Rina Su, Ying Liang, Hu Chen, Neha Sheoran, Wencan Ke, Jie Bai, Mengya Jia, Jie Zhu, Qiang Li, Qi Liu, Xiaojun Chen, Xusheng Guo
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引用次数: 0
摘要
本研究探讨了单独使用或与酿酒酵母菌联合使用产生外多糖(EPS)的菌株(植物乳杆菌 L75)对不同温度下贮藏的燕麦青贮饲料的发酵特性、抗氧化能力和微生物群落序列的影响。使用植物酵母菌和酿酒酵母菌(LS)处理的青贮饲料在贮藏 3 天后 pH 值和乳酸积累迅速下降(p < 0.05)。在 7-60 天的贮藏期间,无论贮藏温度如何,接种植物乳杆菌(L)的组显示出最低的 pH 值、最低的氨氮和最高的乳酸量。当燕麦青贮饲料储存在 15°C 时,LS 接种组的超氧化物歧化酶(SOD)活性高于对照组和 L 接种组。此外,与 L 接种组(33.26%)相比,联合接种组中的 Lactiplantibacillus 比例增加了 65.42%。真菌群落数据显示,在 15°C 下储存的对照组和接种 L 组中都有大量的卡氏青霉。这些结果表明,联合接种 L. plantarum L75 和 S. cerevisiae 提高了燕麦青贮饲料在 15°C 下的发酵质量,从而提出了一种在收获季节低温地区提高青贮饲料发酵质量的技术。
Investigating the efficacy of an exopolysaccharide (EPS)-producing strain Lactiplantibacillus plantarum L75 on oat silage fermentation at different temperatures
This study investigates the effectiveness of an exopolysaccharide (EPS)-producing strain (Lactiplantibacillus plantarum L75) alone or in combination with Saccharomyces cerevisiae on the fermentation characteristics, antioxidant capacities and microbial community successions of oat silage stored at various temperatures. A rapid decrease in pH and lactic acid accumulation was observed in silages treated with L. plantarum and S. cerevisiae (LS) as early as 3 days of ensiling (p < 0.05). Over the ensiling period of 7–60 days, L. plantarum (L)-inoculated groups showed the lowest pH, lowest ammonia nitrogen and the highest amount of lactic acid regardless of the storage temperatures. When the oat silage was stored at 15°C, LS-inoculated group exhibited a higher superoxide dismutase (SOD) activity than control and L-inoculated group. Furthermore, the proportion of Lactiplantibacillus in the combined inoculation group increased by 65.42% compared to the L-inoculated group (33.26%). Fungal community data revealed abundant Penicillium carneum in the control and L-inoculated groups stored at 15°C. Conclusively, these results showed that combined inoculation of L. plantarum L75 and S. cerevisiae improved the fermentation quality of oat silage at 15°C, thus proposing a technique for enhancing the fermentation quality of silage in regions with low temperatures during harvest season.
期刊介绍:
Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes