对CaMV35S启动子驱动的OsMYB48转录因子在模式植物--Nicotiana tabacum L.中赋予抗非生物性胁迫能力的内部分析和转化。

IF 4.5 2区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yumna Ahmad, Saqlain Haider, Javed Iqbal, Sana Naseer, Kotb A Attia, Arif Ahmed Mohammed, Sajid Fiaz, Tariq Mahmood
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引用次数: 0

摘要

全球作物产量受到多种非生物胁迫的影响。高温、盐度和干旱胁迫是最严重的环境生长限制因素。为了提高作物产量,人们采用分子方法来确定影响胁迫相关现象的关键调节因子。据报道,MYB 转录因子(TF)是一种很有前途的防御蛋白,能抵御植物必须面对的不利条件。人们认为 MYB 转录因子具有不同的作用,如调节细胞生长和分化、激素信号转导、介导非生物胁迫反应等。为了获得重要的洞察力,我们对 OsMYB TF 与 21 种双子叶植物 MYB TF 和 10 种单子叶植物 MYB TF 进行了全面的体内分析。分析了它们的染色体位置、基因结构、蛋白结构域和基序。此外,还研究了它们之间的系统发育关系,并将蛋白质分为 A、B、C 和 D 四个基本组。基因结构分析表明,同一类蛋白质具有相似的内含子-外显子结构。启动子分析发现了主要的顺式作用元件,这些元件负责激素信号转导,并启动对非生物胁迫和光诱导机制的响应。利用农杆菌介导的转化方法将 OsMYB TF 转化到烟草中,以进一步分析在胁迫条件下该基因在植物不同部位的表达水平。总之,目前的研究揭示了 OsMYB TF 在植物中的进化和作用。未来的研究应侧重于通过靶向遗传修饰和 CRISPR/Cas9 介导的基因组编辑,阐明 MYB 转录因子在非生物胁迫耐受性中的功能作用。在阐明 MYB 转录因子所协调的调控网络方面,应用全局学方法和系统生物学将是不可或缺的,这将有助于开发对环境胁迫具有更强抗逆性的作物基因型。必须对这些基因工程或编辑过的作物进行严格的实地验证,以确定它们在促进可持续农业实践中的效用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-silico analysis and transformation of OsMYB48 transcription factor driven by CaMV35S promoter in model plant - Nicotiana tabacum L. conferring abiotic stress tolerance.

Global crop yield has been affected by a number of abiotic stresses. Heat, salinity, and drought stress are at the top of the list as serious environmental growth-limiting factors. To enhance crop productivity, molecular approaches have been used to determine the key regulators affecting stress-related phenomena. MYB transcription factors (TF) have been reported as one of the promising defensive proteins against the unfavorable conditions that plants must face. Different roles of MYB TFs have been suggested such as regulation of cellular growth and differentiation, hormonal signaling, mediating abiotic stress responses, etc. To gain significant insights, a comprehensive in-silico analysis of OsMYB TF was carried out in comparison with 21 dicot MYB TFs and 10 monocot MYB TFs. Their chromosomal location, gene structure, protein domain, and motifs were analyzed. The phylogenetic relationship was also studied, which resulted in the classification of proteins into four basic groups: groups A, B, C, and D. The protein motif analysis identified several conserved sequences responsible for cellular activities. The gene structure analysis suggested that proteins that were present in the same class, showed similar intron-exon structures. Promoter analysis revealed major cis-acting elements that were found to be responsible for hormonal signaling and initiating a response to abiotic stress and light-induced mechanisms. The transformation of OsMYB TF into tobacco was carried out using the Agrobacterium-mediated transformation method, to further analyze the expression level of a gene in different plant parts, under stress conditions. To summarize, the current studies shed light on the evolution and role of OsMYB TF in plants. Future investigations should focus on elucidating the functional roles of MYB transcription factors in abiotic stress tolerance through targeted genetic modification and CRISPR/Cas9-mediated genome editing. The application of omics approaches and systems biology will be indispensable in delineating the regulatory networks orchestrated by MYB TFs, facilitating the development of crop genotypes with enhanced resilience to environmental stressors. Rigorous field validation of these genetically engineered or edited crops is imperative to ascertain their utility in promoting sustainable agricultural practices.

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来源期刊
Gm Crops & Food-Biotechnology in Agriculture and the Food Chain
Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
8.10
自引率
10.30%
发文量
22
期刊介绍: GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments
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