最大限度地发挥硝化酶的潜力:揭示其多样性、催化能力和多功能应用

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Shi-Peng Zhou , Ya-Ping Xue , Yu-Guo Zheng
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引用次数: 0

摘要

腈酶是一类独特的酶,在催化腈类化合物水解形成相应的羧酸方面发挥着关键作用。这些酶实体已在制药、农用化学品和精细化工等多个行业引起了广泛关注。此外,日益增长的环境压力也凸显了它们的重要性,促使它们成为生物降解和生物修复工作的前沿。尽管如此,天然硝化酶还是表现出一些固有的局限性,如热稳定性低、底物选择性窄、不适应不同的环境条件等。在过去的十年中,人们在阐明硝化细菌酶的结构基础和催化机理方面做出了巨大努力,为硝化细菌酶的工程化提供了基础。在调控具有理想催化特性的亚硝酸酶和将酶应用于工业生产方面取得了重大突破。这篇综述力图全面论述和总结与硝化酶有关的最新研究进展,尤其侧重于阐明硝化酶的结构属性、催化机制、催化特性和提高催化性能的策略。此外,研究还扩展到了工艺工程领域,以及氮豆酶的多种应用。此外,该书还展望了氮酶的未来发展趋势,为相关领域的研究和应用提供了重要指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications

Nitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting environmental pressures, propelling them into the forefront of biodegradation and bioremediation endeavors. Nevertheless, the natural nitrilases exhibit intrinsic limitations such as low thermal stability, narrow substrate selectivity, and inadaptability to varying environmental conditions. In the past decade, substantial efforts have been made in elucidating the structural underpinnings and catalytic mechanisms of nitrilase, providing basis for engineering of nitrilases. Significant breakthroughs have been made in the regulation of nitrilases with ideal catalytic properties and application of the enzymes for industrial productions. This review endeavors to provide a comprehensive discourse and summary of recent research advancements related to nitrilases, with a particular emphasis on the elucidation of the structural attributes, catalytic mechanisms, catalytic characteristics, and strategies for improving catalytic performance of nitrilases. Moreover, the exploration extends to the domain of process engineering and the multifarious applications of nitrilases. Furthermore, the future development trend of nitrilases is prospected, providing important guidance for research and application in the related fields.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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