{"title":"评估从真皮劳桑中提取的劳桑酮及其有机金属复合物在大鼠身上的伤口愈合潜力。","authors":"Purnima V , Chhaya Harihar Gadgoli","doi":"10.1016/j.prenap.2024.100035","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim of the study</h3><p>To study the utility of Lawsone along with its zinc and copper complexes for wound healing activity using excision and incision wound models in rats.</p></div><div><h3>Methods</h3><p>The complex of plant nathoquinone from <em>Lawsonia inermis</em> called Lawsone viz Law and the metals viz. copper and zinc were synthesized. Molecular Docking (with GSK 3β protein) and Molecular Dynamic studies were carried out. The wound healing activity was evaluated using excision and incision wound models in Albino Wistar rats. The six groups comprised disease control, vehicle control, and standard, groups treated with Lawsone (Law) and its Zn Complex (ZnLaw) and copper complex (CuLaw). The progress of excision wound healing was monitored through wound contraction and biochemical and antioxidant parameters along with histopathology of granulation tissue, while for incision wounds, breaking and tensile strength were determined.</p></div><div><h3>Results</h3><p>The molecular docking studies revealed that ZnLaw has the highest affinity with GSK 3β protein. The Molecular Dynamic study determined the stability of Law and its metal complexes with GSK 3β protein and was found to be stable in the environment. The test compounds Law, ZnLaw, and CuLaw displayed significant (p<0.05) wound contraction. There was a significant (P< 0.01) decrease in the period of epithelisation as compared to vehicle control. ZnLaw showed maximum reduction in the period of epithelisation (16.03±0.942). There was a significant improvement in the antioxidant parameters from the granulation tissues of wounds treated with Law, ZnLaw, and CuLaw. The metal complexes of Law viz. ZnLaw and CuLaw showed a remarkable increase in hydroxyproline and hexosamine content as compared to the control. Histopathological findings indicated re-epithelisation, neovascularization, and increased collagen deposition as compared to the disease control. The incision model also revealed a significant (P< 0.001) increase in the tensile strength and breaking strength of the healed tissue as compared to the control.</p></div><div><h3>Conclusion</h3><p>Law and its Zinc and copper complexes exhibited good potential in healing of both incision and excision wounds.</p></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"3 ","pages":"Article 100035"},"PeriodicalIF":0.0000,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of wound healing potential of lawsone from Lawsonia innermis and its organometallic complexes in rats\",\"authors\":\"Purnima V , Chhaya Harihar Gadgoli\",\"doi\":\"10.1016/j.prenap.2024.100035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aim of the study</h3><p>To study the utility of Lawsone along with its zinc and copper complexes for wound healing activity using excision and incision wound models in rats.</p></div><div><h3>Methods</h3><p>The complex of plant nathoquinone from <em>Lawsonia inermis</em> called Lawsone viz Law and the metals viz. copper and zinc were synthesized. Molecular Docking (with GSK 3β protein) and Molecular Dynamic studies were carried out. The wound healing activity was evaluated using excision and incision wound models in Albino Wistar rats. The six groups comprised disease control, vehicle control, and standard, groups treated with Lawsone (Law) and its Zn Complex (ZnLaw) and copper complex (CuLaw). The progress of excision wound healing was monitored through wound contraction and biochemical and antioxidant parameters along with histopathology of granulation tissue, while for incision wounds, breaking and tensile strength were determined.</p></div><div><h3>Results</h3><p>The molecular docking studies revealed that ZnLaw has the highest affinity with GSK 3β protein. The Molecular Dynamic study determined the stability of Law and its metal complexes with GSK 3β protein and was found to be stable in the environment. The test compounds Law, ZnLaw, and CuLaw displayed significant (p<0.05) wound contraction. There was a significant (P< 0.01) decrease in the period of epithelisation as compared to vehicle control. ZnLaw showed maximum reduction in the period of epithelisation (16.03±0.942). There was a significant improvement in the antioxidant parameters from the granulation tissues of wounds treated with Law, ZnLaw, and CuLaw. The metal complexes of Law viz. ZnLaw and CuLaw showed a remarkable increase in hydroxyproline and hexosamine content as compared to the control. Histopathological findings indicated re-epithelisation, neovascularization, and increased collagen deposition as compared to the disease control. The incision model also revealed a significant (P< 0.001) increase in the tensile strength and breaking strength of the healed tissue as compared to the control.</p></div><div><h3>Conclusion</h3><p>Law and its Zinc and copper complexes exhibited good potential in healing of both incision and excision wounds.</p></div>\",\"PeriodicalId\":101014,\"journal\":{\"name\":\"Pharmacological Research - Natural Products\",\"volume\":\"3 \",\"pages\":\"Article 100035\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Natural Products\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950199724000235\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199724000235","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of wound healing potential of lawsone from Lawsonia innermis and its organometallic complexes in rats
Aim of the study
To study the utility of Lawsone along with its zinc and copper complexes for wound healing activity using excision and incision wound models in rats.
Methods
The complex of plant nathoquinone from Lawsonia inermis called Lawsone viz Law and the metals viz. copper and zinc were synthesized. Molecular Docking (with GSK 3β protein) and Molecular Dynamic studies were carried out. The wound healing activity was evaluated using excision and incision wound models in Albino Wistar rats. The six groups comprised disease control, vehicle control, and standard, groups treated with Lawsone (Law) and its Zn Complex (ZnLaw) and copper complex (CuLaw). The progress of excision wound healing was monitored through wound contraction and biochemical and antioxidant parameters along with histopathology of granulation tissue, while for incision wounds, breaking and tensile strength were determined.
Results
The molecular docking studies revealed that ZnLaw has the highest affinity with GSK 3β protein. The Molecular Dynamic study determined the stability of Law and its metal complexes with GSK 3β protein and was found to be stable in the environment. The test compounds Law, ZnLaw, and CuLaw displayed significant (p<0.05) wound contraction. There was a significant (P< 0.01) decrease in the period of epithelisation as compared to vehicle control. ZnLaw showed maximum reduction in the period of epithelisation (16.03±0.942). There was a significant improvement in the antioxidant parameters from the granulation tissues of wounds treated with Law, ZnLaw, and CuLaw. The metal complexes of Law viz. ZnLaw and CuLaw showed a remarkable increase in hydroxyproline and hexosamine content as compared to the control. Histopathological findings indicated re-epithelisation, neovascularization, and increased collagen deposition as compared to the disease control. The incision model also revealed a significant (P< 0.001) increase in the tensile strength and breaking strength of the healed tissue as compared to the control.
Conclusion
Law and its Zinc and copper complexes exhibited good potential in healing of both incision and excision wounds.
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