata Kunci: 白千层(Leucaena leucocephala (Lam.) De Wit.
Formulasi Masker Gel Peel-Off Ekstrak Etanol Daun Petai Cina (Leucaena leucocephala (Lam.) De Wit.) sebagai Antijerawat terhadap Pertumbuhan Bakteri Propionibacterium acnes
Propionibacterium acnes is one of the gram-positive bacteria that can lead to the development of acne. One of the plants with the potential to be an anti-acne agent is the Petai Cina leaf (Leucaena leucocephala (Lam.) De Wit.). This study aims to formulate a peel-off gel mask preparation of the ethanol extract of Petai Cina leaf (Leucaena leucocephala (Lam.) De Wit.) that meets the physical quality test requirements and to determine the antiacne activity of the formulation. The extraction method used was maceration with 96% ethanol solvent. From the obtained extract, peel-off gel mask preparations were made and tested for their minimum inhibitory concentration (MIC): FI (2.5%), FII (5%), and FIII (10%), Medi-klin® (positive control), and gel base (negative control). The antibacterial activity was tested using the well diffusion method. The results of this study showed that the peel-off gel mask preparations FI (2.5%), FII (5%), FIII (10%), and the gel base (negative control) met the physical quality test requirements. The results of the anti-acne activity testing against the growth of Propionibacterium acnes bacteria obtained concentrations of extract FI (2.5%) 18.53 mm, FII (5%) 18.85 mm, FIII (10%) 22.27 mm, Medi-klin® 26.43 mm, and the negative control of Petai Cina leaf 6 mm. This indicates that the ethanol extract of Petai Cinaleaf has inhibitory effects on the growth of Propionibacterium acnes bacteria as an anti-acne agent.
Keywords: Chinese petai leaves (Leucaena leucocephala (Lam.) De Wit.), Peel-off gel mask, Propionibacterium acnes, Anti-acne
Abstrak
Propionibacterium acnes merupakan salah satu bakteri gram positif yang dapat mengakibatkan timbulnya jerawat. Salah satu tanaman yang berpotensi sebagai antijerawat adalah daun petai cina (Leucaena leucocephala (Lam.) De Wit.). Penelitian ini bertujuan untuk memformulasi sediaan masker gel peel-off ekstrak etanol daun petai cina (Leucaena leucocephala (Lam.) De Wit.) yang memenuhi syarat uji mutu fisik dan untuk mengetahui aktivitas antijerawat formulasi sediaan masker gel peel-off ekstrak etanol daun petai cina (Leucaena leucocephala (Lam.) De Wit.). Metode ekstraksi digunakan yaitu metode maserasi dengan pelarut etanol 96%. Ekstrak yang diperoleh dibuat sediaan masker gel peel-off yang telah di uji konsentrasi hambat minimumnya (KHM) dengan konsentrasi ekstrak FI (2,5%), FII (5%) dan FIII (10%), Medi-klin® (kontrol positif) dan basis gel (kontrol negatif). Pengujian aktivitas antibakteri menggunakan metode sumuran. Hasil penelitian sediaan masker gel peel-off FI (2,5%), FII (5%), FIII (10%) dan basis gel (kontrol negatif) memenuhi syarat uji mutu fisik. Hasil pengujian aktivitas antijerawat terhadap pertumbuhan bakteri Propionibacterium acnes diperoleh konsentrasi ekstrak FI (2,5%) 18,53 mm, FII (5%) 18,85 mm, FIII (10%) 22,27 mm, Medi-klin® 26,43 mm dan kontrol negatif daun petai cina 6 mm. Hal ini menunjukkan bahwa ekstrak etanol daun petai cina memiliki daya hambat terhadap pertumbuhan bakteri Propionibacterium acnes sebagai antijerawat.
Kata Kunci: Daun petai cina (Leucaena leucocephala (Lam.) De Wit.), Masker gel peel-off, Propionibacterium acnes, Antijerawat