蛆中的硅藻;诊断溺水的潜在工具--初步研究。

IF 1.5 4区 医学 Q1 LAW
I K Badu, D Asante, E D Agyemang, K Kwaku Duah, C K Adokoh, E Girela-Lopez
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引用次数: 0

摘要

高度腐烂会导致软组织大量脱落,因此很难使用硅藻检测作为溺水调查的可靠诊断工具。如果腐尸昆虫幼虫的硅藻检测呈阳性,则可为克服腐烂难题提供重要帮助。研究确定了在严重腐烂的尸体上对腐肉昆虫幼虫进行硅藻测试的实用性。使用硝酸、K2Cr2O7 和盐酸的改良酸消化法,分别消化了在淡水和海水中溺死的取食小猪腐肉的蝇类幼虫。使用光学显微镜和数码相机对提取的硅藻颗粒进行分析和鉴定。从海水淹死的仔猪蛆中提取的硅藻与从海水(淹死培养基)中提取的硅藻相似。蝇蛆中的中心硅藻(200 ± 60 个硅藻/毫升)高于羽状硅藻(80 ± 20 个硅藻/毫升)。蝇蛆和水样中常见的分离硅藻包括 Coscinodiscus sp.和 Navicular spp.,但淡水溺死仔猪的蝇蛆中没有硅藻。这项研究的结果表明,硅藻检测仍然是一种可靠的诊断工具,可用于确定完全腐烂的尸体是否与溺水有关。这是首次从取食溺水尸体的蛆虫中分离出硅藻的研究。它为进一步研究蛆在溺水调查中的作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diatoms in maggots; a potential tool for drowning diagnosis - A preliminary study.

Advanced putrefaction causes extensive loss of soft tissue, rendering it difficult to use the diatom test as a reliable diagnostic tool for drowning investigations. A positive diatom test in carrion insect larvae may provide significant assistance in overcoming the challenge of decomposition. The studies determined the utility of diatom test in carrion larvae on severely decomposed bodies. A modified acid digestion method involving nitric acid, K2Cr2O7 and HCl, was used to digest the blowfly larvae feeding on piglet carrion previously drowned in freshwater and sea water, respectively. Extracted diatom frustules were analysed and characterised using light microscopy coupled to a digital camera. Diatoms recovered from maggots on sea-drowned piglets were similar to diatoms from sea water (drowning medium). Centric diatoms recovered in maggots were higher (200 ± 60 diatoms/ mL) than pennate diatoms (80 ± 20 diatoms/mL). Isolated diatoms common to both maggots and water samples included Coscinodiscus sp. and Navicular spp. Albeit, there were no diatoms recovered from maggots on freshwater-drowned piglets. The findings of this study suggest that the diatom test is still a reliable diagnostic tool to determine if drowning was involved in the death of a fully decomposed body. This is the first study that isolated diatoms from maggots feeding on drowned bodies. It serves as the basis for further research into the utility of maggots for drowning investigations.

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来源期刊
Medicine, Science and the Law
Medicine, Science and the Law 医学-医学:法
CiteScore
2.90
自引率
6.70%
发文量
53
审稿时长
>12 weeks
期刊介绍: Medicine, Science and the Law is the official journal of the British Academy for Forensic Sciences (BAFS). It is a peer reviewed journal dedicated to advancing the knowledge of forensic science and medicine. The journal aims to inform its readers from a broad perspective and demonstrate the interrelated nature and scope of the forensic disciplines. Through a variety of authoritative research articles submitted from across the globe, it covers a range of topical medico-legal issues. The journal keeps its readers informed of developments and trends through reporting, discussing and debating current issues of importance in forensic practice.
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