Rosa María Márquez-González, Anilú Margarita Saucedo-Sariñana, César de Jesús Tovar-Jacome, Patricio Barros-Núñez, Martha Patricia Gallegos-Arreola, Mario Humberto Orozco-Gutiérrez, Ignacio Mariscal-Ramírez, Tomas Daniel Pineda-Razo, Aldo Antonio Alcaraz-Wong, María Eugenia Marín-Contreras, Mónica Alejandra Rosales-Reynoso
{"title":"NME1 和 DCC 变异与墨西哥结直肠癌患者的易感性和肿瘤特征有关。","authors":"Rosa María Márquez-González, Anilú Margarita Saucedo-Sariñana, César de Jesús Tovar-Jacome, Patricio Barros-Núñez, Martha Patricia Gallegos-Arreola, Mario Humberto Orozco-Gutiérrez, Ignacio Mariscal-Ramírez, Tomas Daniel Pineda-Razo, Aldo Antonio Alcaraz-Wong, María Eugenia Marín-Contreras, Mónica Alejandra Rosales-Reynoso","doi":"10.1186/s43046-024-00213-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) ranks third in cancer incidence globally and is the second leading cause of cancer-related mortality. The nucleoside diphosphate kinase 1 (NME1) and netrin 1 receptor (DCC) genes have been associated with resistance against tumorigenesis and tumor metastasis. This study investigates the potential association between NME1 (rs34214448 G > T and rs2302254 C > T) and DCC (rs2229080 G > C and rs714 A > G) variants and susceptibility to colorectal cancer development.</p><p><strong>Methods: </strong>Samples from 232 colorectal cancer patients and 232 healthy blood donors underwent analysis. Variants were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology. Associations were assessed using odds ratios (OR), and the p values were adjusted with Bonferroni test.</p><p><strong>Results: </strong>Individuals carrying the G/T and T/T genotypes for the NME1 rs34214448 variant exhibited a higher susceptibility for develop colorectal cancer (OR = 2.68, 95% CI: 1.76-4.09, P = 0.001 and OR = 2.47, 95% CI: 1.37-4.47, P = 0.001, respectively). These genotypes showed significant associations in patients over 50 years (OR = 2.87, 95% CI: 1.81-4.54, P = 0.001 and OR = 2.99, 95% CI: 1.54-5.79, P = 0.001 respectively) and with early Tumor-Nodule-Metastasis (TNM) stage (P = 0.001), and tumor location in the rectum (P = 0.001). Furthermore, the DCC rs2229080 variant revealed that carriers of the G/C genotype had an increased risk for develop colorectal cancer (OR = 2.00, 95% CI: 1.28-3.11, P = 0.002) and were associated with age over 50 years, sex, and advanced TNM stages (P = 0.001).</p><p><strong>Conclusions: </strong>These findings suggest that the NME1 rs34214448 and DCC rs2229080 variants play a significant role in colorectal cancer development.</p>","PeriodicalId":17301,"journal":{"name":"Journal of the Egyptian National Cancer Institute","volume":"36 1","pages":"10"},"PeriodicalIF":2.1000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NME1 and DCC variants are associated with susceptibility and tumor characteristics in Mexican patients with colorectal cancer.\",\"authors\":\"Rosa María Márquez-González, Anilú Margarita Saucedo-Sariñana, César de Jesús Tovar-Jacome, Patricio Barros-Núñez, Martha Patricia Gallegos-Arreola, Mario Humberto Orozco-Gutiérrez, Ignacio Mariscal-Ramírez, Tomas Daniel Pineda-Razo, Aldo Antonio Alcaraz-Wong, María Eugenia Marín-Contreras, Mónica Alejandra Rosales-Reynoso\",\"doi\":\"10.1186/s43046-024-00213-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Colorectal cancer (CRC) ranks third in cancer incidence globally and is the second leading cause of cancer-related mortality. The nucleoside diphosphate kinase 1 (NME1) and netrin 1 receptor (DCC) genes have been associated with resistance against tumorigenesis and tumor metastasis. This study investigates the potential association between NME1 (rs34214448 G > T and rs2302254 C > T) and DCC (rs2229080 G > C and rs714 A > G) variants and susceptibility to colorectal cancer development.</p><p><strong>Methods: </strong>Samples from 232 colorectal cancer patients and 232 healthy blood donors underwent analysis. Variants were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology. Associations were assessed using odds ratios (OR), and the p values were adjusted with Bonferroni test.</p><p><strong>Results: </strong>Individuals carrying the G/T and T/T genotypes for the NME1 rs34214448 variant exhibited a higher susceptibility for develop colorectal cancer (OR = 2.68, 95% CI: 1.76-4.09, P = 0.001 and OR = 2.47, 95% CI: 1.37-4.47, P = 0.001, respectively). These genotypes showed significant associations in patients over 50 years (OR = 2.87, 95% CI: 1.81-4.54, P = 0.001 and OR = 2.99, 95% CI: 1.54-5.79, P = 0.001 respectively) and with early Tumor-Nodule-Metastasis (TNM) stage (P = 0.001), and tumor location in the rectum (P = 0.001). Furthermore, the DCC rs2229080 variant revealed that carriers of the G/C genotype had an increased risk for develop colorectal cancer (OR = 2.00, 95% CI: 1.28-3.11, P = 0.002) and were associated with age over 50 years, sex, and advanced TNM stages (P = 0.001).</p><p><strong>Conclusions: </strong>These findings suggest that the NME1 rs34214448 and DCC rs2229080 variants play a significant role in colorectal cancer development.</p>\",\"PeriodicalId\":17301,\"journal\":{\"name\":\"Journal of the Egyptian National Cancer Institute\",\"volume\":\"36 1\",\"pages\":\"10\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Egyptian National Cancer Institute\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s43046-024-00213-7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Egyptian National Cancer Institute","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s43046-024-00213-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景：结直肠癌（CRC）在全球癌症发病率中排名第三，是癌症相关死亡的第二大原因。核苷二磷酸激酶 1（NME1）和净蛋白 1 受体（DCC）基因与肿瘤发生和肿瘤转移的抵抗力有关。本研究调查了 NME1（rs34214448 G > T 和 rs2302254 C > T）和 DCC（rs2229080 G > C 和 rs714 A > G）变异与结直肠癌发病易感性之间的潜在关联：对 232 名结直肠癌患者和 232 名健康献血者的样本进行了分析。采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）方法鉴定变异。相关性用几率比（OR）进行评估，P值用Bonferroni检验进行调整：结果：NME1 rs34214448变异的G/T和T/T基因型携带者患结直肠癌的易感性较高（OR=2.68，95% CI：1.76-4.09，P=0.001；OR=2.47，95% CI：1.37-4.47，P=0.001）。这些基因型在 50 岁以上的患者中显示出明显的相关性（OR = 2.87，95% CI：1.81-4.54，P = 0.001 和 OR = 2.99，95% CI：1.54-5.79，P = 0.001），并与早期肿瘤-结节-转移（TNM）分期（P = 0.001）和肿瘤位置在直肠（P = 0.001）相关。此外，DCC rs2229080变异显示，G/C基因型携带者罹患结直肠癌的风险增加（OR = 2.00，95% CI：1.28-3.11，P = 0.002），且与50岁以上年龄、性别和TNM分期晚期有关（P = 0.001）：这些研究结果表明，NME1 rs34214448和DCC rs2229080变异在结直肠癌的发展中起着重要作用。
NME1 and DCC variants are associated with susceptibility and tumor characteristics in Mexican patients with colorectal cancer.
Background: Colorectal cancer (CRC) ranks third in cancer incidence globally and is the second leading cause of cancer-related mortality. The nucleoside diphosphate kinase 1 (NME1) and netrin 1 receptor (DCC) genes have been associated with resistance against tumorigenesis and tumor metastasis. This study investigates the potential association between NME1 (rs34214448 G > T and rs2302254 C > T) and DCC (rs2229080 G > C and rs714 A > G) variants and susceptibility to colorectal cancer development.
Methods: Samples from 232 colorectal cancer patients and 232 healthy blood donors underwent analysis. Variants were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology. Associations were assessed using odds ratios (OR), and the p values were adjusted with Bonferroni test.
Results: Individuals carrying the G/T and T/T genotypes for the NME1 rs34214448 variant exhibited a higher susceptibility for develop colorectal cancer (OR = 2.68, 95% CI: 1.76-4.09, P = 0.001 and OR = 2.47, 95% CI: 1.37-4.47, P = 0.001, respectively). These genotypes showed significant associations in patients over 50 years (OR = 2.87, 95% CI: 1.81-4.54, P = 0.001 and OR = 2.99, 95% CI: 1.54-5.79, P = 0.001 respectively) and with early Tumor-Nodule-Metastasis (TNM) stage (P = 0.001), and tumor location in the rectum (P = 0.001). Furthermore, the DCC rs2229080 variant revealed that carriers of the G/C genotype had an increased risk for develop colorectal cancer (OR = 2.00, 95% CI: 1.28-3.11, P = 0.002) and were associated with age over 50 years, sex, and advanced TNM stages (P = 0.001).
Conclusions: These findings suggest that the NME1 rs34214448 and DCC rs2229080 variants play a significant role in colorectal cancer development.
期刊介绍：
As the official publication of the National Cancer Institute, Cairo University, the Journal of the Egyptian National Cancer Institute (JENCI) is an open access peer-reviewed journal that publishes on the latest innovations in oncology and thereby, providing academics and clinicians a leading research platform. JENCI welcomes submissions pertaining to all fields of basic, applied and clinical cancer research. Main topics of interest include: local and systemic anticancer therapy (with specific interest on applied cancer research from developing countries); experimental oncology; early cancer detection; randomized trials (including negatives ones); and key emerging fields of personalized medicine, such as molecular pathology, bioinformatics, and biotechnologies.
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