藻类与人脐静脉内皮细胞的光合共培养系统:缓解缺氧和缺氧/复氧损伤的效果

IF 1.6 Q4 ENGINEERING, BIOMEDICAL
Donghu Lin, Yuanyuan Chen, Xinyu Tao, Xin Che, Shiyu Li, Shiyu Cheng, Shuxin Qu
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引用次数: 0

摘要

这是一种新开发的光合共培养系统,用于缓解缺氧和缺氧/复氧(H/R)损伤的人脐静脉内皮细胞(HUVECs)。以明胶和海藻酸钠分别作为正电荷和负电荷材料,通过逐层包裹法(LbL)将藻类小球藻(Chlorella vulgaris)包裹起来,以减缓其生长速度,同时不影响其光合产氧能力。然后,构建了 HUVECs 与自氧海藻酸钠水凝胶(海藻凝胶)的光合共培养系统,在二维或三维共培养方式中,海藻与 HUVECs 的最佳比例分别为 5:1 和 20:1。结果表明,与二维共培养相比,三维共培养 HUVECs 需要更多的氧气来生产藻类。共培养的藻凝胶能减轻缺氧和缺氧/复氧(H/R)处理的HUVECs在增殖、细胞内ROS和细胞迁移能力方面的氧化应激损伤。此外,藻凝胶还能下调缺氧诱导因子 1α (HIF-1α)和血管内皮生长因子(VEGF)在缺氧和 H/R 损伤 HUVEC 中的表达,从而改善缺氧和 H/R 损伤。这种光合共培养系统通过提供安全稳定的氧气,为修复缺氧和 H/R 损伤的细胞或组织提供了一种可行的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Photosynthetic co-culture system of algae and human umbilical vein endothelial cells: The effect on alleviating hypoxia and hypoxia/reoxygenation injury

Photosynthetic co-culture system of algae and human umbilical vein endothelial cells: The effect on alleviating hypoxia and hypoxia/reoxygenation injury

It is a developed photosynthetic co-culture system to alleviate the hypoxia and hypoxia/reoxygenation (H/R)-injured human umbilical vein endothelial cells (HUVECs). The algae, Chlorella vulgaris, were encapsulated to slow their growth while not affecting the photosynthetic oxygen-producing capacity by Layer-by-layer (LbL) using gelatin and sodium alginate as the positive and negative charges materials, respectively. Then, the photosynthetic co-culture system of HUVECs and self-oxygenating alginate hydrogel (Algae-gel) was constructed in which the optimal ratios between algae and HUVECs were 5:1 and 20:1 for a 2D or 3D co-cultured manner, respectively. It indicated that the 3D co-cultured manner of HUVECs needed more O2 by the production of algae than it did in a 2D co-cultured manner. The co-cultured Algae-gel could alleviate hypoxia and the oxidative stress injury of hypoxia and hypoxia/reoxygenation (H/R)-treated HUVECs in the proliferation, intracellular ROS and cellular migratory ability. In addition, the Algae-gel could downregulate the expression of hypoxia-inducible factors 1α (HIF-1α) and vascular endothelial growth factor (VEGF) of hypoxia and H/R-injured HUVECs due to the improvement of hypoxia and H/R injury. This photosynthetic co-culture system could offer a promising approach for repairing hypoxia and H/R-injured cells or tissue by providing safe and stable O2.

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来源期刊
Biosurface and Biotribology
Biosurface and Biotribology Engineering-Mechanical Engineering
CiteScore
1.70
自引率
0.00%
发文量
27
审稿时长
11 weeks
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